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Manufacturing method of tobacco leaf primary color herbarium specimen

A technology of wax leaf specimens and production methods, which is applied to the preparation of test samples, etc., can solve the problems of prolonged pressing time, high technical difficulty, expensive equipment, etc., and achieve the effects of maintaining integrity, improving economy, and high scientific research value

Active Publication Date: 2013-04-17
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has been proved by practice that the above method is not suitable for making natural color wax leaf specimens of tobacco leaf spot disease: rapid dehydration method with electric iron, rapid dehydration method in microwave oven, low temperature dehydration drying method, silica gel fine powder water absorption method will easily cause lesion perforation, fall off, leaves The color turns yellow and distorted, the leaves are dry and brittle, and the veins are damp and moldy; although the copper acetate liquid boiling method and the boiling water rapid boiling method can maintain the original color of the leaves, it is also easy to cause the lesions to perforate and fall off. Pressing and drying are required, the steps are complicated, the pressing time is prolonged, and there are many uncontrollable factors in the process, resulting in poor repeatability
The Chinese invention patent "Method for making snow lotus specimens" (ZL 02108951.5) discloses a method of preparing snow lotus specimens with original colors and original colors by freezing and vacuuming. Drying and vacuuming methods to make specimens are relatively expensive equipment, cumbersome operations, and technical difficulties, so its application range is greatly limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take the specimen making of leaves with necrotic leaf spot of tobacco leaves caused by PVY virus as an example.

[0031] A. Choose a specimen holder that is 1.0cm longer and wider than the specimen, place the specimen holder flat, and place 3 layers of bedding paper, 3 layers of absorbent paper, and a layer of granular silica gel desiccant with a thickness of 0.5cm on it. 3 layers of absorbent paper, and then lay the first specimen and shape it; then lay 3 to 5 layers of absorbent paper, a layer of revived granular silica gel desiccant with a thickness of 1.0cm, and 3 layers of absorbent paper, then place the second specimen, In this way, stack the third sample until the fifth sample, and then lay 3 layers of absorbent paper, a layer of resurrected granular silica gel desiccant with a thickness of 0.5cm, 3 layers of absorbent paper, and 3 layers of bedding paper. Finally, close the specimen holder and tie it up as a whole;

[0032] B. Place the tightly bound specimen h...

Embodiment 2

[0037] Take the specimen of tobacco red spot diseased leaf caused by Alternaria pathogenic fungus as an example.

[0038]A. Select a specimen holder that is 1.5cm longer and wider than the specimen, place the specimen holder flat, and place 5 layers of bedding paper, 5 layers of absorbent paper, and a granular activated carbon desiccant layer with a thickness of 0.8cm on it. 5 layers of absorbent paper, and then lay the first specimen and shape it; then lay 5 layers of absorbent paper, a layer of reactivated granular activated carbon desiccant with a thickness of 1.6cm, and place the second specimen after 5 layers of absorbent paper, and so on Then stack the third sample until the eighth sample, and then lay 5 layers of absorbent paper, a layer of activated carbon desiccant with a thickness of 0.8cm, 5 layers of absorbent paper, and 5 layers of bedding paper, and finally put them together. Put on the specimen holder and tie it up as a whole;

[0039] B. Place the tightly boun...

Embodiment 3

[0044] Take the tobacco wildfire disease leaf specimens produced by Pseudomonas syringae pv. tobacco pathogenic bacteria as an example.

[0045] A. Choose a specimen holder that is 2.0cm longer and wider than the specimen, place the specimen holder flat, and lay 4 layers of bedding paper, 4 layers of absorbent paper, and 0.7cm thick granular attapulgite minerals in order to dry layer and 4 layers of absorbent paper, and then lay the first specimen and shape it; then lay 4 layers of absorbent paper, a revived granular attapulgite mineral desiccant layer with a thickness of 1.3cm, and 4 layers of absorbent paper, and then place the second specimen 2 specimens, stack the 3rd specimen in this way until the 6th specimen, and then lay 4 layers of absorbent paper, 0.7cm-thick revived granular attapulgite mineral desiccant layer, 4 layers of absorbent paper paper, 4 layers of bedding paper, and finally close the specimen holder and tie it tightly;

[0046] B. Place the tightly bound ...

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Abstract

The invention provides a manufacturing method of a tobacco leaf primary color exsiccate. The method comprises the steps of shaping, color fixing, drying and moisturizing again. The shaping step mainly comprises: orderly overlapping absorbing paper, a moisture absorbent, absorbing paper and a specimen in a specimen holder; orderly overlapping the specimens in n layers (preferably 5-8 layers) according to the above form; fixing color for 15-18 hours at the constant temperature of 45-55 DEG C; drying for 3-6 hours at the constant temperature of 55-65 DEG C; and placing the absorbing paper and specimen in a room temperature environment with relative moisture of more than 70%, moisturizing again for 6-12 hours to obtain the primary color exsiccate. The specimen manufacturing is faster by usingthe method provided by the invention, and 6-8 days are shortened in comparison with the general pressing method, the color retention effect is good, and the color keeps the same in the specimen retention period; the water content is controlled in a range of 8-10% so as to prevent the specimen from brittle rupture and maintain the integrity of the specimen. The manufacturing method provided by theinvention is especially suitable for the manufacturing of the tobacco leaf primary color exsiccate, is especially in favor of storing the primary shape and color of the diseased tobacco leaf, and greatly improves the scientific research value and economy of the specimen.

Description

technical field [0001] The invention belongs to the technical field of making plant specimens, and in particular relates to a method for preparing cured leaf specimens with simple and quick operation and capable of maintaining the original color of tobacco leaves, and is especially suitable for making preserved leaf specimens with original color of tobacco leaf spot disease. Background technique [0002] In the prior art, the preparation methods of the primary color wax leaf specimens of plant diseases include copper acetate liquid boiling method, electric iron rapid dehydration method, microwave oven rapid dehydration method, low temperature dehydration drying method, boiling water rapid boiling method (see " wax leaf specimen preservation Green production technology", "Henan Forestry", Liu Jianhua 2000 (6): 24), and silica gel fine powder water absorption method ("Introduction of a production method to maintain the original color of wax leaf specimens", "Biology Bulletin", ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28
Inventor 方敦煌莫笑晗晋艳宋春满秦西云余清
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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