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Peach bacterial canker diagnosis method

A technology of peach canker and diagnosis method, which is applied in the field of rapid identification of peach canker diagnosis, and can solve the problems that it is not suitable for general promotion.

Inactive Publication Date: 2013-08-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Claims
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Problems solved by technology

Although these methods are reliable, they can only be operated in the laboratory and are not suitable for general promotion. In addition, these techniques require professional operation skills and background

Method used

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  • Peach bacterial canker diagnosis method
  • Peach bacterial canker diagnosis method
  • Peach bacterial canker diagnosis method

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Experimental program
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Embodiment Construction

[0012] 1. Materials and methods

[0013] Potassium sulfate, potassium chloride, sodium chloride, potassium dichromate, magnesium chloride. All reagents were of analytical grade and purchased from Aladdin Reagent Company. The peach branch was the diseased branch of the year in the field, and a 5 cm branch was cut off, and densely grown small black spots could be seen on the branch (the branch was determined to be the diseased branch of peach canker by conventional methods). Simultaneously also prepare the branches of other diseases on some peach trees (this branch is determined not to be the morbidity branch of peach tree canker by routine method), as a control test.

[0014] Methods The saturated salt solution standard humidity field was used to measure the conidia secretion of these infected peach branches under different humidity and different temperatures. Refer to the values ​​in Table 1 for the fixed point data of the standard humidity of the saturated salt solution.

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Abstract

The invention provides a peach bacterial canker diagnosis method, which is characterized in that the method comprises the following steps: (1), taking diseased branches with visible dense small black spots and cutting out 5-8cm; (2), cultivating the peach branches at the humidity larger than 75% and smaller than 10% and the temperature higher than 10 DEG C and lower than 40 DEG C for at least oneday; and (3), determining the peach bacterial canker if conidium liquid appears on the small black spots on the peach branches. Whether the peach branches are infected with the bacterial canker can be diagnosed easily and quickly by the method, and expensive laboratory equipment and professional technical backgrounds are not needed.

Description

technical field [0001] The invention belongs to a method for diagnosing fruit tree diseases, in particular to a method for quickly identifying peach tree canker. Background technique [0002] Peach( Prunus persica (L.) Batsch var. nucipersica ) belongs to the subgenus Prunus in the Rosaceae family. It is native to Shaanxi, my country, and has a cultivation history of more than 3,000 years. [0003] Peach branch blight is caused by the fungus Phomopsis spp. Phomopsis amygdali , Original Fusicoccum amygdali ), the pathogen was inspected and quarantined in Zhejiang Province before 1997, generally known as peach canker. The disease enters through the lenticels or flower buds, and mainly harms the branches of peach trees, forming a ring-shaped slightly sunken brown withered spot, and the branches and leaves quickly wither and turn yellow. Since 2009, a serious blight of peach branches and leaves has appeared in the production of honey peaches in Fengqiao Town, Jiaxing. F...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12R1/645
Inventor 方丽王汉荣任海英
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES