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Preparation method of citric acid fermentation broth

A citric acid fermentation broth, citric acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of prolonged fermentation production cycle, difficult citric acid acidity, polluted environmental wastewater, etc., and achieve sugar consumption Small size, lower electricity cost, lower COD effect

Inactive Publication Date: 2012-01-18
COFCO BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this method will cause waste of water resources, more importantly, in order to control the content of residual reducing sugar as low as possible, it will cause the fermentation production cycle to prolong, the problem of high energy consumption, and also will produce a large amount of waste water polluting the environment, in addition , there are also certain difficulties in increasing the acidity of citric acid in the fermentation broth

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0011] According to the preparation method of citric acid fermented liquid provided by the present invention, wherein, in the pulping step, the pulping liquid is an aqueous solution containing citric acid, and the aqueous solution containing citric acid contains Neutralization waste liquid generated during the purification process of citric acid fermentation liquid. The use of an aqueous solution containing citric acid as the slurry can save the original amount of water used for slurrying, to achieve the purpose of recycling waste water and saving water resources, and more importantly, the process of purifying the citric acid fermentation liquid obtained in the fermentation step The neutralization waste liquid produced in the process also contains some reducing sugars. Therefore, on the one hand, the part of the unfermented reducing sugar can be mixed with the starchy raw material for further enzymolysis and fermentation through the reuse of wastewater, so that there is no nee...

Embodiment 1

[0050] This example is used to illustrate the preparation of the citric acid fermentation broth provided by the present invention.

[0051] 1) Grinding of raw materials: put the harvested corn in a hot water tank until the water content of the corn is 15% by weight, and then grind it through a pulverizer (Jiangsu Muyang Group Co., Ltd., 968-3 type) to obtain a starchy raw material. Product (50% by weight of the crushed particles has a particle diameter of less than 0.1 cm);

[0052] 2) sizing: use the neutralization waste liquid from step 8) (the content of citric acid in the aqueous solution in 1 liter of neutralization wastewater is 2.2 grams) as the sizing liquid and the starchy raw material to mix and carry out the sizing to obtain the starch slurry, the The weight ratio of the starchy raw material to the sizing liquid is 1: 2.57, and the pH value of the starch sizing liquid is 5.5;

[0053] 3) Enzymolysis: the starch slurry obtained in 2) is mixed with amylase (Novozymes...

Embodiment 2

[0060] This example is used to illustrate the preparation of the citric acid fermentation broth provided by the present invention.

[0061] 1) Peeling and crushing of raw materials: put the harvested corn in a hot water tank and stew until the water content of the corn is 15% by weight, and then crush it with a grinder (Jiangsu Muyang Group Co., Ltd., 968-3 type) to obtain starch High-quality raw materials (52% by weight of the crushed particles have a particle diameter less than 0.1 cm);

[0062] 2) pulping: use the neutralizing waste liquid from step 8) (the content of citric acid in the aqueous solution in 1 liter of neutralizing waste water is 2.3 grams) and raw water to deploy the pulping liquid, the consumption of the raw water accounts for 50% by weight of the pulping liquid, Mixing the sizing liquid and the starchy raw material for sizing to obtain a starch slurry, the weight ratio of the starchy raw material to the sizing liquid is 1:2.3, and the pH value of the starch ...

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Abstract

The invention provides a preparation method of citric acid fermentation broth, and the method comprises: a slurry blending step, that is, mixing slurry-blending liquid and a starchiness raw material to obtain starch slurry, wherein the slurry-blending liquid is an aqueous solution containing citric acid which comprises neutralization waste liquid generated in the purification process of citric acid fermentation broth from a fermentation step, and the weight ratio of the starchiness raw material and the slurry-blending liquid is 1:1.86-3; an enzymolysis step, that is, mixing the starch slurry obtained in the slurry blending step with enzymes and performing enzymolysis under an enzymolytic condition to obtain an enzymolytic product; a fermentation step, that is, fermenting the enzymolytic product obtained in the enzymolysis step under a condition for citric acid generation so as to obtain the citric acid fermentation broth, wherein the fermentation endpoint is that the residual reducing sugar content of the fermentation broth is 1-10 g / L. The method of the invention can greatly reduce process water consumption, shorten production period, increase the production efficiency of citric acid, reach the purpose of energy saving, and obtain high-concentration citric acid fermentation broth.

Description

field of invention [0001] The invention relates to a preparation method of citric acid fermentation broth. Background of the invention [0002] Citric acid is an important industrial organic acid, known as the first edible sour agent, widely used in the food industry, and also widely used in medicine, beauty, cosmetics, detergents, and industrial fields. Since the Swedish chemist Scheel first extracted citric acid from lemon juice and crystallized solid citric acid in 1784, after continuous research and exploration by foreign scientists, he realized the industrialization of submerged fermentation, thus realizing the large-scale production of citric acid. [0003] my country's citric acid industry started in the 1950s. Through the joint efforts of several generations, the technical level of citric acid has been in the leading position in the world, and the rapid development of citric acid industry has been realized. The preparation of citric acid generally includes: pretreat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/48C12R1/685
Inventor 陈修李东栋熊结青卢宗梅廖四祥
Owner COFCO BIOTECHNOLOGY CO LTD
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