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Method for preparing natural haematochrome zinc porphyrin

A technology of natural red pigment porphyrin zinc, applied in the field of preparation of natural red pigment porphyrin zinc, to achieve the effects of avoiding health hazards, good color rendering effect, and simple and easy preparation method

Active Publication Date: 2012-05-02
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies on the synthesis and preparation of porphyrin zinc at present. The present invention aims to prepare natural red pigment porphyrin zinc, which is healthy and safe, and has broad market prospects.

Method used

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  • Method for preparing natural haematochrome zinc porphyrin
  • Method for preparing natural haematochrome zinc porphyrin
  • Method for preparing natural haematochrome zinc porphyrin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Zn 2+ Preparation of chelatase

[0029] (a) Clean beef liver, pig liver, and animal heart, weigh 100 g of evenly chopped beef liver, pig liver, and animal heart, put them into a pre-cooled tissue grinder, add cooling distilled water of equal quality to grind it.

[0030] (b) Centrifuge the minced meat at 15,000 g for 8-15 min at 4°C, and remove the supernatant.

[0031] (c) The cell tissue in the lower layer was dissolved and mixed with distilled water, centrifuged under the same conditions, and the operation was repeated 2-3 times.

[0032] (d) The obtained cell tissue pellet was stored at 4°C for future use.

[0033] 2. BBD response surface experimental design optimization

[0034] 2.1 Experimental method

[0035] (a) Weigh the prepared Zn respectively 2+ Chelatease, hemin and zinc sulfate powder, add distilled water to dissolve to a total volume of 100mL, mix well;

[0036] (b) The mixed solution is placed in a water bath, and reacted at 60° C. for 6 hours; ...

Embodiment 2

[0060] (1)Zn 2+ Preparation of chelatase

[0061] With embodiment 1.

[0062] (2) Preparation of porphyrin zinc:

[0063] (a) Weigh the prepared Zn respectively 2+ Chelatease, protoporphyrin disodium and zinc chloride powder, add distilled water to a total volume of 100mL to dissolve and mix, so that Zn 2+ The concentration of chelatase is 0.1g / mL, the concentration of disodium protoporphyrin is 1g / L, and the concentration of zinc chloride is 0.1g / L;

[0064] (b) The mixed solution is placed in a water bath, and reacted at 55° C. for 7 hours;

[0065] (c) 75% acetone of 1 times the volume of the above mixed solution was added to extract zinc porphyrin, and the concentration of zinc porphyrin was detected by the fluorescence spectrophotometry described in Example 1 to be 985.34 mg / L.

[0066] (3) Separation and purification of porphyrin zinc:

[0067] The prepared porphyrin zinc is separated and purified by high performance liquid chromatography, which specifically includ...

Embodiment 3

[0075] (1)Zn 2+ Preparation of chelatase

[0076] With embodiment 1.

[0077] (2) Preparation of porphyrin zinc:

[0078] (a) Weigh the prepared Zn respectively 2+ Chelatease, hemin and zinc citrate powder, add distilled water to a total volume of 100mL to dissolve and mix, so that Zn 2+ The concentration of chelatase is 0.2g / mL, the concentration of hemin is 3g / L, and the concentration of zinc citrate is 0.8g / L;

[0079] (b) The mixed solution is placed in a water bath, and reacted at 60°C for 5.5h;

[0080] (c) Adding 75% acetone twice the volume of the above mixed solution to extract porphyrin zinc, the concentration of porphyrin zinc detected by the fluorescence spectrophotometry described in Example 1 was 673.21mg / L.

[0081] (3) Separation and purification of porphyrin zinc:

[0082] The prepared porphyrin zinc is separated and purified by high performance liquid chromatography, which specifically includes the following steps:

[0083] (a) Set the column temperatu...

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Abstract

The invention belongs to the field of foods and discloses a method for preparing natural haematochrome zinc porphyrin. The method comprises the following steps of: (1) preparing Zn<2+> chelatase by using livers and hearts of animals; (2) reacting protoporphyrin disodium, chlorhematin, hemoglobin or myoglobin which is taken as a substrate and a zinc ion solution under the catalysis of the Zn<2+> chelatase to obtain zinc porphyrin; and (3) and separating and purifying the zinc porphyrin by using high performance liquid chromatography. The method for preparing the natural haematochrome zinc porphyrin is simple and practicable; and the prepared zinc porphyrin is safe and stable, and has a good color generation effect.

Description

technical field [0001] The invention belongs to the field of food and relates to a preparation method of natural red pigment porphyrin zinc. Background technique [0002] Color is one of the important indicators affecting meat quality, and also one of the decisive factors affecting consumer purchasing behavior. The main red pigment in meat, that is, heme, is easily oxidized during processing and storage, and the color of the meat changes from bright red to brown, which greatly reduces the sensory quality of the meat. In order to make the meat products have a normal bright red color and improve the organizational structure of the products, at present domestic and foreign meat product enterprises mostly use nitrate or nitrite as the color developing agent of meat products to obtain ideal color and flavor. However, the use of nitrous acid exceeding a certain standard will lead to NO 2 - A large number of residues are combined with secondary amines of protein metabolites to f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18
Inventor 彭增起王园辛营营
Owner NANJING AGRICULTURAL UNIVERSITY
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