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Mesenchymal stem cell self-preserving liquid

A stem cell and autologous technology, applied in the field of mesenchymal stem cell autologous preservation solution, can solve the problems of affecting clinical effect, stem cell death, low cell viability, etc., and achieve the effect of long-term transportation preservation

Active Publication Date: 2013-04-03
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing stem cell preservation solution is mainly 0.9% sodium chloride injection, and other improvements include glucose sodium chloride injection, etc. These protective solutions are safe for human intravenous reinfusion, but due to osmotic pressure and other reasons A large number of stem cells die, and the cell viability is very low after long-term transportation, which directly affects the clinical effect

Method used

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  • Mesenchymal stem cell self-preserving liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028]

[0029] 1.1 Operation steps:

[0030] 1.1.1 Preservation solution 1 was prepared by autologous platelet lysate, mixed sugar-electrolyte injection, and vitamin C according to the above ratio; preservative solution 2 was prepared by autologous platelet lysate, normal saline injection, and vitamin C injection according to the above ratio;

[0031] 1.1.2 Prepare adipose-derived mesenchymal stem cell suspensions with preservation solution 1 and preservation solution 2 respectively, and put them into 2ml cryopreservation tubes, in which the number of adipose-derived mesenchymal stem cells is 3×10 6 / ml; prepare 9 tubes for each, 3 tubes as a group, set up 6 groups and store at 4°C for 8h, 12h, 24h;

[0032] 1.1.3 The cells were counted with the automatic cell counter Countess of Invitrogen Company. The results of each group were averaged as described in Table 1.

[0033] Table 1: Detection results of cell viability of adipose-derived mesenchymal stem cells in preservati...

Embodiment 2

[0037] 2.1 Operation steps:

[0038] 2.1.1 The autologous platelet lysate with a volume ratio of 1%, 2%, 3%, 4%, 5%, and 6%, vitamin C injection with a volume ratio of 1%, and the rest mixed sugar electrolyte injection were prepared preservation solution;

[0039] 2.1.2 Prepare adipose-derived mesenchymal stem cell suspensions with different volume ratios of autologous platelet lysate preservation solutions, put them into 2ml cryopreservation tubes, and the number of adipose-derived mesenchymal stem cells is 3×10 6 / ml; prepare 6 tubes for each, 3 tubes as a group, set up 12 groups and store at 4°C for 8h and 12h respectively;

[0040] 2.1.3 The cells were counted with the automatic cell counter Countess of Invitrogen Company. The results of each group were averaged as described in Table 2.

[0041] Table 2: Detection results of adipose-derived mesenchymal stem cells over time in preservation solutions of autologous platelet lysate at different volume ratios

[0042]

...

Embodiment 3

[0045]

[0046]

[0047] 3.1 Operation steps:

[0048] 3.1.1 Preservation solution 3 was prepared by autologous platelet lysate, mixed sugar electrolyte injection and vitamin C according to the above ratio; preservation solution 4 was prepared by autologous platelet lysate and mixed sugar electrolyte injection according to the above ratio;

[0049] 3.1.2 Prepare adipose-derived mesenchymal stem cell suspensions with preservation solution 3 and preservation solution 4 respectively, and put them into 2ml cryopreservation tubes, in which the number of adipose-derived mesenchymal stem cells is 3×10 6 / ml; prepare 9 tubes for each, 3 tubes as a group, set up 6 groups and store at 4°C for 8h, 12h, 24h;

[0050] 3.1.3 The cells were counted with the automatic cell counter Countess of Invitrogen Company. The average results of each group are shown in Table 3.

[0051] Table 3: Detection results of cell viability of adipose-derived mesenchymal stem cells in preservation solutio...

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Abstract

The invention discloses a mesenchymal stem cell self-preserving liquid which is prepared from the following raw materials according to volume ratios: 1-6% of self platelet lysate, 95-97% of solution medium and 0-1% of vitamin C injection, wherein the solution medium is a mixed sugar electrolyte injection or physiological saline injection. The mesenchymal stem cell self-preserving liquid has the advantages that: growth factors contained in the mesenchymal stem cell self-preserving liquid can maintain the activity state of cells, the vitamin C can maintain the activity of various peroxidases, at the same time, the mixed sugar electrolyte injection is especially suitable for cell preservation, and osmotic pressure is also favorable for nutrient absorption of the cells so as to carry out metabolism; the activity of the mesenchymal stem cells which are preserved in the mesenchymal stem cell self-preserving liquid within 8 hours is still larger than 90%, the cell activity change is not larger than 7%, and the activity of the mesenchymal stem cells which are preserved in the mesenchymal stem cell self-preserving liquid within 12 hours is still maintained at 85%; and by using the mesenchymal stem cell self-preserving liquid, the requirements for human intravenous back-transfusion safety can be met, and the cell preserving problem of long-time transport is solved, thus more patients have opportunity to undergo mesenchymal stem cell treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an autologous preservation solution of mesenchymal stem cells. Background technique [0002] Mesenchymal Stem Cells (MSCs) are an important member of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development, and have the characteristics of multi-directional differentiation potential, hematopoietic support and promotion of stem cell implantation, immune regulation and self-replication. And increasingly people's attention. Mesenchymal stem cells are widely present in various tissues throughout the body, cultured and expanded in vitro, and can differentiate into cardiomyocytes, nerve cells, and fat cells under specific conditions. With the in-depth research on the biological characteristics and functions of mesenchymal stem cells by scholars at home and abroad, mesenchymal stem cells can be isolated from various tissues. Mesenchymal s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 申重阳郑榆坤海泉王翔张校通
Owner CHENGDU QINGKE BIOTECH
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