Method for producing feeding peanut peptide by anaerobic fermentation of peanut meal
An anaerobic fermentation, peanut peptide technology, applied in the application, animal feed, animal feed and other directions, can solve the problem of lower quality of feed raw materials, and achieve the effect of good application prospects
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Embodiment 1
[0018] 1. Preparation of Bacteria Liquid
[0019] 1. Streak culture of Lactobacillus fermentum strain on MRS medium plate for use.
[0020] Prepare 4.8 liters of MRS liquid medium (10.0 g casein peptone, 10.0 g beef extract, 5.0 g yeast extract, 5.0 g glucose, 5.0 g sodium acetate, 2.0 g diamine citrate, and Tween) in a 5-liter fermentation tank. 801.0 grams, 2.0 grams of dipotassium hydrogen phosphate, 0.2 grams of magnesium sulfate heptahydrate, 0.05 grams of manganese sulfate heptahydrate, 20.0 grams of calcium carbonate, 1.0 liter of distilled water), sterilized at 121°C, and sterilized at a rate of 3% after cooling. Lactobacillus fermentum undergoes anaerobic fermentation at 35°C with stirring every 3 hours. After 48 hours, the liquid bacterial solution was prepared.
[0021] 2. Streak the saccharomyces cerevisiae on the wort medium for use.
[0022] Prepare 3 liters of wort liquid medium in a 5 liter fermentor, sterilize at 121°C, and then aseptically plant Saccharomyces cerev...
Embodiment 2
[0030] 1. Preparation of Bacteria Liquid
[0031] 1. Streak culture of Lactobacillus fermentum strain on MRS medium plate for use.
[0032] Prepare 4.8 liters of MRS liquid medium (10.0 g casein peptone, 10.0 g beef extract, 5.0 g yeast extract, 5.0 g glucose, 5.0 g sodium acetate, 2.0 g diamine citrate, and Tween) in a 5-liter fermentation tank. 801.0 grams, 2.0 grams of dipotassium hydrogen phosphate, 0.2 grams of magnesium sulfate heptahydrate, 0.05 grams of manganese sulfate heptahydrate, 20.0 grams of calcium carbonate, 1.0 liters of distilled water), sterilized at 126°C, and sterilized at a rate of 4% after cooling. Lactobacillus fermentum undergoes anaerobic fermentation at 35°C with stirring every 3 hours. After 54 hours, the liquid bacterial solution was prepared.
[0033] 2. Streak the saccharomyces cerevisiae on the wort medium for use.
[0034] Prepare 3 liters of wort liquid medium in a 5 liter fermenter, sterilize at 126°C, and then aseptically plant Saccharomyces cere...
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