Method for effectively flowering and culturing impatiens balsamina through test tube
A culture method and test-tube flowering technology are applied in the field of plant tissue culture and can solve problems such as short growth period of impatiens
Inactive Publication Date: 2012-05-09
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology
The growth period of Impatiens is relatively short, and the flower color is brighter. At present, there are no reports about its flowering in test tubes.
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[0018] The technical contents of the present invention will be further described below in conjunction with the examples; the following examples are illustrative, not restrictive, and the protection scope of the present invention cannot be limited with the following examples.
[0019] A high-efficiency test-tube flowering cultivation method for Impatiens, the specific steps and materials are as follows:
[0020] 1.1 Materials
[0021] Seeds of Impatiens Celosia cristata were purchased from Caozhuang Flower Market.
[0022] 1.2 Treatment of seeds
[0023] Under sterile conditions, rinse the seeds of Impatiens with 75% alcohol for 30 seconds, soak them in 0.1% mercury liter aqueous solution for 10 minutes, then soak them with sterile water for 5-6 times, each time for about 2 minutes, and finally inoculate them on MS medium , the culture temperature is (25±2)°C, the light is 12-14h every day, and the light intensity is 1500-2000Ix. After 25 days, sterile seedlings with 3-4 tru...
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The invention discloses a method for effectively flowering and culturing impatiens balsamina through a test tube. The method comprises the following steps of: taking 1 / 8-1 / 2 MS as a basic culture medium, and adding 7.0 g / L of agar powder and 30 g / L of cane sugar into the culture medium, wherein the PH of the culture medium is 5.8; adding 6-BA with the concentration of 0.5-1.5 mg / L and 0.1-0.2 mg / L of NAA into the culture medium; adding active carbon with the concentration of 1-2g / L into the culture medium again, wherein the culture condition is as follows: illuminating for 12-14 h everyday below 25 DEG C, flowering and culturing under the illumination intensity of 1500-2000 Ix, and culturing for 75-75 d. According to the method disclosed by the invention, the cytokinin on the formation of flower buds has an important role; the NAA is cooperatively used, thus, the method has good facilitation on the flowering of the impatiens balsamina; the important role of the 6-BA and the NAA on the flowering is researched in a detailed manner; and the method has better directive function on action researches of other in stalk, premature, more in tiller and good in disease resistance and stress resistance.
Description
technical field [0001] The invention belongs to the field of plant tissue culture, in particular to a high-efficiency test-tube flowering culture method for Impatiens. Background technique [0002] Test tube flowering refers to the method of using tissue culture, so that the flowering process of plants is completed in a culture container. Since flower development is a complex physiological phenomenon, the whole development process can be divided into four stages: (1) flower induction: transition from vegetative growth to reproductive growth; (2) transition from induced state to meristem: inflorescence division (3) flower initiation: determination of floral organs; (4) morphogenesis of floral organs: formation and maturation of floral organs. Flower formation is a physiological phenomenon unique to higher plants. In most cases, plants must reach a certain maturity stage to start flower formation, and the use of test tube flowering can greatly shorten the time from seedlings...
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IPC IPC(8): A01H4/00
Inventor 黄俊轩李建科李双跃武春霞杨静慧刘艳军
Owner TIANJIN AGRICULTURE COLLEGE