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A kind of freeze-drying protective agent, preparation method and application thereof

A drying protective agent and drying technology, applied in the direction of biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve problems such as development lag, long-term storage of finished products, and transportation restrictions

Active Publication Date: 2011-11-30
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the development of traditional domestic biological product protective agents is relatively lagging behind, which limits the long-term storage and transportation of finished products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The components of the freeze-drying protective agent are as shown in formula 1 in table 4,

[0038] Preparation method following steps:

[0039] 1) Mix according to the above formula 1 to make a freeze-drying protective agent;

[0040] 2) Sterilize the above-mentioned freeze-drying protective agent at a temperature of 100° C. for 30 minutes.

[0041] Applying the above-prepared freeze-drying protective agent to Lactococcus has a better protective effect on Lactococcus during freeze-drying.

[0042] To prepare the lactic acid coccus starter with the above-mentioned freeze-drying protective agent, first, add the Streptococcus thermophilus enriched by centrifugation to the above-mentioned freeze-drying protective agent in a mass ratio of 1:1, and mix the two in a sterile state uniform. Then the prepared Streptococcus thermophilus proliferation medium was stored in an environment of 4° C. for 12 hours.

[0043] The Streptococcus thermophilus freeze-dried starter produce...

Embodiment 2

[0047] The composition of freeze-drying protective agent is as shown in formula 2 in table 4,

[0048] Preparation method is as embodiment 1:

[0049] Applying the above-prepared freeze-drying protective agent to Lactococcus has a better protective effect on Lactococcus during freeze-drying.

[0050]To prepare the lactic acid coccus starter with the above-mentioned freeze-drying protective agent, first, add the Streptococcus thermophilus enriched by centrifugation to the above-mentioned freeze-drying protective agent in a mass ratio of 1:1, and mix the two in a sterile state uniform. Then the prepared Streptococcus thermophilus proliferation medium was stored in an environment of 4° C. for 12 hours.

[0051] The Streptococcus thermophilus freeze-dried starter produced by the above-mentioned freeze-drying protective agent was inoculated in an appropriate amount in sterilized whole milk with a dry matter content of 11.5%, and the fermentation experiment was carried out at 43°C...

Embodiment 3

[0054] The composition of freeze-drying protective agent is as shown in formula 3 in table 4,

[0055] Preparation method is as embodiment 1:

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PUM

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Abstract

The invention relates to a freeze-drying protective agent which is prepared from the following components in parts by weight: 10-18 parts of skimmed milk powder, 0.5-2 parts of sodium glutamate, 0.3-1.5 parts of sodium ascorbate, 0.5-3 parts of glycerol, 2-10 parts of sucrose, 10-20 parts of trehalose and the balance of water. The invention also relates to a preparation method of the freeze-drying protective agent, which comprises the following steps: mixing the components, and sterilizing at 100 DEG C for 30 minutes to obtain the freeze-drying protective agent. The freeze-drying protective agent is used for freeze-drying microbes, especially lactic acid coccus. The invention maximally displays the protective function of the freeze-drying protective agent, reduces the death of coccus in the freeze-drying process, and greatly increases the survival rate of coccus.

Description

technical field [0001] The invention relates to a freeze-drying protective agent, in particular to a freeze-drying protective agent for lactococcus, a preparation method and application of the freeze-drying protective agent. Background technique [0002] Freeze-drying is a drying process in which wet materials are frozen into a solid state at a lower temperature, and then the solid moisture is directly sublimated into a gaseous state under high vacuum to remove it, also known as sublimation drying. In the process of freeze-drying, the two processes of freezing and drying will cause damage, death and passivation of certain enzyme protein molecules of some microbial cells. If the protective agent is not properly selected in the freeze-drying process, the fermentation activity will decrease. [0003] Lactococcus is one of the important strain resources in the dairy industry microorganisms. It has good fermentation characteristics and improves or regulates the balance of intest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 刘先龄南康小红生庆海刘卫星
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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