Valproic acid homogeneous-phase enzyme immunity rapid detection kit

A detection kit and homogeneous enzyme immunization technology, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as unfavorable promotion and insufficient accuracy, and meet the requirements of low detection equipment, good accuracy, High specificity and sensitivity

Inactive Publication Date: 2012-06-20
SICHUAN KINGMED DIAGNOSTICS CENT
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Problems solved by technology

ELISA detection method has high sensitivity, but insufficient accuracy, only semi-quantitative
At the same time, there is no self-developed luminescent immune reagent in China, and the detection is mainly carried out on a closed luminometer by purchasing a foreign analysis kit.
Limited by the cost of instruments and reagents, this method is not conducive to the nationwide promotion

Method used

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Embodiment Construction

[0023] The present invention establishes the detection method of valproic acid based on homogeneous enzyme immunoassay, and its principle mainly uses the principle of competitive immune reaction, and the reaction process is the competitive combination of free valproic acid in liquid samples and valproic acid coupled on G6PDH specific antibody. The G6PDH-valproic acid conjugate has immune reaction and enzyme catalytic activity, can catalyze redox reactions, and convert nicotinamide adenine dinucleotide (NAD) into NADH. This process results in a change in absorbance, which can be detected under a 340nm wavelength light source. Once the conjugate is combined with the antibody, the enzyme catalytic activity of the conjugate is inhibited, so the more valproic acid in the liquid sample, the more G6PDH-valproic acid conjugate is released, resulting in an increase in the absorption value.

[0024] In order to describe the technical content, structural features, achieved goals and eff...

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Abstract

The invention relates to the technical field of valproic acid blood drug level monitoring, and specifically discloses a valproic acid homogeneous-phase enzyme immunity rapid detection kit. With derivative synthesis, valproic acid is connected onto bovine serum albumin (BSA), such that an immunizing antigen is obtained. An obtained polyclonal antibody has valproic acid specificity. Experiment data shows that when the reagent is used for detecting valproic acid, no cross reaction occurs between the reagent and other commonly used medicines; an analysis sensitivity is better than 25ng/ml; within-run precision and within-run precision coefficient of variability (CV) are both smaller than 4.7%; and compared with a fluorescence polarization immunoassay (EPIA) method, an R2 value reaches 0.98. The kit can be used in various routine analysis instruments. Because of characteristics of simple operation, high speed, low cost, and high sensitivity, with the kit, automatic detection can easily be realized. The kit assists in guiding clinical customized medication. With the kit, toxic or side effect of medicines is reduced, and curative effect optimization is realized.

Description

technical field [0001] The invention belongs to the technical field of detection of valproic acid clinical samples, and in particular relates to a kit for rapidly detecting the concentration of valproic acid. technical background [0002] Among the many antiepileptic drugs currently available, valproic acid is the first choice and broad-spectrum drug with wide clinical application, high curative effect and good safety. Valproic acid can be used not only for the treatment of generalized seizures of various types of epilepsy, especially absence seizures and tonic-clonic seizures, but also for partial seizures. In addition, valproic acid rarely affected cognitive function in patients and rarely caused seizure exacerbations. Due to the differences in pharmacokinetics of individual patients during treatment, the correlation between the dose of valproic acid and the plasma concentration is poor. Usually 50% of the blood drug concentration measured by conventional dose administra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/535
Inventor 虞留明王金文王文峰陈美才梁耀铭
Owner SICHUAN KINGMED DIAGNOSTICS CENT
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