Glycine Determination Method and Glycine Determination Kit
A technology of glycine transaminase and glycine, which is applied in the field of glycine determination method and its kit, and can solve the problems of not being allowed to use
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Embodiment 1
[0121] Embodiment 1: Determination of glycine content in milk
[0122] 1) Preparation of standard samples
[0123] Dissolve a certain amount of glycine in water or buffer, and then adjust the concentration of glycine to 1 mmol / L;
[0124] 2) Pretreatment of samples to be tested
[0125] The milk sample is used as the sample to be tested without pretreatment;
[0126] 3) Blank sample
[0127] Described water or buffer solution are used as blank sample, and its glycine concentration is 0 mmol / L;
[0128] B. Preparation of reagent solution:
[0129] The glycine determination reagent of the present embodiment is a single-dose reagent, which contains:
[0130] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0131] Glycerin 1mol / L
[0132] NADP + 1.25mmol / L
[0133] Glycine aminotransferase 16000U / L
[0134] Glutamate oxidase 18000U / L
[0135] Isocitrate dehydrogenase 12000U / L
[0136] Ketoglutarate 5mmol / L
[0137] Succinic ac...
Embodiment 2
[0150] Embodiment 2: the mensuration of glycine content in the milk
[0151] 1) Preparation of standard samples
[0152] Dissolve a certain amount of glycine in water, then adjust the concentration of glycine to 1 mmol / L, as a standard sample;
[0153] 2) Preparation of samples to be tested
[0154] The milk sample is used as the sample to be tested and does not need to be processed;
[0155] 3) Blank sample
[0156] Described water is used as blank sample, and its glycine concentration is 0 mmol / L;
[0157] B. Preparation of reagent solution:
[0158] Glycine Assay Reagent is a two-part reagent containing:
[0159] Reagent 1 with the following composition:
[0160] Phosphate buffer 100mmol / L
[0161] NAD + 2.25mmol / L
[0162] Ketoglutarate 5mmol / L
[0163] Succinic acid 15mmol / L;
[0164] Reagent 2 with the following composition:
[0165] Phosphate buffer 100mmol / L
[0166] Ethylene glycol 5mol / L
[0167] Glycine aminotransferase ...
Embodiment 3
[0186] Embodiment 3: the mensuration of glycine content in the milk sample
[0187] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:
[0188] B. Preparation of reagent solution:
[0189] Glycine Assay Reagent is a three-part reagent that contains:
[0190] Reagent 1 with the following composition:
[0191] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0192] NAD + 2.25mmol / L
[0193] Ketoglutarate 6mmol / L
[0194] Succinic acid 14mmol / L;
[0195] Reagent 2 with the following composition:
[0196] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0197] Ammonium sulfate 1mol / L
[0198] Glutamate oxidase 28000U / L
[0199] Isocitrate dehydrogenase 50000U / L;
[0200] Reagent 3 with the following composition:
[0201] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0202] Ammonium sulfate 2mol / L
[0203] Glycine aminotransferase 26000U / ...
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