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Preparation method for tubular silk fibroin scaffold for tissue repair

A technology for silk fibroin and tissue repair, applied in the field of preparation of silk fibroin tubular scaffolds, can solve the problems of low tensile strength and elongation at break, poor biomechanical properties, etc., achieve good biocompatibility, improve The effect of biomechanical properties

Active Publication Date: 2014-07-16
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The prior art has disclosed a variety of methods for preparing tubular tissue repair scaffolds using silkworm silk fibroin as raw material. For example, the Kaplan research group in the United States dissolved silk fibroin and directly formed it into a tube, or formed it into a tube by electrospinning, or sprayed it into a tube by electrostatic spraying. After forming a tubular shape, the tubular material is freeze-dried to make a small-diameter blood vessel repair material (Biomaterials, 2007 (28): 5271-5279; Biomaterials, 2008 (29): 4650-4657; Biomaterials, 2008 (29): 2217-2227 ; Biomaterials, 2009 (30): 3213-3223); Domestic also discloses the method (International Journal of Biological Macromolecules, 2009 (45): 504-510) of preparing silk fibroin monolayer tubular material by electrospinning, but present The silk fibroin tubular scaffolds are all composed of silk fibroin monolayer material, which has poor biomechanical properties, low tensile strength and elongation at break.

Method used

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  • Preparation method for tubular silk fibroin scaffold for tissue repair

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preparation example Construction

[0023] The invention provides a method for preparing a silk fibroin tubular scaffold for tissue repair, comprising:

[0024] The tubular fabric woven into silkworm boiled silk thread is loaded on the mould;

[0025] Adding a hydrophilic adhesive to the silkworm silk fibroin solution to obtain a mixed solution;

[0026] The mold equipped with the tubular fabric is soaked in the mixed solution, and the mold is taken out after freezing treatment to obtain a silk fibroin tubular support for tissue repair.

[0027] The invention uses silkworm silk fibroin and boiled silkworm thread as raw materials to prepare silk fibroin tubular scaffolds, and the silk fibroin tubular scaffolds have good biocompatibility and biomechanical properties.

[0028] The invention uses the boiled silk thread of silkworm as a raw material, and weaves it into a tubular fabric. In order to make the obtained silk fibroin tubular stent more suitable for use as an artificial blood vessel, the inner diameter o...

Embodiment 1

[0049] Weaving the cooked silk thread of silkworm with a specification of 16×20 / 22 into a tubular fabric with an inner diameter of 2 mm and a thickness of 0.6 mm by weft knitting;

[0050] Boil 0.2kg silkworm silk in 10L mass concentration of 0.25% sodium carbonate tap water solution for 30min, 10L mass concentration of 0.25% sodium carbonate tap water solution for 30min and 10L mass concentration of 0.25% sodium carbonate deionized aqueous solution Boil for 45 minutes; wash and loosen the obtained silk, and dry it in an oven at 60°C to obtain silkworm silk fibroin;

[0051] Dissolve 10 g of silkworm silk fibroin in 100 mL of an aqueous solution of calcium chloride and ethanol at a molar ratio of 1:2, stir and dissolve at 70°C for 2 hours to obtain the first solution;

[0052] The first solution is poured into the dialysis bag and then soaked in deionized water, the deionized water is replaced every 2 hours, and the dialysis is continued for 4 days to obtain a silkworm fibroin...

Embodiment 2

[0060] Weaving the cooked silk thread of silkworm with a specification of 12×20 / 22 into a tubular fabric with an inner diameter of 3mm and a thickness of 0.5mm by weft knitting;

[0061] Boil 0.2kg silkworm silk in 10L mass concentration of 0.25% sodium carbonate tap water solution for 30min, 10L mass concentration of 0.25% sodium carbonate tap water solution for 30min and 10L mass concentration of 0.25% sodium carbonate deionized aqueous solution Boil for 45 minutes; wash and loosen the obtained silk, and dry it in an oven at 60°C to obtain silkworm silk fibroin;

[0062] Dissolve 10 g of silkworm silk fibroin in 100 mL of an aqueous solution of calcium chloride and ethanol at a molar ratio of 1:2, stir and dissolve at 70°C for 2 hours to obtain the first solution;

[0063] The first solution is poured into the dialysis bag and then soaked in deionized water, the deionized water is replaced every 2 hours, and the dialysis is continued for 4 days to obtain a silkworm fibroin p...

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Abstract

The present invention provides a preparation method for a tubular silk fibroin scaffold for tissue repair. The method comprises: filling tubular fabric in a mold, wherein the tubular fabric is woven from cooked silkworm silk; adding a hydrophilic adhesive to a silkworm silk fibroin solution to obtain a mixed solution; immersing the mold filled with the tubular fabric in the mixed solution; carrying out a freezing treatment and removing the mold to obtain the tubular silk fibroin scaffold for the tissue repair. The tubular fabric provides a support layer effect in the tubular silk fibroin scaffold, such that the biomechanical performance of the tubular silk fibroin scaffold can be improved. The silk fibroin has good biocompatibility, such that the tubular silk fibroin scaffold has good biocompatibility. Therefore, the tubular silk fibroin scaffold provided by the present invention has good biocompatibility and good biomechanical performance. The experimental results show that: the tensile strength of the tubular silk fibroin scaffold provided by the present invention is more than or equal to 600 kPa, the elongation at break of the tubular silk fibroin scaffold provided by the present invention is more than or equal to 20%.

Description

technical field [0001] The invention relates to the technical field of tissue repair materials, in particular to a method for preparing a silk fibroin tubular support for tissue repair. Background technique [0002] In the field of regenerative medicine and tissue engineering, tubular scaffolds can be used as repair materials for various organs and tissue injuries, such as artificial nerve tubes, artificial trachea, artificial blood vessels, etc. At present, the tubular stents used in medicine for artificial blood vessels are mainly made of polyurethane fibers, polyester or expanded polytetrafluoroethylene. However, polyurethane fibers are prone to thrombosis and have poor long-term patency; polyester and expanded polytetrafluoroethylene Ethylene has strong hydrophobicity and poor biocompatibility, which will affect the healing of organs or tissues. Therefore, materials with better biocompatibility as tubular scaffolds have gradually become a research hotspot. [0003] Bomb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/50
Inventor 王建南刘志武胡震宇杜大方
Owner SUZHOU UNIV