Application of three compounds extracted from starfish to promotion of bone fracture healing, and preparation method for three compounds

A compound and starfish technology, applied in the field of promoting fracture healing and compound preparation, can solve the problem of no starfish, and achieve the effects of low cost, quick effect and sufficient drug source.

Active Publication Date: 2012-04-18
大连美罗中药厂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant report on the application of starfish in the treatment of bruises

Method used

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  • Application of three compounds extracted from starfish to promotion of bone fracture healing, and preparation method for three compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Take 3.0 kg of medicinal materials of Roche haipanche, steam them with circulating steam for 2-4 hours, then put them in an oven, dry them at 60°C-80°C, take them out, let them cool, crush them, and pass them through an 80-mesh sieve. Use 3 times the amount of 85% ethanol to extract, heat and reflux for 3 times, each time is 30 minutes, the extracts are combined, concentrated under reduced pressure, and dried to obtain 500 grams of total extract.

[0016] 2. The total extract is dispersed with water, followed by petroleum ether (30-60 0 C), ethyl acetate and n-butanol were extracted three times in equal volume to obtain 85 g of n-butanol layer and 18 g of ethyl acetate layer.

[0017] 3. The ethyl acetate layer was separated by a silica gel column, 240 grams of 100-200 mesh silica gel, using chloroform-acetone (20:1) as the eluent, and the obtained fraction was further used for preparative thin-layer chromatography, and petroleum ether-acetone The mobile phase was 5...

Embodiment 2

[0020] 1. Take 5.0 kg of sea spinach medicinal material, steam it with circulating steam for 2-4 hours, then put it in an oven, dry it at 60°C-80°C, take it out, let it cool, crush it, and pass it through a 80-mesh sieve. Use twice the amount of 70% ethanol to extract, heat and reflux twice, each time is 60 minutes, the extracts are combined, concentrated under reduced pressure, and dried to obtain 800 grams of total extract.

[0021] 2. The total extract is dispersed with water, followed by petroleum ether (30-60 0 C), ethyl acetate and n-butanol were extracted four times in equal volumes to obtain 130 g of n-butanol layer and 26 g of ethyl acetate layer.

[0022] 3. The ethyl acetate layer was separated by a silica gel column, 300 grams of 100-200 mesh silica gel, using chloroform-acetone (20:1) as the eluent, and the obtained fraction was further used for preparative thin-layer chromatography, and petroleum ether-acetone 5:1 as the mobile phase, purified to obtain 23 mg of...

Embodiment 3

[0025] 1. Take 2.0 kg of Dow’s sun starfish medicinal material, steam it with circulating steam for 2-4 hours, then put it in an oven, dry it at 60°C-80°C, take it out, let it cool, crush it, and pass it through a 80-mesh sieve. Use methanol to extract and heat and reflux for 5 times, each time is 40 minutes. The extracts are combined, concentrated under reduced pressure, and dried to obtain 400 grams of total extract.

[0026] 2. The total extract is dispersed with water, followed by petroleum ether (30-60 0 C), ethyl acetate and n-butanol were extracted four times in equal volumes to obtain 70 g of n-butanol layer and 10 g of ethyl acetate layer.

[0027] 3. The ethyl acetate layer was separated by a silica gel column, 200 grams of 100-200 mesh silica gel, using chloroform-acetone (20:1) as the eluent, and the obtained fraction was further used for preparative thin-layer chromatography, and petroleum ether-acetone 5:1 as the mobile phase, purified to obtain 5 mg of compound...

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Abstract

The invention discloses application of three compounds extracted from starfish to the promotion of bone fracture healing, and a preparation method for the three compounds, and belongs to the field of methods for extracting Chinese medicines. The preparation method for the three compounds comprises the following steps of: crushing a starfish medicinal material, extracting by using a solvent, mixing extracting solutions, concentrating under reduced pressure, and drying to obtain a total extract; performing equal-volume extraction on the total extract by using ethyl acetate and n-butanol to obtain an n-butanol layer and an ethyl acetate layer; separating the ethyl acetate layer by a silicagel column, and purifying to obtain the compound 3, namely ergosta-7,22-diene-3beta,5,6-triol and the compound 1, namely cyclo(leucine-proline)dipeptide; and separating the n-butanol layer by the silicagel column to obtain the compound 2, namely cyclo(serine-leucine)dipeptide. The compounds have a good effect of promoting bone fracture healing, so that the application prospect of the three compounds used as novel medicines for promoting bone fracture healing is showed.

Description

technical field [0001] The invention belongs to the field of extraction methods of traditional Chinese medicines, in particular to a preparation method of a starfish compound and its application in promoting fracture healing. Background technique [0002] In recent years, the research on the active ingredients and pharmacological activity of starfish has attracted more and more attention at home and abroad. So far, researchers from various countries have successively obtained more than a dozen types of chemical substances from starfish, including saponins, sterols, alkaloids, anthraquinones, polypeptides, amino acids, polysaccharides, lipids, proteins and other chemical components. Studies have shown that starfish extract and its compound preparations have various biological and physiological functions such as anti-cancer, anti-arrhythmia, lowering blood pressure, promoting learning and memory, antibacterial and anti-virus, hemolysis, and anti-fatigue. It is used to treat a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D487/04C07D241/08C07J9/00A61K31/4985A61K31/495A61K31/575A61P19/08
Inventor 张成海周文波李冬梅
Owner 大连美罗中药厂有限公司
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