Sodium hyaluronate and KGM porous bracket material and method for preparing same

A technology of konjac glucomannan and sodium hyaluronate, applied in the field of biomedical materials, can solve problems such as high cost and unsatisfactory performance of scaffold materials, and achieve the effects of uniform distribution, favorable wound healing, and good growth and proliferation state.

Active Publication Date: 2012-03-21
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a porous scaffold material of sodium hyaluronate and konjac glucomannan two biological materials and its preparation method, so as to solve the problems of unsatisfactory performance and high cost of existing scaffold materials

Method used

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  • Sodium hyaluronate and KGM porous bracket material and method for preparing same
  • Sodium hyaluronate and KGM porous bracket material and method for preparing same

Examples

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Embodiment 1

[0024] Embodiment 1: It is composed of sodium hyaluronate and konjac glucomannan, and the mass ratio of sodium hyaluronate to konjac glucomannan is 1:100.

[0025] see figure 1 , the preparation method of the sodium hyaluronate and konjac glucomannan porous scaffold material is:

[0026] (1) According to the mass ratio of sodium hyaluronate and water of 1:2000, dissolve sodium hyaluronate in water, stir it to fully dissolve, and then add alkaline solution into the solution; the concentration of alkaline solution is 0.1vol %%NH 3 ·H 2 O solution, the volume ratio of alkaline solution to water is 1:267.

[0027] (2) Cross-linking: according to the mass ratio of sodium hyaluronate to konjac glucomannan of 1:100, konjac glucomannan is added to the product obtained in step (1), and stirred until it is in a gel state;

[0028] (3) Seal the material obtained in step (2) and mature it in a water bath at a temperature of 70°C for 24 hours, take out the material, and then lower the ...

Embodiment 2

[0029] Embodiment 2: It is composed of sodium hyaluronate and konjac glucomannan, and the mass ratio of sodium hyaluronate to konjac glucomannan is 1:80.

[0030] see figure 1 , the preparation method of the sodium hyaluronate and konjac glucomannan porous scaffold material is:

[0031](1) According to the mass ratio of sodium hyaluronate and water of 1:100, dissolve sodium hyaluronate in water, stir it to fully dissolve, and then add alkaline solution into the solution; the concentration of alkaline solution is 0.8vol %% NaOH solution, the volume ratio of alkaline solution to water is 1:5000.

[0032] (2) Cross-linking: according to the mass ratio of sodium hyaluronate to konjac glucomannan of 1:80, konjac glucomannan is added to the product obtained in step (1), and stirred until it is in a gel state;

[0033] (3) Seal the product obtained in step (2) and mature it in a water bath at a temperature of 50°C for 12 hours to take out the material, then lower the temperature of...

Embodiment 3

[0034] Embodiment 3: It is composed of sodium hyaluronate and konjac glucomannan, the mass ratio of sodium hyaluronate and konjac glucomannan is 1:5.

[0035] see figure 1 , the preparation method of the sodium hyaluronate and konjac glucomannan porous scaffold material is:

[0036] (1) According to the mass ratio of sodium hyaluronate and water of 1:20, dissolve sodium hyaluronate in water, stir to make it fully dissolved, and then add alkaline solution into the solution; the concentration of alkaline solution is 1vol% % NH 3 ·H 2 O solution, the volume ratio of alkaline solution to water is 1:100.

[0037] (2) Cross-linking: according to the mass ratio of sodium hyaluronate to konjac glucomannan of 1:5, konjac glucomannan was added to the product obtained in step (1), and stirred until it was in a gel state;

[0038] (3) Seal the product obtained in step (2) and mature it in a water bath at a temperature of 90°C for 48 hours, take out the material, and then lower the tem...

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Abstract

The invention relates to a method for preparing a sodium hyaluronate and KGM (Konjac glucomannan) porous bracket material used in cartilage defect repair, belonging to the field of biomedicine materials. The invention adopts two materials, namely sodium hyaluronate and KGM. Through utilizing different proportions and the crosslinking of KGM, the sodium hyaluronate and KGM porous bracket material is prepared through adopting a freeze-drying method. The invention has mild reaction conditions, simple steps, and low cost; human body bone mesenchymal stem cells are uniformly distributed in the bracket material and can grow and reproduce well; and the aperture, the pore rate, the compressive strength, the hygroscopic coefficient, the degradation rate, and the like of the bracket material can be regulated through regulating the contents of the sodium hyaluronate, the KGM, and a cross-linking agent, as well as the freeze drying conditions, so as to get the porous bracket material meeting requirements. The material can be used in the cartilage repair field.

Description

technical field [0001] The invention relates to a sodium hyaluronate and konjac glucomannan porous scaffold material for cartilage defect repair and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] Articular cartilage mainly includes proteoglycan, collagen, and other proteins such as connexin, hyaluronic acid, etc., and its regeneration and self-repair ability is poor. At present, the commonly used repairing methods of articular cartilage injury include microfracture, subchondral drilling, autologous osteochondral transplantation and allogeneic osteochondral transplantation. Autologous osteochondral transplantation (mosaicplasty), safe and effective, is the most commonly used clinical treatment method, but this method will bring new local lesions to patients and its source is limited, so it cannot be used to repair cartilage defects with a diameter of more than 2cm , which greatly limits its application. Osteochond...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/20A61L27/56C08J9/28
Inventor 陈庆华董会王静陈佳黄明华
Owner KUNMING UNIV OF SCI & TECH
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