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A pvy/cmv surface plasmon resonance detection method

A surface plasmon and resonance detection technology, which is used in measurement devices, recovery/purification, phase influence characteristic measurement, etc. low cost effect

Inactive Publication Date: 2011-12-28
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved in the present invention is that the detection method of PVY / CMV virus has high cost, low sensitivity, and cannot be quantitatively detected, and a PVY / CMV surface plasmon resonance detection method with low cost, high sensitivity, and quantitative detection can be provided

Method used

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  • A pvy/cmv surface plasmon resonance detection method
  • A pvy/cmv surface plasmon resonance detection method
  • A pvy/cmv surface plasmon resonance detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A PVY surface plasmon resonance detection method is carried out according to the following steps:

[0059] (1) preparing PVY antigen and PVY monoclonal body;

[0060] (2) preparing a biosensor chip;

[0061] a. Chip preparation

[0062] After plating a 40-60nm gold film on a flat film, use Piranha (98% concentrated sulfuric acid and 30% H 2 o 2 According to the mixed solution obtained in the ratio of 7:3) the solution was washed, then rinsed with pure water and absolute ethanol, and dried with nitrogen;

[0063] b. Fixation of basement membrane

[0064] Immerse the chip in 10mM MUOH (11-11-mercapto-1-undecanol) ethanol for 12 to 20 hours, rinse with ultrapure water and absolute ethanol, and blow dry with nitrogen;

[0065] c. Immobilization of linker molecules

[0066] A mixture of equal volumes of 0.4M NaOH and 0.6M epichlorohydrin in diethylene glycol dimethyl ether was used to react with the monolayer MUOH on the chip for 4h to activate the carboxyl group, and ...

Embodiment 2

[0113] In the step a, a 40-45 nm thick gold film is plated on the diaphragm; in the step b, the chip is immersed in ethanol of MUOH for 12-14 hours. Other steps are with embodiment 1.

Embodiment 3

[0115] In the step a, a 45-50 nm thick gold film is plated on the membrane; in the step b, the chip is immersed in MUOH ethanol for 14-16 hours. Other steps are with embodiment 1.

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PUM

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Abstract

The invention discloses a surface plasma resonance detecting method for PVY / CMV (Potato Virus Y / Cucumber Mosaic Virus) and belongs to a material detection method. The surface plasma resonance detecting method comprises the following steps: 1) preparing a PVY / CMV antigen and a monoclonal body; 2) preparing a biosensor chip; 3) preparing a standard curve and acquiring a virus computational formula; and then measuring the content of PVY / CMV by utilizing the virus computational formula. The surface plasma resonance detecting method provided by the invention has high sensitivity, can be used for qualitatively and quantitatively measuring PVY / CMV and is convenient in operation and low in cost.

Description

technical field [0001] The invention belongs to a material detection method, in particular to a PVY / CMV detection method Background technique [0002] CMV (Tobacco Cucumber Mosaic Virus) is a multi-host virus. In addition to infecting tobacco, it also infects potatoes, rapeseed, cucumbers, Henna, eucalyptus, false rye, Sansheng tobacco, quinoa, Burley Tobacco, fine physalis, foreign physalis, heart leaf tobacco, amaranth quinoa and datura and other plants. PVY (Tobacco Potato Virus Y) is a multi-host virus. In addition to infecting tobacco, it also infects potatoes, rapeseed, angelica, eucalyptus, red-billed swallows, false yoghurt, Sansheng tobacco, Burley tobacco, foreign Various plants such as physalis, tobacco, amaranth and datura. Since the discovery of CMV infecting tobacco in my country in 1970s and the discovery of PVY infecting tobacco in my country in 1980s, CMV, PVY and tobacco mosaic virus have infected tobacco together, causing tobacco virus diseases and serio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N21/41C12N7/02C07K16/10
Inventor 刘剑君李富欣邱立友
Owner HENAN AGRICULTURAL UNIVERSITY
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