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Method for improving resveratrol content in giant knotweed by two-step microbial conversion

A technology for microbial transformation and resveratrol, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problem of high cost, complex resveratrol process route, limited increase in resveratrol content, etc. problems, to achieve the effect of increasing the content, improving the utilization of resources, and high conversion rate

Inactive Publication Date: 2011-11-16
ZHANGJIAJIE XIANGHUI BIOLOGICAL
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Problems solved by technology

However, the process route of extracting resveratrol by enzymatic hydrolysis is relatively complicated, and the content of resveratrol in raw materials can be limited and the cost is high

Method used

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  • Method for improving resveratrol content in giant knotweed by two-step microbial conversion

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Experimental program
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Effect test

Embodiment 1

[0012] Embodiment 1: the method for improving resveratrol content in Polygonum cuspidatum by two-step microbial transformation method, the steps are as follows

[0013] (1) Pulverization of raw material of knotweed: firstly, the dried knotweed is pulverized to 80 mesh with a pulverizer;

[0014] (2) The first step of transformation: take 5kg of knotweed, add 4kg of water, which is 70% of the weight of the raw material of knotweed, to infiltrate the knotweed powder, and stir evenly; add 50g of high-temperature yeast, and place the soaked knotweed powder in a container whose temperature is controlled at 55°C Ferment at constant temperature for 25 hours to obtain Polygonum cuspidatum powder after the first step of fermentation; convert resveratrol analogs into polydatin or resveratrol, and the special microorganisms are high-temperature yeast or yeast + glucoamylase or high-temperature yeast + saccharification enzyme.

[0015] (3) The second step of transformation: o...

Embodiment 2

[0016] Embodiment 2: The special microorganism added is yeast 50g+glucoamylase 100g, other is the same as embodiment 1.

Embodiment 3

[0017] Embodiment 3: The special microorganism added is 50 g of high-temperature yeast + 100 g of glucoamylase, and the others are the same as in Embodiment 1.

[0018] The content changes of polydatin and resveratrol in Polygonum cuspidatum raw materials before and after fermentation are shown in the following table:

[0019]

[0020] It can be seen from the comparison in the above table that the content of resveratrol in Polygonum cuspidatum can be greatly increased by using the two-step conversion method.

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Abstract

The invention provides a method for improving the resveratrol content in giant knotweed by two-step microbial conversion, comprising the following steps of: firstly, pulverizing dried giant knotweed into powder of 80 meshes, adding water for soaking, putting a special microbe and the soaked giant knotweed powder into a container with the temperature controlled at 35-60 DEG C, fermenting at constant temperature for 12-30 hours to convert the resveratrol analogue into polydatin or resveratrol; and then, adding low-temperature yeast, reducing the temperature to 20-30 DEG C, and fermenting at constant temperature for 30-48 hours to convert the polydatin into resveratrol. The method provided by the invention can greatly improve the resveratrol content in a giant knotweed material, is simple and easy to operate, reduces the production cost, and has a stable conversion process, high resveratrol conversion rate, simple equipment and small investment.

Description

technical field [0001] The invention relates to the technical field of microbial transformation and extraction of plant active ingredients, in particular, it provides a method for increasing the content of resveratrol in raw materials of traditional Chinese medicine Polygonum cuspidatum. technical background [0002] Resveratrol (Resveratrol), the chemical name is 3,4 ， , 5-trihydroxystilbene, has obvious antibacterial, anti-inflammatory, anti-cancer, anti-thrombotic, anti-hyperlipidemia, anti-lipid side oxidation and other pharmacological activities, widely used in health care products, food, cosmetics, etc. field. It widely exists in plants such as grape family, encyclopedia, leguminous family, umbelliferous family and sedge family. Studies have found that resveratrol has the highest content in Polygonum cuspidatum, and Polygonum cuspidatum also contains polydatin, the glucoside of resveratrol. Chinese invention patent application 99115156.9 discloses "A Process for Ex...

Claims

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Application Information

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IPC IPC(8): C12P7/22C07C39/21C07C37/68
Inventor 于华忠张世平张富谷雷献阳黄琳琳白冰
Owner ZHANGJIAJIE XIANGHUI BIOLOGICAL
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