Method for producing phospholipid

一种制造方法、磷脂的技术，应用在磷脂的制造领域，能够解决难酯化反应等问题，达到低成本的效果

Active Publication Date: 2012-05-23
KANEKA CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Then, it has been reported that in the esterification reaction caused by phospholipase A2, when immobilized phospholipase A2 is used, it is difficult to efficiently carry out the esterification reaction even if a large amount of fatty acid is used relative to lysophospholipid (non-patent literature 2. Non-patent literature 3)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] To 35 mg of lysolecithin ("SLP-LPC70" manufactured by Tsuji-Seiyu Co., Ltd.), 105 mg of DHA-containing fatty acid prepared by hydrolyzing DHA-50G (manufactured by Nippon Chemical Feed Co., Ltd.; Glycerin (manufactured by Sakamoto Pharmaceutical Co., Ltd.) was 1 g, and then, 37.5 mg of glycine (manufactured by Showa Denko) and 37.5 mg of alanine (manufactured by Musashino Chemical Laboratory Co., Ltd.) were added. Then, 20 mg of phospholipase A2 ("Powder Lysonase", 53 U / mg manufactured by Sanyo Fine Co., Ltd.) was added, and after depressurization at 0.6 torr (80 Pa) for 10 minutes to remove moisture, 0.3 mol / l calcium chloride (Tomita Pharmaceutical Co., Ltd.) was added. (manufactured by the company) solution 10 μl and water 30 μl were reacted at 50° C. for 24 hours. The content of DHA in the phospholipids and lysophospholipids contained in the reaction solution was 13.4% by weight.

[0070] After adding 1 ml of ethanol to the reaction solution and stirring, extraction...

Embodiment 2

[0073] To 35 mg of lysolecithin ("SLP-LPC70" manufactured by Tsuji-Seiyu Co., Ltd.), 30 mg of DHA-containing fatty acid prepared by hydrolyzing Incromega DHA-J46 (manufactured by Croda Japan, DHA content: 49.7% by weight) by a conventional method, Glycerin (manufactured by Sakamoto Pharmaceutical Co., Ltd.) was 1 g, and then, 25 mg of glycine (manufactured by Showa Denko) and 25 mg of alanine (manufactured by Musashino Chemical Laboratory Co., Ltd.) were added. Then, 20 mg of phospholipase A2 ("Powder Lysonase", 53 U / mg manufactured by Sanyo Fine Co., Ltd.) was added, and after depressurization at 0.6 torr (80 Pa) for 10 minutes to remove moisture, 0.3 mol / l calcium chloride (Tomita Pharmaceutical Co., Ltd.) was added. 10 µl of the solution (manufactured by the company) was reacted at 50° C. for 24 hours while reducing pressure at 50 torr (6.7 kPa). The content of DHA in the phospholipids and lysophospholipids contained in the reaction solution was 16.9% by weight.

[0074] T...

Embodiment 3

[0077] To 50 mg of lysolecithin ("SLP-White Lyso" manufactured by Tsuji-Seiyu Co., Ltd.), 30 mg of DHA-containing fatty acid prepared by hydrolyzing Incromega DHA-J46 (manufactured by Croda Japan Co., Ltd., DHA content: 49.7% by weight) in a conventional method was added 1 g of glycerin (manufactured by Sakamoto Pharmaceutical Co., Ltd.), and then, 25 mg of glycine (manufactured by Showa Denko) and 25 mg of alanine (manufactured by Musashino Chemical Laboratory Co., Ltd.) were added. Then, 20 mg of phospholipase A2 ("Powder Lysonase", 53 U / mg manufactured by Sanyo Fine Co., Ltd.) was added, and after depressurization at 0.6 torr (80 Pa) for 10 minutes to remove moisture, 0.3 mol / l calcium chloride (Tomita Pharmaceutical Co., Ltd.) was added. 10 µl of the solution (manufactured by the company) was reacted at 50° C. for 24 hours while reducing pressure at 50 torr (6.7 kPa). The content of DHA in the phospholipids and lysophospholipids contained in the reaction solution was 15.3%...

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PUM

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Abstract

Provided is a method for producing a phospholipid at a low cost by reusing phospholipase A2 in a method for producing the phospholipid whereby an arbitrary fatty acid is bonded to the 2-position of a phospholipid using an esterification reaction catalyzed by phospholipase A2 in glycerol. The method for producing a phospholipid is characterized by comprising conducting an esterification reaction catalyzed by phospholipase A2 between a lysophospholipid and an acyl donor in glycerol to form a phospholipid, adding a solvent which is immiscible with glycerol to form a glycerol layer and a solvent layer, extracting said phospholipid into said solvent layer, allowing phospholipase A2 to migrate into said glycerol layer, and, after separating the glycerol layer and distilling off the solvent remaining therein, further adding to the residual glycerol solution the lysophospholipid and the acyl donor to thereby conduct the esterification reaction again with the use of phospholipase A2 remaining in said glycerol solution.

Description

technical field [0001] The present invention relates to a method for producing phospholipids, in particular to a method for producing phospholipids using phospholipase A2. Background technique [0002] According to research on lipids in recent years, it has been found that highly unsaturated fatty acids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) can improve learning functions, prevent arteriosclerosis, and improve lipids. Metabolic functions and other functions. Especially DHA, it has been found that by ingesting it in a form bonded to a phospholipid such as lecithin, it can obtain stronger antioxidant properties than the triglyceride type, can improve stability, and is well absorbed, so it is easy to express The physiological activity that DHA has. Functional fatty acids other than DHA, such as EPA, conjugated linoleic acid, and arachidonic acid, can also be expected to improve their physiological activity by bonding to phospholipids. [0003] Met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11B11/00C11C3/10C12P9/00
CPCC12P7/6481C11C3/10A23D9/013C12P7/6472
Inventor 田中立志岩崎雄吾
Owner KANEKA CORP
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