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A method for extracting eucommia gum from eucommia leaves

A technology for eucommia gum and eucommia leaves, which is applied in the field of extracting eucommia gum from eucommia leaves by biological methods, can solve the problems of production efficiency, low production cost, low added output value, unutilized active ingredients, long production cycle, etc., and achieves reduction of infrastructure costs. , Reduce the amount of material handling, and promote the effect of rapid growth

Inactive Publication Date: 2011-12-14
武汉普赛特膜技术循环利用有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to propose a process scheme with high production efficiency, low production cost and high additional output value in view of the problems of long production cycle, low extraction efficiency and no utilization of other active ingredients in the existing Eucommia gum extraction process

Method used

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  • A method for extracting eucommia gum from eucommia leaves

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Embodiment 1

[0021] The first step: preprocessing. Take 20kg of dried leaves of Eucommia ulmoides, add 40kg of water, grind with a steel mill, then add 120kg of water and 0.1kg of protease, hydrolyze for 2 hours at a pH of 6 and a temperature of 55°C, and centrifuge.

[0022] The second step: nitrogen source preparation. The supernatant is filtered through a N100 microfiltration membrane to remove macromolecular proteins and suspended solids; the permeate is concentrated through a nanofiltration membrane, and the concentration ratio is 6 times; the concentrated solution is adsorbed by perforated adsorption resin for chlorogenic acid. Dried the under-column liquid and microfiltration concentrate to obtain protein and polypeptide and mixed them for use.

[0023] The third step: the extraction of Eucommia gum. Inoculate Eucommia ulmoides-16 in the slag obtained by centrifugation for solid-state fermentation, the inoculum size is 0.3kg, add the nitrogen source and glucose prepared in the pre...

Embodiment 2

[0025] The first step: preprocessing. Take 20kg of dried leaves of Eucommia ulmoides, add 40kg of water, grind with a steel mill, then add 120kg of water and 0.1kg of protease, hydrolyze for 2 hours at a pH of 6 and a temperature of 55°C, and centrifuge.

[0026] The second step: nitrogen source preparation. The supernatant is filtered through a N100 microfiltration membrane to remove macromolecular proteins and suspended solids; the permeate is concentrated through a nanofiltration membrane, and the concentration ratio is 6 times; the concentrated solution is adsorbed by perforated adsorption resin for chlorogenic acid. The under-column liquid and the microfiltration concentrate were dried to obtain a protein and polypeptide mixture for use.

[0027] The third step: the extraction of Eucommia gum. Inoculate Eucommia ulmoides-16 in the slag obtained by centrifugation for solid-state fermentation, the inoculum size is 0.3kg, add the prepared protein, polypeptide mixture and p...

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Abstract

The invention relates to a method for extracting natural products, in particular to a method for extracting eucommia gum from eucommia leaves by using a biological method. The following process steps are included: the first step is pretreatment of eucommia leaves, including pulping and enzymatic hydrolysis. The second step is the preparation of the nitrogen source, including membrane filtration, membrane concentration and resin adsorption, and drying the membrane filtration concentrate and the subcolumn liquid as the nitrogen source for later use. The third step: extraction of eucommia gum, including inoculation of Eucommia ulmoides-16 for solid-state fermentation, separation of solid and liquid from the fermentation product, drying of the liquid as a carbon source for future use, and light washing and refining of the crude gum to obtain fine gum. This method has high production efficiency, low production cost, and can co-produce chlorogenic acid to increase income.

Description

technical field [0001] The invention relates to a method for extracting natural products, in particular to a method for extracting eucommia gum from eucommia leaves by using a biological method. Background technique [0002] Eucommia gum is a white filamentous substance, its chemical composition is trans-1,4-polyisoprene, it has rubber-plastic duality, it is called the second natural rubber, and it has the widest adaptability and development potential in the world. The largest source of high-quality rubber is the only substitute for natural rubber, which can fundamentally solve the problem of shortage of natural rubber resources in my country. At present, the cultivation area of ​​eucommia ulmoides in my country is about 350,000 hectares, accounting for more than 99% of the world's eucommia ulmoides planting area. The development of the process of producing Eucommia gum with Eucommia leaves has become a hot spot. [0003] Prior to the present invention, there were some paten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/00C08L7/00C12S3/00
Inventor 万端极刘冬成徐国念吴正奇李丹刘芸芳魏旋杨青李猷
Owner 武汉普赛特膜技术循环利用有限公司
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