The preparation method of soft-shelled turtle peptide

A technology for turtle peptides and turtles, which is applied in the field of biological compound enzyme enzymolysis to prepare turtle peptides, can solve the problems of high cost, salty taste, large molecular weight, and uneven molecular weight distribution, and achieves low cost, no toxic side effects, and simple operation. Effect

Active Publication Date: 2011-12-21
JIANGZHONG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the disadvantages of fishy smell, salty taste, large molecular weight, non-concentrated molecular weight distribution and high cost in the enzymolysis solution of the prior art, the object of the present invention is to provide a method for extracting soft-shelled turtle peptide, using soft-shelled turtle as raw material and using alkaline Turtle peptide obtained by protease or neutral protease enzymolysis and spray drying; this method is easy to operate, has good taste, low cost, environmental protection and high efficiency, and is suitable for industrialized large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] One, preparation method, its steps are as follows:

[0026] 1. Using soft-shelled soft-shelled turtle as raw material, select 100kg of fresh soft-shelled turtle, remove viscera and oil, grind it, add 1000L pure water, and decoct at 100°C for 2 hours;

[0027] 2. Cool to 60°C, add 2kg of alkaline protease at a rate of 2% of the weight of the raw soft-shelled turtle, and enzymatically hydrolyze at a constant temperature of 60°C for 3 hours, or add 1kg of neutral protease at a rate of 1% of the weight of the raw soft-shelled turtle, and heat at 55°C. After 4 hours of hydrolysis, the obtained enzymolysis solution was heated to 100°C, and the enzyme was inactivated for 30 minutes; filtered

[0028] 3. The concentrated density of the filtrate is between 1.05 and 1.1, and it can be spray-dried to obtain 11.64 kg of powdered soft-shelled turtle peptide.

[0029] 2. Determination of Turtle Peptide Content

[0030] 1. method summary

[0031] Low molecular weight protein hydro...

Embodiment 2

[0071] One, the preparation method of soft-shelled turtle peptide, its step is as follows:

[0072] 1. Using Chinese soft-shelled turtle as raw material, select 100kg of fresh soft-shelled turtle, remove viscera, oil, grind, and boil for 1 hour;

[0073] 2. Cool to 40°C, add alkaline protease at a rate of 5% of the weight of the raw soft-shelled turtle, and enzymolyze at a constant temperature of 40°C for 10 hours, or use neutral protease at 4% of the weight of the raw soft-shelled turtle, and enzymolyze at a constant temperature of 40°C for 10 hours. The obtained enzymolysis solution was warmed up to 85°C, and the enzyme was inactivated for 10 minutes;

[0074] 3. Concentrate and spray dry to obtain 12.07kg turtle peptide finished product.

[0075] 2. Determination of soft-shelled turtle peptide content:

[0076] According to the method of Example 1, the molecular weight of soft-shelled turtle peptide is mainly concentrated in 140-2000, accounting for more than 60%.

Embodiment 3

[0078] One, the preparation method of soft-shelled turtle peptide, its step is as follows:

[0079] 1. Using soft-shelled soft-shelled turtle as the raw material, select 100kg of fresh soft-shelled turtle, crush it, and cook for 4 hours;

[0080] 2. Cool to 65°C, add alkaline protease at a rate of 3% of the weight of the raw soft-shelled turtle, and enzymolyze at a constant temperature of 65°C for 2 hours, or use neutral protease at a rate of 3% of the weight of the raw soft-shelled turtle at a constant temperature of 65°C for 4 hours, and The obtained enzymolysis solution was heated to 120°C, and the enzyme was extinguished for 60 minutes;

[0081] 3. Concentrate and spray dry to obtain 13.20kg turtle peptide finished product.

[0082] 2. Determination of soft-shelled turtle peptide content:

[0083] According to the method of Example 1, the molecular weight of soft-shelled turtle peptide is mainly concentrated below 10,000, accounting for more than 99%, of which 140-2000, ...

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Abstract

The invention aims to provide a method for extracting turtle peptide. The method comprises the following steps of: performing enzymolysis on turtle serving as a raw material by using alkali protease or neutral protease for 1 to 10 hours, wherein the alkali protease is 0.2 to 5 percent of the weight of the raw material turtle and the neutral protease is 0.1 to 4 percent of the weight of the raw material turtle; and heating enzymolysis liquid obtained through enzymolysis to the temperature of between 85 and 120DEG C, deactivating enzyme for 10 to 60 minutes, and performing spray drying to obtain the turtle peptide. The method is easy and convenient to implement, ensures good mouthfeel of the turtle peptide, is low in cost, environment-friendly and high-efficiency, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of deep processing of soft-shelled turtles, in particular to a method for preparing soft-shelled turtle peptides by enzymatic hydrolysis of biological compound enzymes using soft-shelled turtles as raw materials. Background technique [0002] Soft-shelled turtle, also known as soft-shelled turtle and soft-shelled turtle, has the effects of nourishing blood, strengthening bones, anti-fatigue, and prolonging life. It is a traditional food in my country and a good raw material for developing high-level health food; Glue, cutin white and multivitamins, etc. Turtle contains high protein, about 165 grams of protein per kilogram, and the protein in food or medicine is generally absorbed and utilized by the body by enzymatic hydrolysis into oligopeptides or amino acids by the digestive juice, so the absorption is slow and the utilization rate is low. Therefore, the desired medicinal effect cannot be achieved. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 钟虹光易敏之卢建中刘根云马莉
Owner JIANGZHONG PHARMA
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