Continuous preparation method of high-quality ethyl linolenate

A technology of ethyl linolenic acid and linolenic acid, which is applied in the field of supercritical continuous processing technology, can solve the problems of unclear antioxidant protection, product quality defects, and disconnection.

Inactive Publication Date: 2012-05-02
SHANGHAI ZHONGPEI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to solve the problems that the existing linseed oil extraction section and the linolenic acid separation and purification section are disconnected, the anti-oxidation protection is not clear in the process of linolenic acid separation and purification, and the quality of the final product is flawed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Put 200 grams of crushed perilla seeds into a supercritical extraction tank to adjust the CO 2 The pressure is 35MPa, the temperature is 40°C, extraction is carried out for 90 minutes, and the pressure is reduced to 6MPa, and the perilla seed oil is precipitated at 35°C. CO control 2 At a pressure of 16 MPa and 30° C., 162 grams of ethanol, 2.7 grams of lipase, and 1.8 milliliters of water were added and reacted for 2 hours. regulate CO 2 The pressure is 35MPa, and the temperature is 40°C for extraction. The pressure was reduced to 6MPa, and the ethyl linolenic acid mixture was precipitated at 35°C. Improve CO 2 The pressure is 15MPa, the temperature is 30°C, and the urea silica gel column adsorption is implemented. with entrained petroleum ether CO 2 Eluted, distributed and collected to obtain 34.6 g of ethyl linolenic acid with a purity of 88.1%.

Embodiment 2

[0012] Put 200 grams of crushed linseed into a supercritical extraction tank to adjust the CO 2 The pressure is 33MPa, the temperature is 45°C, the extraction is for 120 minutes, the pressure is reduced to 6MPa, and the linseed oil is precipitated at 35°C. CO control 2 At a pressure of 12 MPa and 40° C., 129 grams of ethanol, 2.2 grams of lipase, and 1.5 milliliters of water were added and reacted for 2 hours. regulate CO 2 The pressure is 35MPa, and the temperature is 40°C for extraction. The pressure was reduced to 6MPa, and the ethyl linolenic acid mixture was precipitated at 35°C. Improve CO 2 The pressure is 10MPa, the temperature is 35°C, and the urea silica gel column adsorption is implemented. with entrained petroleum ether CO 2 Eluted, distributed and collected to obtain 26.5 grams of ethyl linolenate with a purity of 86.4%

Embodiment 3

[0014] Put 200 grams of crushed Zanthoxylum bungeanum seeds into a supercritical extraction tank, and adjust the CO 2 The pressure is 38MPa, the temperature is 35°C, the extraction is for 60 minutes, the pressure is reduced to 6MPa, and the pepper seed oil is precipitated at 35°C. CO control 2 At a pressure of 14 MPa and 35° C., 33.6 grams of ethanol, 0.6 grams of lipase, and 0.4 milliliters of water were added and reacted for 2 hours. regulate CO 2 The pressure is 35MPa, and the temperature is 40°C for extraction. The pressure was reduced to 6MPa, and the ethyl linolenic acid mixture was precipitated at 35°C. Improve CO 2 The pressure is 12MPa, the temperature is 33°C, and the urea silica gel column adsorption is carried out. with entrained petroleum ether CO 2 Eluted, distributed and collected to obtain 7.2 grams of ethyl linolenate with a purity of 82.1%

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Abstract

The invention discloses a supercritical continuous processing process from the extraction of linolenic acid-containing oil to the hydrolysis and esterification of thelinolenic acid and the separation and purification of ethyl linolenate. The technical proposal of the continuous processing process comprises the following steps of: crushing a natural plant containing the linolenic acid oil, extracting the oil by means of the characteristics of CO2 supercritical extraction, hydrolyzing the oil using lipase in a supercritical environment so as to release fatty acid, and performing ethyl-esterification on the fatty acid by means of supercritical high pressure catalysis; next, separating out ethyl linolenate by means of an ethanol entrainer; and finally, performing urea column chromatography to obtain high-purity ethyl linolenate by means of the high solubility and low viscosity characteristics of the supercritical fluid. Moreover, contact with oxygen is avoided in the processing process, high-quality ethyl linolenate product can be obtained.

Description

technical field [0001] The invention relates to a preparation and purification process of α-ethyl linolenic acid, more specifically to a supercritical continuous processing process from extraction of oil containing linolenic acid to hydrolysis and esterification of linolenic acid and separation and purification of ethyl linolenic acid. Background technique [0002] α-linolenic acid is an all-cis 9,12,15-octadecatrienoic acid, which is an omega-3 series polyunsaturated fatty acid. α-linolenic acid generates a series of metabolites through dehydrogenation and carbon chain extension, among which EPA and DHA are important. Medical research has proved that α-linolenic acid has the functions of lowering blood fat, lowering cholesterol, promoting fat metabolism, liver cell regeneration, anti-cancer, etc., and its function on human health has been highly valued. The human body cannot produce α-linolenic acid and can only be ingested from exogenous foods. Therefore, the method of mo...

Claims

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Application Information

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IPC IPC(8): C12P7/64C07C69/587C07C67/56
CPCY02P20/54
Inventor 周家春陆琦芳
Owner SHANGHAI ZHONGPEI BIOLOGICAL TECH
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