Detection method capable of reducing background influence based on waveguide coupling surface plasma resonance

Abstract

The invention discloses a detection method capable of reducing background influence based on waveguide coupling surface plasma resonance. A waveguide coupling surface plasma resonance peak and a neighboring waveguide coupling resonance peak thereof with higher sensitivity are selected according to the principle that the waveguide coupling surface plasma resonance peak and the waveguide coupling resonance peak have different sensitivities to the variation of external matters including a label layer and a background layer in an intensity curve of the reflected light responded by a waveguide coupling surface plasma resonance sensor, and two equations respectively corresponding to the variation of a sample to be detected and the position offset of the two peaks are established. The amount of variation of the label layer and the background layer can be respectively obtained by solving the system of linear equations with two unknowns, so that interference of the background layer is reduced.The detection method needs no additional reference channel, and can accurately detect the sample to be detected in simple structure, so that the interference on the background layer of the sample to be detected is reduced, accuracy and reliability of the detection system are increased, and implementation of high-throughput detection can be facilitated.

Description

technical field [0001] The invention relates to the field of sensors and sensing technologies. In particular, it relates to a detection method for reducing background influence based on waveguide coupling surface plasmon resonance. Background technique [0002] Surface electrons in metal or semiconductor materials behave like a gas of free electrons. Surface plasmon waves are vibrations that are formed by the collective oscillation of free charges on the metal surface and propagate along the interface between the metal and the dielectric. Surface plasmon waves exist at the interface of two media with opposite permittivity signs (generally metal and medium). Its field strength reaches its maximum at the interface, and decays exponentially along the direction perpendicular to the interface on both sides of the interface, so that the field is confined near the interface. Surface plasmon resonance (SPR) is a physical optical phenomenon. The wave vector component perpendicular...

AI Technical Summary

Problems solved by technology

Sometimes the non-specific response of the background layer is even greater than the specific response of the label layer, so that if there is no reference channel, it is impossible to distinguish what kind of response caused the change of the surface plasmon resonance condition, which has a great impact on the judgment and analysis of the detection results

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