Lipopetide biosurfactant oil extraction agent for improving extraction rate of crude oil

A surfactant and bio-lipid technology, applied in microorganisms, drilling compositions, microorganism-based methods, etc., can solve the problems of low recovery rate, serious pollution, high cost of chemical oil recovery agents, and improve rock wetting. Excellent properties, excellent performance, and the effect of reducing interfacial tension

Active Publication Date: 2013-09-25
DAQING HUALI ENERGY BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims at overcoming the deficiencies of the prior art such as high cost of chemical oil recovery agents, serious pollution, low enhanced oil recovery, etc., and provides a biological lipopeptide surfactant oil recovery agent for enhanced crude oil recovery

Method used

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  • Lipopetide biosurfactant oil extraction agent for improving extraction rate of crude oil
  • Lipopetide biosurfactant oil extraction agent for improving extraction rate of crude oil
  • Lipopetide biosurfactant oil extraction agent for improving extraction rate of crude oil

Examples

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Effect test

Embodiment 1

[0024] A biological lipopeptide surfactant oil recovery agent for improving crude oil recovery of the present invention: the lipopeptide surfactant-producing bacteria Bacillus subtilis ACCC01430 is fermented to obtain a fermented liquid, and the fermented liquid and the nutrient liquid are divided into 3: After mixing at a volume ratio of 1, add a weak base of sodium carbonate to adjust the pH to 8-9, and then make a biolipopeptide surfactant oil displacement agent;

[0025] The nutrient solution is 1% of molasses, 1% of urea, 0.5% of peptone, 0.2% of yeast extract, 0.3% of diammonium hydrogen phosphate, and the balance is water.

Embodiment 2

[0027] A biological lipopeptide surfactant oil recovery agent for improving crude oil recovery of the present invention: the lipopeptide surfactant-producing bacterium Bacillus subtilis ACCC01430 is fermented to obtain a fermented liquid, and the fermented liquid and the nutrient liquid are mixed according to 5: After mixing at a volume ratio of 1, add a weak base of sodium carbonate to adjust the pH to 8-9, and then make a biolipopeptide surfactant oil displacement agent;

[0028] The nutrient solution is 1% of molasses, 1% of urea, 0.5% of peptone, 0.2% of yeast extract, 0.3% of diammonium hydrogen phosphate, and the balance is water.

Embodiment 3

[0030] A biological lipopeptide surfactant oil recovery agent for improving crude oil recovery of the present invention: the lipopeptide surfactant-producing bacterium Bacillus subtilis ACCC01430 is fermented to obtain a fermented liquid, and the fermented liquid and the nutrient liquid are divided into 4: 1 volume ratio, add weak alkali sodium carbonate to adjust the pH to 8-9, and then make the biological lipopeptide surfactant oil displacing agent;

[0031] The nutrient solution is 1% of molasses, 1% of urea, 0.5% of peptone, 0.2% of yeast extract, 0.3% of diammonium hydrogen phosphate, and the balance is water.

[0032] The title and strain source of the bacterium that the present invention adopts are as follows:

[0033]Bacillus subtilis (ACCC01430), China Agricultural Microbiology Culture Collection Center.

[0034] For the standard of the decline period of the thalline in Examples 1 to 4 of the present invention, see the second edition of "Microbiology Tutorial" edited...

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Abstract

The invention provides a lipopetide biosurfactant oil extraction agent for improving extraction rate of crude oil and relates to the technical field of microbial oil extraction. The lipopetide biosurfactant oil extraction agent is prepared by obtaining fermentation liquor after fermentation by lipopetide-biosurfactant-producing bacillus subtilis ACCC01430 and mixing the fermentation liquor and a nutrient solution according to a volume ratio of (3-5):1, wherein the nutrient solution comprises 1 percent of molasses, 1 percent of urea, 0.5 percent of peptone, 0.2 percent of yeast extract, 0.3 percent of ammonium monohydric phosphate and the balance of water; and the fermentation liquor is prepared by fermenting with bacillus subtilis ACCC01430 by a method specified in the description. According to the lipopetide biosurfactant oil extraction agent for improving extraction rate of crude oil, a large amount of lipopetide surfactant is produced by ground fermentation; the surfactant can emulsify crude oil, improve wettability of rocks, reduce oil-water interface tension and reduce viscosity of the crude oil; and the bacteria can reproduce and grow in oil layer at 50 DEG C. The nutrient solution can promote the growth of the bacteria and activate microbes to extract oil.

Description

technical field [0001] The invention relates to the technical field of microbial oil recovery, in particular to a biological lipopeptide surfactant oil recovery agent for enhancing crude oil recovery. Background technique [0002] From a broad perspective, the basic methods of microbial enhanced oil recovery technology mainly include two categories: one is produced on the ground through industrial fermentation, and the useful metabolites are separated from the fermentation liquid and then injected into the oil reservoir; It is usually also called on site or at surface, or surface method for short; the other is to use the oil reservoir as a huge bioreactor to allow microorganisms to ferment in the underground oil layer, which is usually called underground fermentation method (in situ), referred to as the reservoir method. [0003] Microbial underground fermentation (reservoir method) uses the oil reservoir as a huge bioreactor, injects selected microorganisms with good compa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K8/584C12R1/125
Inventor 沈玉江孙彦达李国军王柱张丽茹段宝颜
Owner DAQING HUALI ENERGY BIOLOGICAL TECH
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