A method for rapid and non-destructive analysis of nutritional quality of whole cottonseed based on near-infrared spectroscopy

A near-infrared spectroscopy and non-destructive analysis technology, which is applied in the field of rapid and non-destructive analysis of the nutritional quality of whole cottonseeds based on near-infrared spectroscopy, can solve the problems of consuming chemical reagents, cumbersome sample preparation, and long analysis time, achieving high analysis accuracy and detection Fast speed and improved signal-to-noise ratio

Inactive Publication Date: 2011-12-14
ZHEJIANG UNIV
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Problems solved by technology

At present, the detection of these quality indicators is mainly based on conventional chemical methods, such as the Kjeldahl method for protein content detection, and the Soxhlet extraction method for oil content detection. These traditional analytical methods have high sensitivity and accuracy, but there are samples Problems such as cumbersome preparation, long analysis time, high detection cost, and large consumption of chemical reagents

Method used

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  • A method for rapid and non-destructive analysis of nutritional quality of whole cottonseed based on near-infrared spectroscopy
  • A method for rapid and non-destructive analysis of nutritional quality of whole cottonseed based on near-infrared spectroscopy
  • A method for rapid and non-destructive analysis of nutritional quality of whole cottonseed based on near-infrared spectroscopy

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Experimental program
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Embodiment

[0035] 1. Select 385 representative cottonseed materials as the experimental sample set, which were planted in Hainan Sanya, Zhejiang Hangzhou, Zhejiang Jinhua and other ecological planting areas in 2008 and 2009 respectively; 230 samples were selected as the calibration samples of the whole cottonseed The set is used to construct the correction model of the whole cottonseed, and the remaining 155 samples are used as the prediction sample set of the whole cottonseed to evaluate the performance of the correction model of the whole cottonseed;

[0036] 2. In the spectral range with a wavelength of 800-2500nm, use a near-infrared analyzer to scan the whole cottonseed sample, collect the near-infrared reflection spectrum intensity every 2nm, repeat the scan four times for each sample, and take the average spectrum as the whole cottonseed The near-infrared spectral data of the calibration sample set and the near-infrared spectral data of the predicted sample set of whole cottonseed,...

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Abstract

The invention provides a novel fast and non-destructive analysis method for the nutritional quality of cottonseed. The method comprises the following steps: 1) selecting whole cottonseeds of representative cotton varieties as experimental samples; 2) using a near-infrared spectrometer to collect near-infrared spectral data of whole cottonseeds, and the spectral scanning range is 800-2500nm; 3) using the national standard method Accurately analyze the nutritional quality of cottonseed; 4) Preprocess the near-infrared spectrum data; 5) Select spectral variables to eliminate non-informative variables; 6) Use the multivariate correction method to establish the near-infrared spectrum of whole cottonseed and the nutritional value of whole cottonseed 7) Performance evaluation of the calibration model; 8) Collect near-infrared spectral data of the whole cottonseed to be tested, and use the calibration model to predict its nutritional quality after preprocessing the spectral data. The invention has fast analysis speed, does not destroy samples, does not require sample pretreatment, has high detection accuracy, and can analyze various nutritional quality components in cotton seeds at the same time, and provides a convenient, fast and efficient analysis method for cotton breeding and cotton seed inspection.

Description

technical field [0001] The invention relates to a method for rapidly and nondestructively analyzing the nutritional quality of whole cottonseeds based on near-infrared spectroscopy. Specifically, it involves a method for rapid and non-destructive analysis of the nutritional quality protein and oil content of whole cottonseeds by using near-infrared spectroscopy and chemometric multivariate correction technology. Background technique [0002] Cottonseed is the most important by-product of cotton production, and its annual production is huge. According to statistics, for every 1kg of cotton fiber produced, 1.65kg of cottonseed will be produced. Cottonseed is rich in protein 27.83%-45.6% and oil 28.24%-44.05%, which is a huge edible oil and potential plant protein resource. The amino acid composition of cottonseed protein is reasonable. Except for the slightly lower content of methionine, other essential amino acids in cottonseed protein have reached the recommended standards...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N21/25
Inventor 祝水金黄庄荣陈进红何秋伶刘海英
Owner ZHEJIANG UNIV
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