Core-shell nano particle for covering plant extractive and preparation method thereof
A plant extract, nanoparticle technology, applied in the preparation of microspheres, microcapsule preparations, etc., can solve problems such as insolubility, and achieve the effects of small particle diameter, delayed release, and easy water storage.
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Embodiment 1
[0068] Step 1. Proportioning the following raw materials: 0.1mg Maca, 2mg PLA with a molecular weight of 100,000;
[0069] Step 2, dissolving the Maca and PLA in step 1 in 1ml acetocyanide to obtain acetocyanide solution A;
[0070] Step 3, 0.24mg soybean lecithin and 0.06mg DSPE-PEG-COOH were dissolved in 3ml 4% ethanol aqueous solution to obtain ethanol aqueous solution B;
[0071] Step 4. Under an ice bath, pipette the acetocyanide solution A obtained in step 2, and add it dropwise to the ethanol aqueous solution B obtained in step 3. Use probe-type ultrasonic ultrasound for 5 minutes of continuous ultrasonication, and use 10KDa ultrafiltration After the tube was ultrafiltered three times to remove organic solvents and free molecules, the polylactic acid-phospholipid-polyethylene glycol core-shell nanoparticles coated with Maca were obtained.
Embodiment 2
[0073] Step 1. Proportioning the following raw materials: 1mg Maca, 2mg PLA with a molecular weight of 100,000;
[0074] Step 2, dissolving the Maca and PLA in step 1 in 1ml acetocyanide to obtain acetocyanide solution A;
[0075] Step 3, 0.24mg soybean lecithin and 0.06mg DSPE-PEG-COOH were dissolved in 3ml 4% ethanol aqueous solution to obtain ethanol aqueous solution B;
[0076] Step 4. Under an ice bath, pipette the acetocyanide solution A obtained in step 2, and add it dropwise to the ethanol aqueous solution B obtained in step 3. Use probe-type ultrasonic ultrasound for 5 minutes of continuous ultrasonication, and use 10KDa ultrafiltration After the tube was ultrafiltered three times to remove organic solvents and free molecules, the polylactic acid-phospholipid-polyethylene glycol core-shell nanoparticles coated with Maca were obtained.
Embodiment 3
[0078] Step 1. Proportioning the following raw materials: 0.1mg Maca, 2mg PLA with a molecular weight of 100,000;
[0079] Step 2, dissolving the Maca and PLA in step 1 in 1ml acetocyanide to obtain acetocyanide solution A;
[0080] Step 3, 0.24mg soybean lecithin and 0.06mg DSPE-PEG-COOH were dissolved in 1.5ml 4% ethanol aqueous solution to obtain ethanol aqueous solution B;
[0081] Step 4. Under an ice bath, pipette the acetocyanide solution A obtained in step 2, and add it dropwise to the ethanol aqueous solution B obtained in step 3. Use probe-type ultrasonic ultrasound for 5 minutes of continuous ultrasonication, and use 10KDa ultrafiltration After the tube was ultrafiltered three times to remove organic solvents and free molecules, the polylactic acid-phospholipid-polyethylene glycol core-shell nanoparticles coated with Maca were obtained.
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