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Method for removing nitrite in edible bird's nest by using microbial viable bacteria suspension and/or crude enzyme liquid and application

A technology of nitrite and crude enzyme liquid, applied in application, food science, food preparation and other directions, can solve the problem of removing nitrite in bird's nest without biological method, and achieve the effect of convenient storage and use

Active Publication Date: 2012-02-29
邓毛程 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no relevant report on the removal of nitrite in bird's nest by biological methods

Method used

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  • Method for removing nitrite in edible bird's nest by using microbial viable bacteria suspension and/or crude enzyme liquid and application
  • Method for removing nitrite in edible bird's nest by using microbial viable bacteria suspension and/or crude enzyme liquid and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Use microbial viable bacteria suspension to remove nitrite in bird's nest, the process is as follows figure 1 shown:

[0054] (1) Preparation of culture medium

[0055] Adjust 200 mL of wort (10°Brix) to pH 4.5, put it into a 1000 mL conical flask, wrap the mouth of the bottle with gauze, sterilize at 125°C for 10 min, and cool it for later use.

[0056] (2) Preparation of viable bacterial suspension by direct culture

[0057] The slanted strain of Rhododendron viscera CICC31229 (purchased from the China Industrial Microorganism Culture Collection Center) was inserted into the culture medium, and the inoculation amount was 2 rings (the diameter of the inoculation ring was 0.6 mm), and it was placed at 28 ° C and cultured at 150 r / min for 24 h to obtain yeast. The number is 2.57×10 7 cells / mL of viable bacterial suspension.

[0058] (3) Removal of nitrite in bird's nest

[0059] 2g bird's nest (commercially available, originating in Malaysia, adopt acid hydrolysis p...

Embodiment 2

[0061] Use microbial viable bacteria suspension to remove nitrite in bird's nest, the process is as follows figure 1 shown:

[0062] (1) Preparation of culture medium

[0063] 200 mL of wort (12°Brix) was adjusted to pH 4.0, put into a 1000mL conical flask, the mouth of the bottle was wrapped with gauze, sterilized at 125°C for 20min, and then used after cooling.

[0064] (2) Preparation of viable bacterial suspension by direct culture

[0065] Crimson yeast CICC32918 (purchased from the China Industrial Microbial Culture Collection Center) slanted strain was inserted into the medium, and the inoculation amount was 4 rings (the diameter of the inoculation ring was 0.6 mm), and it was placed at 28 ° C and cultured at 150 r / min for 24 h to obtain yeast. The number is 1.97×10 7 cells / mL of viable bacterial suspension.

[0066] (3) Removal of nitrite in bird's nest

[0067] 10g bird's nest (commercially available, originating in Malaysia, adopt acid hydrolysis processing samp...

Embodiment 3

[0069] Use microbial viable bacteria suspension to remove nitrite in bird's nest, the process is as follows figure 1 shown:

[0070] (1) Preparation of culture medium

[0071] 2970mL of wort (12°Brix) was adjusted to pH 4.0, put into a 5L fermenter, sterilized at 121°C for 15min, and cooled for later use.

[0072] (2) Preparation of live bacterial suspension from active dry yeast

[0073] Shake flask seed culture: adjust 100mL wort (12°Brix) to pH 4.0, put into 500mL conical flask, wrap with gauze, sterilize at 125°C for 10min, after cooling, insert 1 ring (inoculation ring diameter 0.6mm) sticky red Yeast CICC31229 (purchased from China Industrial Microorganism Culture Collection Center) slanted strain, placed at 30° C., 200 r / min and cultured for 20 h, to obtain Rhododendron viscera shake flask seed liquid.

[0074] Fermentation tank culture: connect 30 mL of Rhodotorula rubrum seed liquid into the fermentation tank medium, the culture temperature is controlled at 30 °C, ...

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Abstract

The invention discloses a method for removing nitrite in an edible bird's nest by using microbial viable bacteria suspension and / or crude enzyme liquid and application. The method is characterized in that one or two of the microbial viable bacteria suspension which is prepared by using edible microbes with an ability of removing the nitride and the crude enzyme liquid which is prepared by using the edible microbes with the ability of removing the nitride is mixed with the edible bird's nest, and then the edible bird's nest is oscillated and washed to obtain the edible bird's nest in which thenitride is removed. The microbial viable bacteria suspension is obtained directly through fermentation or by suspending active dry yeasts in a culture medium. The crude enzyme liquid is obtained through thallus crushing and separation or by dissolving dry crude enzyme powder in buffer solution. The active dry yeasts and the dry crude enzyme powder are convenient to store and use and can be used both during food production and before eating. By using the microbes with edible safety and the crude enzyme liquid thereof to remove the nitride in the edible bird's nest, the removal rate can reach more than 99 percent. The method is safe and high-efficiency and can also be used for removing the nitride in other cured foods.

Description

technical field [0001] The invention relates to a method for removing nitrite in bird's nest, in particular to a method and application for removing nitrite in bird's nest by utilizing viable microorganism suspension and / or crude enzyme liquid, and belongs to the field of biotechnology. Background technique [0002] Bird's nest is a nest formed by bonding the saliva secreted by a variety of birds of the Swift family (Apodidae) swiftlet genus (Collocalia) with down feathers, weeds, etc. Commercial bird's nest. Modern research shows that bird's nest can not only be used as a tonic to supplement protein and amino acids, but also contains some active proteins, which can inhibit blood coagulation, promote cell division, delay brain tissue aging, and improve immunity. Therefore, bird’s nest is a well-known high-grade tonic for both medicinal and edible purposes. It is often used to invigorate the lungs and nourish yin, invigorate the middle and replenish qi, moisten dryness and d...

Claims

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Application Information

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IPC IPC(8): A23L1/015A23L5/20
Inventor 邓毛程李静王瑶顾宗珠朱晓立廖帆余运红杨旭霞徐伟强蔡晓妮黄崇俊
Owner 邓毛程
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