Ruthenium (II)-polypyridine complex, and preparation method and application thereof

Abstract

The invention provides a ruthenium (II)-polypyridine complex. For the ruthenium (II)-polypyridine complex, bipyridyl (bpy) or phenanthroline hydrate (phen) is used as an ancillary ligand, and dipyridine[3,2-a:2',3'-c]phenazine-11,12-imidazole (dppzi) is used as a main ligand. The preparation method comprises the following steps: preparing 5,6-dinitrobenzimidazole from 5,6-nitrobenzimidazole; reducing 5,6-dinitrobenzimidazole into 5,6-diaminobenzimidazole by using hydrazine hydrate and palladium / carbon; preparing dppzi from phenanthroline hydrate-5,6-dione and 5,6-diaminobenzimidazole and preparing cis-[Ru(bpy)2Cl2].2H2O from ruthenium trichloride, bpy and lithium chloride; preparing cis-[Ru(phen)2Cl2].3H2O from ruthenium trichloride, phen and lithium chloride; carrying out a heating reflux reaction on dppzi and cis-[Ru(bpy)2Cl2].2H2O or cis-[Ru(phen)2Cl2].3H2O under the protection of argon, carrying out cooling, adding ammonium hexafluorophosphate, allowing precipitate to deposit and carrying out filtration and column chromatography so as to obtain a target product. The ruthenium (II)-polypyridine complex provided in the invention can be used as an optical switch for G-quadruplex DNA molecules.

Description

technical field [0001] The invention relates to metal complexes, in particular to a ruthenium (II) polypyridine complex and its preparation method and application. Background technique [0002] Ruthenium outermost layer has 4d 7 5s 1 The common valence states are Ru(I), Ru(II) and Ru(III), and it is easy to form six-coordinated complexes. Transition metal ruthenium (Ⅱ) polypyridine complexes have unique DNA bonding ability, good electrochemical, photochemical, photophysical properties and rich spectral properties, as well as low toxicity, easy absorption and excretion. It has a wide range of applications in DNA structure probes, DNA charge transfer, DNA footprint reagents, DNA fragmentation reagents and anticancer drugs, which has attracted widespread attention and has become a very active frontier and interdisciplinary field. [1] In particular, the discovery of DNA-molecular optical switch ruthenium complexes has attracted more attention. These complexes have no or weak...

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Problems solved by technology

[2,3] It is well known that [Ru(phen) 2 dppz] 2+ and [Ru(bpy) 2 dppz] 2+ It is widely recognized as an excellent DNA molecular optical switch complex. However, compared with a large number of studies on the bonding of complexes to double-stranded DNA, polypyridine ruthenium complexes as G-quadruplex DNA molecular optical switches are rarely reported.

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