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Application of Plant Poly-adp Ribose Polymerase Inhibitors in Improving Plant Traits

A technology of ribose polymerase and plant traits, applied in the field of plant biology and agriculture, can solve the problems of slow effect, time-consuming and labor-intensive

Inactive Publication Date: 2011-12-21
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, drought-resistant breeding is mainly achieved through cross-breeding or transgenic breeding to cultivate drought-resistant strains. At present, my country has invested a lot of money in this area, but the effect is slow, and for each species, due to differences in plant physiological forms and planting conditions, All need to invest corresponding research force to carry out research, which is time-consuming and labor-intensive, so it is very important to develop new ideas and methods in drought-resistant breeding (liu et al., 2006)

Method used

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  • Application of Plant Poly-adp Ribose Polymerase Inhibitors in Improving Plant Traits
  • Application of Plant Poly-adp Ribose Polymerase Inhibitors in Improving Plant Traits
  • Application of Plant Poly-adp Ribose Polymerase Inhibitors in Improving Plant Traits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Treatment method of Arabidopsis, tobacco, corn and other seeds

[0022] Arabidopsis thaliana and tobacco seeds: first sterilize with 70% ethanol for 10 minutes, then shake with 100% ethanol + 0.01% Triton for several times, pour it on sterilized clean filter paper, and plant it on 1 / 2MS medium with toothpicks. Put it in a light incubator for cultivation. Use the medium without 3-AB as a control to observe the growth of plants on the medium containing 1-2mM 3-AB or BA.

[0023] Corn seeds: Sterilize with 3% hydrogen peroxide for 30 minutes, rinse several times to wash hydrogen peroxide, soak seeds in clean pure water for 48h, 37°C for 24h, then arrange the yarn in a long test tube as a support, and place the germinated seedlings On gauze, there is 1 plant per tube, a set of ten tubes, the test tube does not contain or contains 3-AB or BA aqueous solution, and it is grown in a light incubator for one week and then photographed.

Embodiment 2

[0024] Example 2 Determination of the number of lateral roots and the fresh weight of plant parts

[0025] After 20 days of growth of Arabidopsis, take more than 15 seedlings, count the number of roots emitted from the base and the number of lateral roots of the entire root system; divide the seedlings into roots and parts above the roots, weigh the fresh weights with precision balances, compare the treatment and The number of lateral roots and the fresh weight of each part of the treated plants. The number of lateral roots of the entire root system of each plant was counted after six weeks of growth for tobacco, and the number of lateral roots that grew from the fibrous roots from the basal roots were counted for corn after one week of growth.

Embodiment 3

[0026] Example 3 Observation and Measurement of Plant Drought Resistance

[0027] When Arabidopsis germinates to the first true leaf, select robust and consistent seedlings and transplant them into pre-sterilized culture soil (the soil is first weighed to ensure the amount of soil in each pot during drought. The same, the water content is also the same). Five days after the Arabidopsis was transplanted, 3-AB was formulated into a 2mM aqueous solution, and the roots of the Arabidopsis were drip-irrigated, and each seedling should be treated with the same volume of solution as far as possible with the roots moist. Water drip irrigation was used as the control of 3-AB treatment. Repeat every 5 days for a total of 3 treatments. After the plants have grown for 30 days, let each small pot absorb water first, then stop watering, and weigh each small pot before stopping watering to ensure that the soil moisture content in each pot is consistent. After treatment for about 20 days, contr...

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Abstract

The invention belongs to the technical field of botany and agriculture, and particularly relates to the application of a plant poly ADP ribose polymerase inhibitor in improving plant traits. The poly ADP ribose polymerase inhibitor comprises 3-AB, BA and the like, and the improvements of plant traits refer to promotion of plant root growth, improvement of tolerance of plants to dry adverse situations and the like. The roots of the plants are developed after the inhibitor is applied for the plants, showing as increase in root and fresh weight increase at a seedling stage. Moreover, the plants also have stronger drought-tolerant capability. The invention can be used for promoting the growth of plant roots and enhancing the drought-tolerant capability of the plants.

Description

Technical field [0001] The invention belongs to the field of plant biology and agricultural technology, and specifically relates to the application of plant poly ADP ribose polymerase inhibitors in improving plant traits. Background technique [0002] Drought is one of the main natural disasters in our country. Drought often leads to a large-scale reduction in crop production and is one of the important environmental factors that limit crop and grain output. In order to reduce the loss of crop yield in drought years, effectively use limited water resources, and improve the drought resistance of crops has always been one of the main goals of agricultural science and technology research. Drought-resistant breeding is currently mainly achieved through hybrid breeding or genetically modified breeding to cultivate drought-resistant strains. At present, my country has invested a lot of money in this area, but the effect is slow, and for each species, due to differences in plant physiol...

Claims

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Application Information

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IPC IPC(8): A01N37/44A01N37/18A01P21/00A01G7/06
Inventor 葛晓春刘伟伟刘财丰张海磊
Owner FUDAN UNIV
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