Efficient bioconversion fermentation process for producing corydalmine

A technology of Viola damamin and biotransformation, applied in the biological field, can solve problems such as limited application and limited natural sources, and achieve the effects of simple operation, convenient large-scale production and high yield

Active Publication Date: 2011-11-16
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Violet Damamine is a natural trace active ingredient with good analgesic activity, which has the characteristics of high-efficiency and rapid analgesia, but its natural sources are very limited, which limits its application in the pharmaceutical field

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Inoculate the slant (i.e. strain) in the seed medium of the shaker flask, cultivate it at 28°C for 48 hours, the shaker speed is 180r / min; put it into the fermenter according to 6% inoculation amount, at 28°C, pH 6.0, ventilate After 24 hours of fermentation and cultivation under the condition of 1:1.2 and stirring speed of 180r / min, the conversion substrate tetrahydropalmatine was added, and the conversion and fermentation was continued for 24 hours before feed was fed, and then the conversion and fermentation was continued for 72 hours. HPLC detected that the yield of converting tetrahydropalmatine to produce Corydalis was 62%.

[0017] Transformation fermentation medium was prepared according to conventional methods (30 parts of corn flour, 7.6 parts of soybean meal, 5 parts of yeast powder, KH 2 PO 4 5 parts, NaCl 5 parts, FeSO 4 .7H 2 O 0.1 part, distilled water 1000ml, at pH6.0)

Embodiment 2

[0019] Inoculate the slant (i.e. strain) in the seed medium of the shaker flask, cultivate it at 28°C for 48 hours, the shaker speed is 180r / min; put it into the fermenter according to 6% inoculation amount, at 28°C, pH 6.0, ventilate Quantity 1:1.2, under the condition of stirring speed 180r / min, after 20 hours of fermentation and cultivation, the transformation substrate tetrahydropalmatine was added, the transformation and fermentation was continued for 20 hours, and then the feed was fed, and then the transformation and fermentation was continued for 72 hours. The yield of cordidamine produced from the conversion of tetrahydropalmatine by HPLC was 55%.

[0020] Transformation fermentation medium was prepared according to conventional methods (30 parts of starch, 7 parts of soybean powder, 4 parts of yeast powder, KH 2 PO 4 5 parts, NaCl5 parts, FeSO 4 .7H 2 O 0.1 part, distilled water 1000ml, at pH6.5)

Embodiment 3

[0022] Inoculate the slant (i.e. strain) in the seed medium of the shaker flask, cultivate it at 28°C for 48 hours, the shaker speed is 180r / min; put it into the fermenter according to 6% inoculation amount, at 28°C, pH 6.0, ventilate Quantity 1: 1.2, stirring speed 180r / min under the condition of fermentation and cultivation for 20 hours, add the conversion substrate Corydalis total alkaloids, continue the transformation and fermentation for 24 hours, then feed, and then continue the transformation and fermentation for 72 hours. The yield of cordidamine produced from the conversion of tetrahydropalmatine by HPLC was 50%.

[0023] Transformation fermentation medium was prepared by conventional method (28 parts of starch, 8 parts of soybean powder, 5 parts of yeast powder, KH 2 PO 4 5 parts, NaCl5 parts, FeSO 4 .7H 2 O 0.1 part, distilled water 1000ml, at pH6.5)

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Abstract

The invention relates to a bioconversion fermentation process for efficiently producing corydalmine. The process includes steps of: using a streptomyces griseus with a preservation number of ATCC 13273, converting L-tetrahydropalmatine in a culture medium comprising 30 parts of corn flour, 7.6 parts of bean pulp, 5 parts of yeast powder, 5 parts of KH2PO4, 5 parts of NaCl, 0.1 part of FeSO4.7H2O and 1000ml of distilled water. The conversion product is the corydalmine. The conversion and fermentation temperature is 28 DEG C. The pH is 6.0. The aerating capacity is 1:1.2. The inoculum concentration is 6% and the stirring rate is 180r/minute. The bioconversion fermentation is carried out for four days under above conditions.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a biotransformation and fermentation production process of Corydalis damming. Background technique [0002] Violet Damamine is a natural trace active ingredient with good analgesic activity, and has the characteristics of high-efficiency and rapid analgesia, but its natural sources are very limited, which limits its application in the pharmaceutical field. We have previously reported the method of biotransformation preparation of Levocordydamine (Pharmaceutical Biotechnology, 2003, 10 (3): 165-168), and applied for the biotransformation preparation method of Corydalamine and its application in the preparation of analgesics The invention patent (Chinese patent ZL 02138363.4) for the application in China discloses a method for preparing Corydalis damming by transformation and fermentation in shake flasks. In order to improve the biotransformation and production of natural ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/545
Inventor 余伯阳徐田刘吉华
Owner CHINA PHARM UNIV
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