PCR primer pair for identification or auxiliary identification of tissues and/or organs of mouse and applications thereof

Abstract

The invention discloses a PCR primer pair for identification or auxiliary identification of tissues and / or organs of a mouse and applications thereof. The primer pair provided by the invention comprises at least one primer pair of the PCR primer pair for identification or auxiliary identification of the tissues and / or organs of the mouse which is comprises in six PCR primer pairs for identification or auxiliary identification of tissues and / or organs of the mouse, a cattle, a sheep, a pig, a chicken and a duck. The six PCR primer pairs for identification or auxiliary identification of the tissues and / or organs of the mouse, the cattle, the sheep, the pig, the chicken and the duck respectively and successively comprise two single chains represented by SEQ ID NO: 1 and SEQ ID NO: 2, two single chains represented by SEQ ID NO: 3 and SEQ ID NO: 4, two single chains represented by SEQ ID NO: 5 and SEQ ID NO: 6, two single chains represented by SEQ ID NO: 7 and SEQ ID NO: 8, two single chains represented by SEQ ID NO: 9 and SEQ ID NO: 10, and two single chains represented by SEQ ID NO: 11 and SEQ ID NO: 12. The primer pairs in the invention select an annealing temperature of 62DEG C, soall discriminations with one PCR at a same temperature are realized, the specificity is strong, the time consumption of the detection process is short.

Description

technical field [0001] The present invention relates to a pair of PCR primers for identifying or assisting identification of animal tissues and / or organs and applications thereof, in particular to the tissue that can identify or assist identification of at least one of the six animals of mouse, cow, sheep, pig, chicken and duck and / or PCR primer pairs for organs. Background technique [0002] At present, in the production and sales of domestic raw meat and meat products, in order to make huge profits, some unscrupulous traders use adulteration and other means to deceive consumers from time to time, such as using rat meat as pork, beef and mutton. Therefore, a set of methods for quickly and accurately identifying meat types and being able to adapt to the identification of mixed meat products will undoubtedly provide strong technical support for law enforcement officers. [0003] The traditional identification methods of meat products are mainly based on protein level analysi...

AI Technical Summary

Problems solved by technology

This type of identification technology is greatly affected by the structure of the protein (the part that plays a major role is the antigenic determinant), and the problems that often occur in practical applications include: 1. The thermal processing process will change the structure of the protein (the antigenic determinant). Detect heat-processed meat; 2. Cross-reaction may occur in the detection of mixed meat, because the structure of proteins of different species may have a certain degree of similarity, and the sensitivity of the test depends on the specificity of the prepared antibody

The DNA molecular hybridization method is complicated to operate, and the cost is high, so it is gradually replaced by the PCR method

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