Method for recovering intraoperative autoblood

An autologous blood recovery and autologous blood technology, applied in the direction of blood circulation treatment, etc., can solve the problems of red blood cell number and function damage, the inability to recover the blood of tumor patients, and the lack of disinfection and removal of tumor cells in the blood recovery machine. and function, the effect of ensuring quality

Inactive Publication Date: 2012-05-02
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, intraoperative autologous blood salvage also has its contraindications. Because the blood salvage machine does not have the function of disinfection and removal of tumor cells, the blood of patie...

Method used

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  • Method for recovering intraoperative autoblood
  • Method for recovering intraoperative autoblood
  • Method for recovering intraoperative autoblood

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Experimental program
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Embodiment 1

[0026] An embodiment of the present invention, such as figure 1 As shown, the blood recovery machine was used to recover the autologous blood during the operation. The washing solution 5 was MAP red blood cell preservation solution, and the anticoagulant solution 3 was prepared by MAP red blood cell preservation solution and heparin sodium at a ratio of 1000ml: 300mg. The blood was filtered through the laminated 16-layer acetate non-woven filter membrane with a pore size of 11 μm and stored in the blood bag.

Embodiment 2

[0028] Another embodiment of the present invention, such as figure 1 As shown, a blood salvage machine is used to recover autologous blood during the operation. The washing solution 5 is MAP red blood cell preservation solution, and the anticoagulant solution 3 is prepared by MAP red blood cell preservation solution and heparin sodium at a ratio of 1000ml: 200mg. The blood was filtered through 7 layers of acetate non-woven filter membrane with a pore size of 20 μm and stored in the blood bag.

Embodiment 3

[0030] A kind of intraoperative autologous blood salvage method provided by the present invention, such as figure 1 As shown, the blood recovery machine was used to recover the autologous blood during the operation. The washing solution 5 was MAP red blood cell preservation solution, and the anticoagulant solution 3 was prepared by MAP red blood cell preservation solution and heparin sodium at a ratio of 1000ml: 400mg. The blood was filtered through 28 layers of acetate non-woven filter membrane with a pore size of 15 μm and stored in the blood bag.

[0031] The experimental data of technical effect of the present invention is provided below:

[0032] (1) Minimize the number of nucleated cells: By using the red blood cell washing solution in the technology of the present invention, the adhesion characteristics of nucleated cells are changed, and the adhesion ability to the filter membrane is further enhanced, so that the nucleated cells The ability to remove is greatly en...

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Abstract

The invention provides a method for recovering intraoperative autoblood. Intraoperative autoblood is recovered by using a blood recovering machine; an MAP (Mannitol-Adenine-Phosphate) erythrocyte preserving fluid is taken as a cleaning solution; an anti-freezing liquid is prepared from the MAP erythrocyte preserving fluid and sodium heparin in the ratio of 1,000ml:(200-400mg); and blood treated by using the blood recovering machine is stored in a blood bag after being filtered with an acetic ester non-woven fabric filter film which has the pore diameter of 11-20 mum and is formed by laminating 7-40 layers. Compared with the prior art, the method for recovering intranperative autoblood disclosed by the invention has the advantages that: karyocytes including tumor cells in blood are removed, the erythrocyte quantity and function are protected to the maximum extent, and the quality of the recovered blood is ensured.

Description

technical field [0001] The invention relates to a blood recovery method, in particular to an intraoperative autologous blood recovery method. Background technique [0002] Intraoperative autologous blood salvage is not a new term for many people. It mainly sucks autologous blood and a certain amount of heparin into the blood storage tank through negative pressure suction, and concentrates the red blood cells in the processing container through high-speed centrifugation (centrifugation) cup), while lighter material (plasma, clotting factors, rinsing fluid, free hemoglobin, cell debris) overflows from the processing container into a waste bag. When the processing container is full of red blood cells, inject physiological saline for washing. The cells are washed with normal saline, the white blood cells, platelets, and free hemoglobin are repeatedly diluted and discharged, the centrifugation is stopped, and the washed red blood cells are pumped into the blood transfusion bag t...

Claims

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Application Information

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IPC IPC(8): A61M1/38
Inventor 刘进杜磊丁五行
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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