Malt root resource comprehensive utilization method
A malt root and recycling technology, which is applied in the field of comprehensive utilization of malt root resources, can solve the problems of high cost of small-scale extraction and insufficient utilization of nutrients, and achieve the goal of achieving harmlessness, reducing waste of resources, and reducing costs Effect
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Embodiment 1
[0030] The first step, the preparation of malt root protein:
[0031] Accurately weigh barley root 10g, pulverize the powder through a 50 mesh sieve, then add 4 times the volume of deionized water, add 4mol / L phosphoric acid after standing for 10min to adjust pH 6.0, add 0.01% (w / w) compound enzyme ( Cellulase: xylanase: β-glucanase = 1:1:1), enzymolysis at 30°C for 60 minutes, then the mixture was filtered to remove undecomposed residue, and the filtrate was subjected to 110°C, After 10 minutes of denaturation treatment, after cooling to room temperature, the flocculin was obtained by filtration, and the filtrate was used in the second step for later use. The above-mentioned flocculents were washed three times with deionized water to remove soluble impurities, filtered and dried to obtain barley root protein dry powder, and the final yield of barley root protein was 16.4%. V = 78.6 mL, Vo = 10.1 mL.
[0032] The second step, the preparation of active yeast powder:
[0033]...
Embodiment 2
[0035] The first step, the preparation of malt root protein:
[0036]Accurately weigh barley root 10g, pulverize the powder through a 70 mesh sieve, then add 4 times the volume of deionized water, add 4mol / L phosphoric acid after standing for 10min to adjust the pH to 5.5, add 0.01% (w / w) compound enzyme ( Cellulase: xylanase: β-glucanase = 1:1:1), enzymolysis at 30°C for 90 minutes, then the mixture was filtered to remove undecomposed residue, and the filtrate was subjected to 110°C, Denaturation treatment for 10 minutes, cooling to room temperature and filtration to obtain flocculin, the filtrate is used in step 2 for later use. The above-mentioned flocculin was washed three times with deionized water to remove soluble impurities, and filtered and dried to obtain barley root protein dry powder. The final yield of barley root protein was 17.3%. V=79.3mL, Vo=9.9mL
[0037] The second step, the preparation of active yeast powder:
[0038] Take the filtrate obtained by filter...
Embodiment 3
[0039] Embodiment 3, small-scale scale-up experiment
[0040] The first step, the preparation of malt root protein:
[0041] Accurately weigh barley root 100Kg, pulverize the powder through 80 mesh sieves, then add 4 times the volume of deionized water, add 4mol / L phosphoric acid after standing for 10min to adjust pH 6.0, add 0.01% (w / w) compound enzyme ( Cellulase: xylanase: β-glucanase = 1:1:1), enzymolysis at 30°C for 90 minutes, then the mixture was filtered to remove undecomposed residue, and the filtrate was subjected to 110°C, After 10 minutes of denaturation treatment, after cooling to room temperature, the flocculin was obtained by filtration, and the filtrate was used in the second step for later use. The above-mentioned flocculin was washed three times with deionized water to remove soluble impurities, and filtered and dried to obtain barley root protein dry powder. The final yield of barley root protein was 17.2%. V=79.0mL, Vo=10mL
[0042] The second step, the ...
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