Comprehensive extraction method for platycodin and platycodon grandiflorum polysaccharide

A technology for comprehensive extraction and platycodon polysaccharides, which is applied in the direction of drug combinations, pharmaceutical formulas, and medical preparations containing active ingredients, to achieve high extraction rates, improve efficiency, and reduce environmental pollution

Inactive Publication Date: 2012-04-25
HUAINAN UNITED UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the current single method for extracting platycodon saponin or platycodon polysaccharide, and provide a method for comprehensively extracting platycodon saponin or platycodon polysaccharide with low cost, comprehensive utilization of raw materials, and little environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for comprehensively extracting platycodon saponins and platycodon polysaccharides, comprising the steps of:

[0025] (1) Add water with a mass multiple of 10 times milliliters to the fresh Platycodon grandiflorum, make a slurry, process it in a 200W ultrasonic device for 20min, and then filter;

[0026] (3) The filtered supernatant is centrifuged in a centrifuge (5000r / min, 30min) to further remove the precipitate;

[0027] (3) The supernatant after centrifugation is ultrafiltered by an ultrafiltration device with a molecular weight cut-off of 5000 to obtain a permeate A and a retentate B;

[0028] (4) The macroporous resin HPD100 on the permeate liquid A was eluted with 70% ethanol to obtain an eluent, which was concentrated under reduced pressure and then dried to obtain platycodon saponin powder;

[0029] (5) The retentate B was concentrated under reduced pressure, then added ethanol to adjust the ethanol concentration to 75%, stood still for 8 hours, colle...

Embodiment 2

[0032] A method for comprehensively extracting platycodon saponins and platycodon polysaccharides, comprising the steps of:

[0033] (1) Add 10 times the mass of water in milliliters to fresh Platycodon grandiflorum, make a slurry, process in a 100W ultrasonic device for 30min, and then filter;

[0034] (2) The filtered supernatant is centrifuged in a centrifuge (5000r / min, 30min) to further remove the precipitate;

[0035] (3) The supernatant after centrifugation is ultrafiltered by an ultrafiltration device with a molecular weight cut-off of 5000 to obtain a permeate A and a retentate B;

[0036] (4) Elute the macroporous resin D101 on the permeate A with 70% ethanol to obtain an eluent, concentrate the eluent under reduced pressure, and then dry to obtain platycodon saponin powder;

[0037] (5) The retentate B was concentrated under reduced pressure, then added ethanol to adjust the ethanol concentration to 75%, stood still for 8 hours, collected the precipitate, redissolv...

Embodiment 3

[0040] A method for comprehensively extracting platycodon saponins and platycodon polysaccharides, comprising the steps of:

[0041] (1) Add 10 times the mass of water in milliliters to fresh Platycodon grandiflorum, make a slurry, process it in a 300W ultrasonic device for 10 minutes, and then filter;

[0042] (2) The filtered supernatant is centrifuged in a centrifuge (5000r / min, 30min) to further remove the precipitate;

[0043] (3) The supernatant after centrifugation is ultrafiltered by an ultrafiltration device with a molecular weight cut-off of 5000 to obtain a permeate A and a retentate B;

[0044] (4) Elute the macroporous resin AB-8 on the permeate A with 70% ethanol to obtain an eluent, concentrate the eluent under reduced pressure, and then dry to obtain platycodon saponin powder;

[0045] (5) The retentate B was concentrated under reduced pressure, then added ethanol to adjust the ethanol concentration to 75%, stood still for 8 hours, collected the precipitate, red...

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PUM

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Abstract

The present invention relates to a comprehensive extraction method for platycodin and platycodon grandiflorum polysaccharide. According to the method, platycodon grandiflorum is sequentially subjected to treatments of beating, ultrasonic extraction, filtration, and centrifugation; the resulting clarified solution is subjected to ultrafiltration; the resulting supernatant is subjected to ultrafiltration by a ultrafiltration device to obtain a permeating solution and a retention solution; the permeating solution is loaded on macroporous resin, and an elution treatment is performed by ethanol; the resulting eluent is subjected to vacuum concentration and drying to obtain the platycodin powder; the retention solution is subjected to vacuum concentration, then ethanol is added to the resulting concentrated solution; treatments of standing, precipitation collecting, water reconstituting, freeze drying are performed to obtain the platycodon grandiflorum polysaccharide powder. With the present invention, the yield of the method is high, the comprehensive utilization of the raw materials is achieved, and the cost is low.

Description

technical field [0001] The invention relates to an extraction method, in particular to a comprehensive extraction method for simultaneously extracting platycodon saponin and platycodon polysaccharide from fresh platycodon. Background technique [0002] Platycodon grandiflorum (Jacq.) A.DC. is usually the root of Platycodon grandiflorum, a plant of the genus Platycodon in the family Campanulaceae. Some studies have been done on the medicinal value of Bellflower at home and abroad. Preliminary studies have shown that Bellflower has immune regulation, anti-inflammatory, expectorant, and liver protection. Platycodon saponins and platycodon polysaccharides are the two main active ingredients of platycodon. [0003] At present, the extraction methods of platycodon saponin mainly include alcohol soaking method, reflux heat extraction method, ultrasonic extraction method and supercritical extraction method. [0004] Wu Jingtao et al. (Wu Jingtao, Yang Guiwen. Preparation of Platyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/34C08B37/00A61P37/02A61P29/00A61P11/10A61P1/16A61K125/00A61K127/00A61K135/00
Inventor 靳学远秦霞何晓文刘红郭红彦
Owner HUAINAN UNITED UNIVERSITY
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