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Culture method for chlorella

A cultivation method and technology of chlorella, applied in the field of cultivation of chlorella, can solve the problems of affecting the growth rate of microalgae and the inability of microalgae to grow, so as to increase the accumulation amount, avoid the control of temperature and improve the utilization rate Effect

Active Publication Date: 2012-01-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reduction in temperature will seriously affect the growth rate of microalgae, and even make most of the microalgae unable to grow. Therefore, there are few reports about cultivating microalgae at low temperatures in the prior art.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In the bioreactor, the volume of the chlorella algae seed solution accounted for 10% of the total volume of the medium, the stirring speed was 90rpm, the light intensity was 30001x, and CO 2 The gas is the tail gas emitted by anaerobic fermentation or microaerobic fermentation process, CO 2 The gas content is 10v%, the ventilation rate is 0.5vvm, the pH is 7-10 during the culture period, the culture temperature is reduced from 30°C to 5°C every day, and the constant temperature is cultivated for 1 day for every 5°C drop until the temperature drops to 0°C. Continue constant temperature cultivation at 0°C for 10 days. A number of single algae colonies are obtained. After picking the single algae colonies, they are cultured in the solid slant basic medium. After the growth, the pure algae species FY1T can be obtained. Transfer the pure Chlorella species from the inclined plane to a shake flask for cultivation under the conditions of 5-15°C, 100rpm, 30001x light and an air...

Embodiment 2

[0025] In the bioreactor, the volume of the chlorella algae seed solution accounted for 15% of the total volume of the medium, the stirring speed was 100rpm, the light intensity was 20001x, and CO 2 The gas is the gas after deoxygenation treatment of the tail gas in the aerobic microbial fermentation process, the oxygen content is less than 8v%, CO 2 The gas content is 5v%, the ventilation rate is 1.0vvm, the pH is 7-10 during the culture period, the culture temperature is uniformly cooled to 0°C within 6 days from 25°C, and the constant temperature culture is continued at 0°C for 10 days. A number of single algae colonies are obtained. After picking the single algae colonies, they are cultured in the solid slant basic medium. After the growth, the pure algae species FY1T can be obtained. Transfer the pure Chlorella species from the inclined plane to a shake flask for cultivation under the conditions of 5-15°C, 100rpm, 30001x light and an air bath, and obtain experimental data...

Embodiment 3

[0027] In the bioreactor, the volume of the chlorella algae seed solution accounts for 5% of the total volume of the medium, and 0.02mmol / L of Na is added to the medium. 2 SO 3 and 0.8mL / L trace element solution, the composition of the above-mentioned trace element mixed solution per liter is as follows: H 3 BO 3 6.0g, ZnSO 4 ·7H 2 O 4.5g, MnCl2 ·H 2 O 0.75g, (NH 4 ) 6 Mo 7 o 24 4H 2 O 0.35g, CuSO 4 ·5H 2 O 0.75g, Co(NO 3 ) 2 ·6H 2 O 0.15g, the stirring speed is 80rpm, the light intensity is 30001x, and the 2 The gas is the tail gas emitted by anaerobic fermentation or microaerobic fermentation process, CO 2 The gas content is 15v%, the ventilation rate is 0.1vvm, the pH is 7-10 during the culture period, the culture temperature is uniformly cooled from 35°C to 10°C within 5 days, and the constant temperature culture is continued at 10°C for 10 days. A number of single algae colonies are obtained. After picking the single algae colonies, they are cultured in t...

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Abstract

The invention discloses a culture method for chlorella, which comprises the following contents: the volume of chlorella seed liquid in a bioreactor accounts for 5 to 15 percent of the total volume of a culture medium; a gas containing 5 to 15 volume percent of CO2 is introduced, the volume of introduced gas is 0.1 to 1.0vvm; and the culture temperature is reduced to 0 to 15 DEG C from 25 to 40 DEG C within 0.5 to 10 days, and chlorella is continuously cultured at a constant temperature of 0 to 15 DEG C for 5 to 15 days. The method disclosed by the invention can allow microalgae to grow normally at a low temperature, and the accumulated amount of fat and CO2 utilization rate of microalgae can be increased.

Description

technical field [0001] The invention belongs to the technical field of microalgae, and relates to a method for cultivating chlorella. Background technique [0002] Microalgae are rich in protein, polysaccharides, unsaturated fatty acids and other nutrients (such as spirulina), which can be used in food, medicine and energy; they can accumulate fatty acids in large quantities, and the fatty acid content of some microalgae can account for 30% to 60% of dry weight. The use of microalgae to accumulate oil resources has become the most popular research field in the development of renewable resources using solar energy. Not only has strong market potential, but also has extraordinary social value. [0003] However, most wild microalgae grow slowly and have limited ability to fix carbon dioxide. To realize large-scale production, it needs to consume a lot of manpower and material resources, but the harvest is very small. At present, microalgae research is in a hot period, and the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王领民金平师文静李晓姝王崇辉佟明友黎元生
Owner CHINA PETROLEUM & CHEM CORP
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