Method for producing feeding peanut peptide by anaerobic fermentation of peanut meal

An anaerobic fermentation, peanut peptide technology, applied in the application, animal feed, animal feed and other directions, can solve the problem of lower quality of feed raw materials, and achieve the effect of good application prospects

Inactive Publication Date: 2012-03-28
INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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AI-Extracted Technical Summary

Problems solved by technology

[0003] Since peanut meal is prone to produce aflatoxin during...
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Abstract

The invention relates to a method for producing feeding peanut peptides by anaerobic fermentation of peanut meal, which comprises the following steps: respectively preparing bacterial liquid of lactobacillus fermentum, saccharomyces cerevisiae, and bacillus subtillis, mixing peanut meal, corn meal, and potassium dihydrogen phosphate according to a mass ratio of 90:9:0.9:0.1 to prepare solid materials, well mixing the solid materials and mixed bacterial liquid according to a mass ratio of 6:4, performing fermentation, maintaining the fermentation temperature at 37 +/- 2 DEG C for 72-80 hours, when the fermentation is completed, drying the fermented materials till the water content is 11-12%, crushing to 60-70 meshes to obtain the feeding peanut peptides. The invention degrades the protein in the peanut meal into small peptides or amino acids to a great extent, and biodegrades the aflatoxin in the peanut meal; a lot of protease is generated during the fermentation process, and a large amount of lactic acids and a lot of beneficial microorganisms are contained in the product; the fermentation product is a good raw material for feed, and has good application prospects.

Application Domain

Animal feeding stuff

Technology Topic

Water contentChemistry +19

Examples

  • Experimental program(2)

Example Embodiment

[0017] Example 1
[0018] 1. Preparation of Bacteria Liquid
[0019] 1. Streak culture of Lactobacillus fermentum strain on MRS medium plate for use.
[0020] Prepare 4.8 liters of MRS liquid medium (10.0 g casein peptone, 10.0 g beef extract, 5.0 g yeast extract, 5.0 g glucose, 5.0 g sodium acetate, 2.0 g diamine citrate, and Tween) in a 5-liter fermentation tank. 801.0 grams, 2.0 grams of dipotassium hydrogen phosphate, 0.2 grams of magnesium sulfate heptahydrate, 0.05 grams of manganese sulfate heptahydrate, 20.0 grams of calcium carbonate, 1.0 liter of distilled water), sterilized at 121°C, and sterilized at a rate of 3% after cooling. Lactobacillus fermentum undergoes anaerobic fermentation at 35°C with stirring every 3 hours. After 48 hours, the liquid bacterial solution was prepared.
[0021] 2. Streak the saccharomyces cerevisiae on the wort medium for use.
[0022] Prepare 3 liters of wort liquid medium in a 5 liter fermentor, sterilize at 121°C, and then aseptically plant Saccharomyces cerevisiae at a rate of 3% after cooling. Perform aerobic fermentation at 35°C and stir every 3 hours. Fermentation is completed in 20 hours.
[0023] 3. Streak culture of Bacillus subtilis on the LB medium plate for use.
[0024] Prepare 3 liters of LB liquid medium in a 5 liter fermentor, sterilize at 121°C, and then aseptically plant Bacillus subtilis at a rate of 3% after cooling. Perform anaerobic fermentation at 35°C, stirring every 3 hours. After 20 hours, the liquid bacterial solution was prepared.
[0025] 4. Mix the three bacterial liquids with the same volume for use.
[0026] 2. Solid fermentation
[0027] The peanut meal, corn flour, yeast powder and potassium dihydrogen phosphate are mixed in a ratio of 90:9:0.9:0.1 to make a solid material (at this time, the total water content of the material is 11.5%). On the solid-liquid mixer, the solid material and the bacterial liquid are mixed uniformly at a mass ratio of 6:4, and the solid-liquid mixture is filled in a sealed container (1 cubic) with positive air cutting for fermentation, and the fermentation temperature is maintained at 35°C. After 72 hours, fermentation is complete.
[0028] The fermented material is dried to a moisture content of 12% and crushed to 60 meshes to obtain peanut peptides for feeding.

Example Embodiment

[0029] Example 2
[0030] 1. Preparation of Bacteria Liquid
[0031] 1. Streak culture of Lactobacillus fermentum strain on MRS medium plate for use.
[0032] Prepare 4.8 liters of MRS liquid medium (10.0 g casein peptone, 10.0 g beef extract, 5.0 g yeast extract, 5.0 g glucose, 5.0 g sodium acetate, 2.0 g diamine citrate, and Tween) in a 5-liter fermentation tank. 801.0 grams, 2.0 grams of dipotassium hydrogen phosphate, 0.2 grams of magnesium sulfate heptahydrate, 0.05 grams of manganese sulfate heptahydrate, 20.0 grams of calcium carbonate, 1.0 liters of distilled water), sterilized at 126°C, and sterilized at a rate of 4% after cooling. Lactobacillus fermentum undergoes anaerobic fermentation at 35°C with stirring every 3 hours. After 54 hours, the liquid bacterial solution was prepared.
[0033] 2. Streak the saccharomyces cerevisiae on the wort medium for use.
[0034] Prepare 3 liters of wort liquid medium in a 5 liter fermenter, sterilize at 126°C, and then aseptically plant Saccharomyces cerevisiae at a rate of 4% after cooling, and conduct aerobic fermentation at 35°C with stirring every 3 hours. Fermentation is completed in 24 hours.
[0035] 3. Streak culture of Bacillus subtilis on the LB medium plate for use.
[0036] Prepare 3 liters of LB liquid medium in a 5 liter fermentor, sterilize at 126°C, and then aseptically plant Bacillus subtilis at a rate of 4% after cooling. Perform anaerobic fermentation at 39°C with stirring every 3 hours. After 24 hours, the liquid bacterial solution was prepared.
[0037] 4. Mix the three bacterial liquids with the same volume for use.
[0038] 2. Solid fermentation
[0039] The peanut meal, corn flour, yeast powder and potassium dihydrogen phosphate are mixed in a ratio of 90:9:0.9:0.1 to make a solid material (at this time, the total water content of the material is 11.5%). On the solid-liquid mixer, the solid material and the bacterial liquid are mixed uniformly at a mass ratio of 7:3, and the solid-liquid mixture is filled in a sealed container (1 cubic) with positive air cutting for fermentation, and the fermentation temperature is maintained at 37°C. After 80 hours, fermentation is complete.
[0040] The fermented material is dried to a moisture content of 11% and crushed to 70 meshes to obtain peanut peptides for feeding.
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  • Good application prospect
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