Method for determining the value of cryiab protein standard substance

A standard material, protein technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve the effect of improving reliability, ensuring uniformity, and accurate quantification results

Active Publication Date: 2011-11-30
NAT INST OF METROLOGY CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the actual detection work, it is often necessary to perform qualitative and quantitative detection of CryIAb protein on the genetically modified crops to be detected, but there are still few studies on whether the value of its standard substance is accurate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) CryIAb standard substance preparation process:

[0025] (1) A 0.1% ammonia solution was prepared for dissolving the purified CryIAb.

[0026] (2) Prepare a CryIAb solution with a concentration of 50 μg / g.

[0027] (3) Divide the CryIAb solution into 500 μL cryopreservation tubes, 400 μL solution into each cryopreservation tube, and store the subpackaged standard substances in a -80°C refrigerator.

[0028] (2) Determination of standard substances:

[0029] (1) Three specific peptides of LIGNYTDHAVR, TLSSTLR, and IVAQLGQGVYR were selected for CryIAb protein quantification.

[0030] (2) The above three peptides were synthesized, and the three corresponding isotope-labeled peptides were synthesized using all deuterium-labeled leucamino groups at the same time.

[0031] (3) Use the national standard substances of leucine, isoleucine, valine, proline and / or phenylalanine as the standard, and label leucine, isoleucine, valine and proline with isotopes Acine and / or phe...

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Abstract

The invention provides a method for certifying a CryIAb protein standard substance. A Bacillus thuringiensis (BT) crystalline protein has high-specificity insecticidal activity and serves as biopesticide which is applied most widely in the recent years; and during actual detection work, genetically modified corps to be detected are often required to be subjected to CryIAb protein quantitative andquantificational detection, but the research on the accuracy of standard substance certification of the genetically modified corps is rare. The technical problem to be solved is to fill the technicalblank; and the invention provides the method for certifying the CryIAb protein standard substance, and the method is high in accuracy and has reliability and traceability.

Description

technical field [0001] The invention relates to a method for determining the value of a standard substance, in particular to a method for determining the value of an insecticidal crystal protein CryIAb standard substance. Background technique [0002] The measurement of the characteristic values ​​of samples that have passed the uniformity and stability tests is called characterization in the technical field of standard substances (the original text is characterize, that is, the measurement of material characteristics); The quantitative value of the characteristic is confirmed by metrology traceability (hereinafter referred to as traceability) and the rationality of the corresponding measurement uncertainty assessment. , generally referred to as authentication in other technical fields). Therefore, the determination of certified reference materials should actually be divided into two steps: first, measure the characteristic values ​​of the samples that have passed the unifo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 武利庆毕佳明宋德伟王晶杨彬
Owner NAT INST OF METROLOGY CHINA
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