Extraction method of stevioside

An extraction method and technology of stevioside, which are applied in the field of stevioside extraction, can solve the problems of complicated procedures, low degree of mechanization, difficult to implement seed production area, etc., to expand production scale, improve production efficiency, and reduce labor costs. Effect

Inactive Publication Date: 2012-06-20
安徽方敏医药科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the rapid development of the rural economy, the seed production bases in various provinces in my country have to varying degrees faced the practical problems of labor shortage, increased seed production costs, and difficult implementation of seed production areas, which seriously restricted the construction of seed bases and the development of the seed industry.
The main reasons are as follows: First: The seed production technology is complex and the degree of mechanization is low. The current hybrid rice seed production technology in my country is a labor-intensive technology that must first plant the male parent, and then plant the female parent after a period of time. From sowing to harvesting is completely dependent on manpower Manual operation, complex procedures, the entire seed production process requires a lot of labor, and the seed production base can only be selected in rural areas with underdeveloped economies and abundant labor
However, in recent years, a large number of young and middle-aged laborers in seed production bases have gone out to work in cities, causing most of the bases to be unable to carry out seed production due to the lack of necessary labor force; second: state-owned large-scale farms cannot be carried out, because the current hybrid rice seed production technology cannot be carried out Mechanical operation, economically developed areas with a high degree of mechanization and state-owned large-scale farms are difficult to produce hybrid rice seeds because of the lack of necessary labor; third: it is difficult to ensure the quantity and quality of seeds, because the current technology is one family. Difficulty securing the quantity and quality of seeds needed for food production
seriously affected the rice industry and food security

Method used

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  • Extraction method of stevioside
  • Extraction method of stevioside
  • Extraction method of stevioside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Select Lv3A, Lv102S, etc. with good flowering habits as the female parent, and the MC526 restorer line containing bendazone-sensitive genes as the male parent. Mix evenly, dry seeding with wheat planter or water direct seeding with rice seeder, or evenly spread artificially. The field management before heading is in accordance with normal management, spray benta pine at the heading stage, catch powder with a fan of a motorized sprayer at the heading stage, when mature, the male parent dies, and the female parent grows normally, and mechanical harvesting is carried out.

[0022] Restorer line cultivation: first select MC526 containing bendazone-sensitive gene as one of the hybrid parents, and then select another parent such as 9311 with high quality, disease resistance and lodging resistance for hybridization, F2 generation is selected for pentazone, and subsequent generations are selected A single plant with excellent traits will be kept until the traits are ...

Embodiment 2

[0023] Embodiment 2: select good green three A, 102S etc. of flowering habit as female parent, 9Z013, 9Z127 etc. are male parent, select improved wheat planter or improved paddy direct seeding machine for use, the male parent's seed is adorned in the first seed box on the left, later The female parent in the 2nd-6th seed box, from left to right, S-shaped operation, the distance between the first opener and the second opener is 30 cm, and the distance between the 2nd-6 openers 15 cm. After the pollination ends, the male parent is cut down with a portable 430 side-mounted rice harvester, and harvested with a large harvester when mature.

[0024] The 9Z013 described above is a restorer line with stable traits successfully selected and bred by crossing the American naked rice as the female parent and 9311 as the male parent. It has the characteristics of lodging resistance, high quality, high yield and large pollen amount.

[0025] The 9Z127 described above is a restorer line wit...

Embodiment 3

[0027] Example 3: Transfer the rice herbicide-resistant gene to the rice male sterile line through sexual hybridization, cultivate the herbicide-resistant sterile line, and then select the restorer line with the same initial sowing period, according to the ratio of 1:5 before sowing Mixing, dry direct seeding with wheat seeder or water direct seeding with rice direct seeding machine, or manual even broadcasting. The field management before heading is in accordance with normal management. During the heading stage, the fan of the motorized sprayer is used to catch the powder. When it is mature, spray "glufosinate" to kill the male parent. The female parent grows normally and is harvested mechanically.

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Abstract

The invention discloses an extraction method of stevioside; firstly an excellent sterile line and a restorer line with a similar growth period are cultured, and then a hybrid rice combination which is suitable for mechanized seed production and has strong heterosis is bred; parents of the hybrid combination are used as test carriers; through systemic research, related genes are implanted in the sterile line or the restorer line; the male parent is killed and the female parent is harvested after pollination; by the above method, a brand new hybrid rice seed production technical system suitable for mechanized operations is constructed; the technical bottleneck problem of hybrid rice seed production is solved; mechanized production is realized; the production is enlarged, large-scale and intensive; the production efficiency is increased; and the labor cost is reduced.

Description

technical field [0001] The invention relates to a method for extracting stevioside, in particular to a method for extracting stevioside Background technique [0002] The annual production area of ​​hybrid rice in my country is about 16 million hectares, requiring about 240 million kilograms of seeds, and the seed production area is about 150,000 hectares, mostly distributed in rural areas in economically underdeveloped areas. In recent years, with the rapid development of the rural economy, the seed production bases in various provinces in my country have to varying degrees faced the practical problems of labor shortage, increased seed production costs, and difficult implementation of seed production areas, which seriously restricted the construction of seed bases and the development of the seed industry. . The main reasons are as follows: First: The seed production technology is complex and the degree of mechanization is low. The current hybrid rice seed production technolo...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor方敏吴启中
Owner安徽方敏医药科技股份有限公司