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A method for extracting and purifying corilagin

An extraction and pure water technology, applied in chemical instruments and methods, organic chemistry, esterification and saccharide, etc., can solve the problems of high cost, low yield and high selectivity, and achieve less impurities, good product quality, and extraction rate. high effect

Inactive Publication Date: 2011-12-21
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese Patent (Application No. 201010535378.3) "A Preparation Method for Curry Latin" discloses that supercritical CO 2 Extraction, macroporous resin adsorption and countercurrent extraction and other technologies combined to obtain Corylatin, this method has high cost, high selectivity and low yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Weigh 2 kg of Phyllostachys phylloxera medicinal material and put it into the extraction equipment, use 18 kg of pure water, heat to 90 ° C, stir and extract for 1 hour, after filtering, add 15 kg of pure water and heat to 90 ° C, stir and extract for 1 hour, filter, and filtrate twice Combined and adsorbed by the pretreated D130 macroporous adsorption resin column, first eluted with water until colorless, and then eluted with 70% methanol, collected the eluate, concentrated under reduced pressure to a small volume, and used an equal volume of the concentrated solution For n-butanol extraction, the extract is concentrated and then input into a supercritical carbon dioxide extraction kettle, under the conditions of extraction temperature 38°C and extraction pressure 28MPa, dynamic continuous extraction for 3 hours, analytical pressure 6MPa, temperature 30°C, CO 2 The flow rate is 22L / h, the entraining agent is 60% acetone solution, and the addition amount is 15% of the me...

Embodiment 2

[0016] Weigh 2kg of Phyllostachys phylloxera medicinal material and put it into the extraction equipment, use 26kg of pure water, heat to 85°C, stir and extract for 1.5 hours, after filtering, add 20kg of pure water and heat to 85°C, stir and extract for 1 hour, filter, and filtrate twice Combined and adsorbed by the pretreated ADS-5 macroporous adsorption resin column, first eluted with water until colorless, and then eluted with 80% methanol, collected the eluate, concentrated it under reduced pressure to a small volume, and used an equal volume of the concentrated solution A large amount of n-butanol was extracted, and the extract was concentrated and then input into a supercritical carbon dioxide extraction kettle. It was dynamically and continuously extracted for 5 hours under the conditions of extraction temperature 42°C and extraction pressure 21.5MPa, analytical pressure 7MPa, temperature 36°C, CO 2 The flow rate is 20L / h, the entrainer is 50% ethanol solution, and the ...

Embodiment 3

[0018] Weigh 2 kg of Phyllostachys phylloxera medicinal material and put it into the extraction equipment, use 30 kg of pure water, heat to 100 ° C, stir and extract for 2 hours, after filtering, add 25 kg of pure water and heat to 100 ° C, stir and extract for 2 hours, filter, and filtrate twice Combined and adsorbed by the pretreated D101 macroporous adsorption resin column, first eluted with water until colorless, then eluted with 65% methanol, collected the eluate, concentrated under reduced pressure to a small volume, and used an equal volume of the concentrated solution For n-butanol extraction, the extract is concentrated and then input into a supercritical carbon dioxide extraction kettle, under the conditions of extraction temperature 31°C and extraction pressure 23MPa, dynamic continuous extraction for 2 hours, analytical pressure 5MPa, temperature 28°C, CO 2 The flow rate is 25L / h, the entraining agent is 70% acetone solution, and the addition amount is 13% of the am...

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Abstract

The invention relates to a method for extracting and purifying corilagin, which comprises: absorbing and analyzing the extract of Phyllanthus phylloxera through macroporous resin, concentrating the desorption liquid and extracting it with n-butanol, and then extracting the extracted liquid with supercritical carbon dioxide fluid to obtain corylatin. Lilatine extract, the extract is further purified by gel column chromatography, and recrystallized to obtain Corilagin. Compared with the prior art, the preparation method has simple operation, low cost and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine chemistry, and relates to a method for extracting and purifying corilagin, in particular to a method for extracting corilagin from Phyllanthus phylloxera by combining techniques such as macroporous resin, supercritical carbon dioxide fluid extraction and gel column chromatography. A method of purifying corilagin. Background technique [0002] Corilagin is a kind of inverse gallic acid tannin, mainly found in plants such as Citrus citrus, Phyllostachys phylloxera, Geranium and other plants, molecular formula: C 27 h 22 o 18 ;Molecular weight: 634.453, white needle crystal powder, easily soluble in methanol, ethanol, Aceton, DMSO, low solubility in cold water. The application history of traditional natural medicines suggests that corilagin has a good preventive effect on chronic cardiovascular diseases. Studies in recent years have shown that corilagin has a certain effect on the preve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H13/08C07H1/08
CPCY02P20/54
Inventor 刘东锋郭琴
Owner NANJING ZELANG MEDICAL TECH
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