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Stenotrophomonas maltophilia for degrading high-concentration methylbenzene and application of stenotrophomonas maltophilia

A technology of oligotrophomonas and maltophilia, which is applied in the field of microorganisms and can solve problems such as the inability to meet the needs of bioremediation

Active Publication Date: 2011-11-23
TSINGHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is far from meeting the needs of bioremediation under high-concentration toluene pollution conditions

Method used

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  • Stenotrophomonas maltophilia for degrading high-concentration methylbenzene and application of stenotrophomonas maltophilia
  • Stenotrophomonas maltophilia for degrading high-concentration methylbenzene and application of stenotrophomonas maltophilia
  • Stenotrophomonas maltophilia for degrading high-concentration methylbenzene and application of stenotrophomonas maltophilia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the isolation of Stenotrophomonas maltophilia

[0031] In a 250ml serum bottle, add 50ml of inorganic salt medium, the medium composition includes Na 2 HPO 4 5.65g / L, KH 2 PO 4 5.44g / L, (NH 4 ) 2 SO 4 1.00g / L, nitrilotriacetic acid 0.2g / L, MgSO 4 0.29g / L, CaCl 2 2H 2 O 0.07g / L, (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.19g / L, FeSO 4 ·7H 2O 8.3mg / L, disodium edetate 3.1mg / L, ZnSO 4 ·7H 2 O 13.7mg / L, MnSO 4 ·7H 2 O 1.9mg / L, CuSO 4 ·5H 2 O 0.5mg / L, Co(NO 3 ) 2 ·6H 2 O 0.3mg / L, Na 2 B 4 o 7 10H 2 O 0.2mg / L. The medium was sterilized at 121°C for 20 minutes. By aseptic technique, 0.1% (v / v) toluene was added to the serum bottle.

[0032] Take 2ml of aerobic activated sludge from Gaobeidian Sewage Treatment Plant, and aseptically add it to the above-mentioned 250ml serum bottle. The serum bottle was closed with a cap containing a gel pad, and the culture system was further sealed with a Parafilm film. The serum bottle was placed in an incubato...

Embodiment 2

[0039] Example 2, the growth of Stenotrophomonas maltophilia CGMCC No.4839 using toluene as the only carbon source and energy source (consistent with the name in the preservation certificate)

[0040] In a 250ml serum bottle, add 25ml of inorganic salt medium, the medium composition includes Na 2 HPO 4 5.65g / L, KH 2 PO 4 5.44g / L, (NH 4 ) 2 SO 4 1.00g / L, nitrilotriacetic acid 0.2g / L, MgSO 4 0.29g / L, CaCl 2 2H 2 O 0.07g / L, (NH 4 ) 6 Mo 7 o 24 .4H 2 O 0.19g / L, FeSO 4 ·7H 2 O 8.3mg / L, disodium edetate 3.1mg / L, ZnSO 4 ·7H 2 O 13.7mg / L, MnSO 4 ·7H 2 O 1.9mg / L, CuSO 4 ·5H 2 O 0.5mg / L, Co(NO 3 ) 2 ·6H 2 O 0.3mg / L, Na 2 B 4 o 7 10H 2 O 0.2mg / L. The medium was sterilized at 121°C for 20 minutes. By aseptic operation, add 15, 25, 35, 45 and 55 μl of toluene to the serum bottle, and insert 2 ml of 25 ml inorganic salt medium (250 ml serum bottle) containing 182.4 mg / L toluene to pre-culture for 2 days (30 ° C, Stenotrophomonas maltophilia CGMCC No.4839 cultur...

Embodiment 3

[0042] Embodiment 3, growth of Stenotrophomonas maltophilia CGMCC No.4839 at different temperatures

[0043] In a 250ml serum bottle, add 25ml of inorganic salt medium, the medium composition includes Na 2 HPO 4 5.65g / L, KH 2 PO 4 5.44g / L, (NH 4 ) 2 SO 4 1.00g / L, nitrilotriacetic acid 0.2g / L, MgSO 4 0.29g / L, CaCl 2 2H 2 O 0.07g / L, (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.19g / L, FeSO 4 ·7H 2 O 8.3mg / L, disodium edetate 3.1mg / L, ZnSO 4 ·7H 2 O 13.7mg / L, MnSO 4 ·7H 2 O 1.9mg / L, CuSO 4 ·5H 2 O 0.5mg / L, Co(NO 3 ) 2 ·6H 2 O 0.3mg / L, Na 2 B 4 o 7 10H 2 O 0.2mg / L. The medium was sterilized at 121°C for 20 minutes. By aseptic operation, add 25 μl of toluene to the serum bottle, and insert 2 ml of oligotrophic monotrophs of maltophilia pre-cultured for 2 days (30°C, 150 rpm) in 25 ml of inorganic salt medium (250 ml of serum bottle) containing 182.4 mg / L toluene Bacteria CGMCC No.4839 culture solution. OD after inoculation 600 is 0.20, and the toluene liquid phase co...

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Abstract

The invention discloses a Stenotrophomonas maltophilia for degrading high-concentration methylbenzene and application of the Stenotrophomonas maltophilia. The Stenotrophomonas maltophilia is named as the specifications and has the collection number of CGMCC (China General Microbiological Culture Collection Center) No.4839. The invention also discloses a method for degrading methylbenzene by using the Stenotrophomonas maltophilia with the collection number of CGMCC No.4839. The Stenotrophomonas maltophilia with the collection number of CGMCC No.4839 can degrade the methylbenzene at high concentration, can grow by taking the methylbenzene as the unique carbon source and energy, and has high capacity of degrading, utilizing and tolerating the methylbenzene.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Stenotrophomonas maltophilia which can degrade high-concentration toluene and use toluene as the only carbon source and energy source for growth and its application. Background technique [0002] Toluene is an important organic solvent and a widely used basic chemical raw material, which is produced and used in large quantities in industry. Due to leakage and improper disposal, toluene has become an important pollutant widely discharged from many industrial point sources, and has become one of the main pollutants existing in air, water and other environments. Toluene is volatile, but toluene is difficult to completely remove from the aqueous environment and may exist for a long time. Toluene is highly toxic. Exposure to a certain concentration of toluene will have a serious impact on human health. The main toxic effect of toluene is to cause damage to the cent...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/01A62D101/20
Inventor 喻国策王建龙
Owner TSINGHUA UNIV
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