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Method for degumming crude soybean oil by immobilized phosphatidase membrane

A technology of immobilized phospholipase and immobilized enzyme, which is applied in the direction of fat oil/fat refining and fat production, can solve the problems of difficult temperature and pH control, high cost, poor degumming effect of soybean crude oil, etc., and achieve high retention activity, Good vitality, high stability effect

Inactive Publication Date: 2012-04-04
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problem that the existing enzyme degumming method has temperature and pH that are not easy to control, which leads to the poor degumming effect of soybean crude oil and the high cost of the existing enzyme degumming method, and proposes to use an immobilized phospholipase membrane for The method of soybean oil degumming

Method used

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specific Embodiment approach 1

[0006] Specific embodiment one: In this embodiment, the immobilized phospholipase membrane is used for the degumming of crude soybean oil through the following steps: 1. Preparation of composite membrane: 15g cellulose acetate is dissolved in 85g dimethylformamide at 60°C to obtain Casting solution. The polytetrafluoroethylene membrane was first cleaned with 0.1mol / L NaOH solution and 0.1mol / L HCl solution, then cleaned with deionized water, dried in vacuum, and spread on a clean glass plate. To control the thickness, apply the cellulose acetate casting solution on the surface of the polytetrafluoroethylene film with a special scraper, volatilize at room temperature for an appropriate time to complete the dry phase transition, and then immerse in deionized water to complete the wet phase transition. After 12 hours, wash with deionized water and dry naturally for use; 2. Immobilization of phospholipase: take several parts of 1 cm 2 Add 0.06 g / mL enzyme solution and disodium hy...

specific Embodiment approach 2

[0007] Specific embodiment two: The difference between this embodiment and specific embodiment one is that in step three, add a citric acid solution with a mass concentration of 40-45% to the treated soybean oil and stir at a speed of 600-1600 rpm 8 to 18 minutes. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0008] Specific embodiment three: The difference between this embodiment and specific embodiment one is that in step three, adding a citric acid solution with a mass concentration of 42 to 44% in the processed soybean oil is carried out at a speed of 800 to 1200 rpm Stir for 10-14 minutes. Other steps are the same as in the first embodiment.

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Abstract

The invention relates to a method for degumming crude soybean oil by an immobilized phosphatidase membrane. The method allows problems that the soybean oil degumming effect is bad because the temperature and the pH are difficult to control in present enzyme degumming methods and the cost of the present enzyme degumming methods is high to be solved. The immobilization of an enzyme is in favor of the dispersion, the recovery and the reutilization of the enzyme, so the loss of the enzyme and the repollution of the residual enzyme are reduced. The method for degumming the crude soybean oil by the immobilized phosphatidase membrane comprises the following steps: 1, preparing a composite membrane; 2, immobilizing phosphatidase; 3, carrying out early stage processing on soybean oil; 4, adding the immobilized phosphatidase to the soybean oil; and 5, stirring, and centrifuging. So the crude soybean oil is degummed by the immobilized phosphatidase membrane. By adopting the method in the invention to degum the crude soybean oil, the degumming efficiency is 20-30% higher than the degumming efficiency of the present degumming methods, and the production cost is 20-40% lower than the production cost of the present degumming methods.

Description

Technical field: [0001] The invention relates to a method for degumming soybean crude oil by immobilizing phospholipase film. Background technique: [0002] Phospholipase A 1 It is a carboxylate hydrolase that specifically hydrolyzes Sn-1 fatty acid in phospholipids to generate lysophospholipids and fatty acids. It is widely used in phospholipid modification and oil refining. free phospholipase A 1 Expensive and not easy to recycle. The immobilized phospholipase is beneficial to the dispersion, recovery and reuse of the enzyme, and reduces the re-pollution caused by the residual enzyme. The immobilization of phospholipase with cellulose acetate / polytetrafluoroethylene composite membrane has the advantages of good chemical stability and excellent mechanical properties, which improves the activity and stability of the enzyme in the reaction system, which is beneficial to the recovery of the enzyme and the product production. [0003] Soybean oil contains about 3% phosphol...

Claims

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Application Information

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IPC IPC(8): C11B3/00C11B3/04C11B3/06
Inventor 于殿宇王立琦韩建春程建军周晓丹张佳宁时敏陈晓慧李志平王雪王妍李越周旋奚会松宋云花
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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