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Aerobic denitrifying Paracoccus denitrificans and application thereof

A denitrification paracoccus, nitrification denitrification technology, applied in the direction of aerobic and anaerobic process treatment, bacteria, etc., can solve the problem that the total nitrogen removal rate is less than 50%, the performance has not been reported, and further improve it to overcome the treatment Efficiency and economical applicability, efficient and economical denitrification, and stable biological activity

Active Publication Date: 2013-03-20
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bacteria can degrade nitrate nitrogen from 282.0mg / L to 149.2mg / L within 5 days, and the removal rate of total nitrogen is less than 50%.
Zhu Yueqi (Study on the classification and characteristics of an aerobic denitrifying bacterium, Bulletin of Microbiology, 2009, 04) isolated an aerobic denitrifying bacterium CY1, which was initially identified as Paracoccus pantotrophus. Both have denitrification ability under anaerobic and aerobic conditions, the initial concentration of nitrate nitrogen is 137.25mg / L, and the removal rate of nitrate nitrogen within 30h is 99.98% (anaerobic) and 60.16% (aerobic), but the bacteria The performance of heterotrophic nitrification to ammonia nitrogen has not been reported, and it is limited in the treatment of ammonia nitrogen-containing sewage. In addition, the denitrification effect under aerobic conditions needs to be further improved.

Method used

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  • Aerobic denitrifying Paracoccus denitrificans and application thereof
  • Aerobic denitrifying Paracoccus denitrificans and application thereof
  • Aerobic denitrifying Paracoccus denitrificans and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Detection of the aerobic denitrification performance of Paracoccus denitrificans DN-3.

[0033] Preparation of simulated wastewater I (sodium succinate 4.3g, KNO 3 2.0g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, 1.0L water, pH7.0~7.5), the initial concentration of nitrate nitrogen is 277mg / L, and the mass ratio of carbon to nitrogen is 2.8. Treated for 26h under the conditions of dissolved oxygen (DO) of 0.5mg / L, temperature of 22℃ and pH of 7.0. The removal rate of nitrate nitrogen and total nitrogen is greater than 95%, and the removal rate of COD is greater than 90%.

[0034] Prepare simulated wastewater II (methanol 5.0mL, KNO 3 2.0g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, 1.0L water, pH7.0~7.5), the initial concentration of nitrate nitrogen is 277mg / L, and the mass ratio of carbon to nitrogen is 5.3. Treated for 29h under the conditions of DO 1.5mg / L, temperature 30℃ and pH8.0. Nitrate nitrogen removal rat...

Embodiment 2

[0036] Detection of heterotrophic nitrification-aerobic denitrification performance of Paracoccus denitrificans DN-3.

[0037] Prepare simulated wastewater III (4.3g sodium succinate, 1.25g ammonium sulfate, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, 1.0L water, pH 7.0-7.5), the initial concentration of ammonia nitrogen is 265mg / L, and the mass ratio of carbon to nitrogen is 2.9. Treated for 34h under the conditions of DO 0.5mg / L, temperature 25°C and pH7.5. The removal rate of ammonia nitrogen and total nitrogen is greater than 95%, and the removal rate of COD is greater than 90%.

[0038] Prepare simulated wastewater IV (methanol 5.0mL, ammonium sulfate 1.25g, KH 2 PO 4 1.0g, FeCl 2 ·H 2 O 0.5g, CaCl 2 ·H 2 O 0.2g, MgSO 4 1.0g, 1.0L water, pH 7.0-7.5), the initial concentration of ammonia nitrogen is 265mg / L, and the mass ratio of carbon to nitrogen is 5.6. Incubate for 36h under the conditions of DO 2.0mg / L, temperature 35℃ and pH8.5. The removal ...

Embodiment 3

[0040] Paracoccus denitrificans DN-3 is used in MSG wastewater with high COD concentration.

[0041] The quality of MSG wastewater: the concentration of ammonia nitrogen is 300mg / L~450mg / L, and the COD is 700mg / L~850mg / L. First, the nitrifying bacteria cultivated in our laboratory are used to convert ammonia nitrogen into nitrate nitrogen, and the nitrification effluent is used as denitrification feed water. Then, DN-3 is used for aerobic denitrification. When the carbon source is insufficient, methanol is added to make the carbon-nitrogen mass ratio 3.0. The initial nitrate nitrogen concentration is 280mg / L, and the COD is 720mg / L. After treatment for 32 hours at a DO of 0.5 mg / L, a temperature of 30°C and a pH of 7.0 to 8.5, the removal rate of nitrate nitrogen and total nitrogen was greater than 90%, and the removal rate of COD was greater than 80%.

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Abstract

The invention relates to aerobic denitrifying paracoccus denitrificans and application thereof. A bacterial strain provided in the invention is paracoccus denitrificans DN-3CGMCC No. 3658; the Paracoccus denitrificans can carry out aerobic denitrification by using nitrate nitrogen under both aerobic and oxygen-limited conditions and can also carry out heterotrophic nitrification-aerobic denitrification by using ammonia-N, and a total nitrogen removal rate on above mentioned occasions is greater than 90%. The paracoccus denitrificans provided in the invention has stable hereditary features, is applicable to treatment of a variety of nitrogen-containing waste water and produces a good nitrogen removal effect.

Description

Technical field [0001] The invention relates to the field of environmental microorganisms. Specifically, it relates to a strain of Paracoccus denitrificans for biological denitrification and its application. The Paracoccus denitrification of the invention has strong denitrification performance under aerobic conditions, and is particularly suitable for simultaneous nitrification and denitrification of ammonia-containing wastewater. Background technique [0002] With the increasingly serious nitrogen pollution in water bodies, nitrogen removal has become the focus and hot spot in the current water treatment research. At present, it is generally believed that biological nitrogen removal is one of the more economical and effective methods to remove nitrogen pollution from wastewater. Traditional biological denitrification includes two processes: aerobic nitrification and anaerobic denitrification. Nitrification means that autotrophic nitrifying bacteria oxidize ammonia nitrogen to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/30
Inventor 李志瑞高会杰黎元生许谦刘忠生
Owner CHINA PETROLEUM & CHEM CORP
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