Genotyping chip for legionella pneumophila, and kit for detection of legionella pneumophila

Abstract

The present invention provides a genotyping chip for legionella pneumophila, and a kit for detection of the legionella pneumophila. The chip and the kit are mainly provided according to the 11 serotypes of the legionella pneumophila, wherein the 11 serotypes comprise O1, O2, O3, O4, O5, O6, O7, O11, O12, O13 and O15. The gene chip comprises a solid phase carrier and oligonucleotide probes fixed on the solid phase carrier, wherein the oligonucleotide probes comprise DNA fragments selected from wzm gene, wzt gene and wecA gene, or complementary DNA fragments of the wzm gene, the wzt gene and the wecA gene, wherein the wzm gene, the wzt gene and the wecA gene have significant biological evolution advantages. According to the present invention, the genomic DNA of the sample requiring detection is amplified by the designed primers; the resulting amplified genomic DNA is labeled; the resulting labeled genomic DNA is subjected to hybridization with the gene chip; according to the resulting hybridization signals, the different serotypes of the legionella pneumophila can be detected. With the gene chip of the present invention, the purpose of the detection of the serotypes of the legionella pneumophila can be achieved, the operation is convenient, the accuracy is high, the repeatability is strong, and the accurate medical medication guidance is provided.

Description

technical field

[0001] The invention relates to a gene chip and a detection kit containing the chip, in particular to Legionella pneumophila O1, O2, O3, O4, O5, O6, O7, O11, O12, O13 and O15, which are important pathogens in water bodies A total of 11 serotype gene chips and detection kits. Background technique

[0002] Water is a necessary condition for the existence of life. It enables us to multiply and thrive. Human life, production and entertainment cannot do without water. It is also a place and carrier for many pathogenic microorganisms to breed and spread. Once these pathogenic microorganisms enter the human body, they may cause illness or even death, seriously threatening human health. With the development of society and the improvement of living standards, people are more and more concerned about their own health issues, and the safety of various water bodies (including drinking water, rivers and lakes, swimming pools, etc.) has increasingly become a hot spot of c...

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