Preparation method of soybean protein emulsion with high emulsibility
A soybean protein and emulsifying technology, applied in vegetable protein processing and other directions, can solve the problems of insufficient phospholipid effect, difficult to control processing and storage, poor stability of the emulsifying system, etc., and achieve good emulsifying activity and stability, emulsifying activity and Improved stability and strong emulsification stability
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[0012] Weigh 10g of soybean lecithin, dissolve it in Tris-HCl buffer solution to obtain soybean lecithin solution, then add CaCl2 to make the calcium ion concentration 150mM, preheat the soybean lecithin solution at 65°C for 5min, then add 0.3 g Phospholipase A2 was subjected to enzymolysis for 120 minutes at a temperature of 65°C. After enzymolysis, the enzyme was inactivated at 90°C for 10 minutes. After enzymolysis, the soybean lecithin solution was freeze-dried to obtain hydrolyzed soybean lecithin powder. In the phosphate buffer solution of pH=7, stir magnetically for 1 hour until completely dissolved to obtain a hydrolyzed soybean lecithin solution; dissolve soybean protein isolate in a phosphate buffer solution of pH=7 and magnetically stir for 1 hour until completely dissolved to obtain a soybean protein solution ; The hydrolyzed soybean lecithin solution and the soybean protein solution were mixed according to the solid mass ratio of 8:1 and then magnetically stirred f...
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