Method for extracting asparagus polysaccharides from Radix asparagi
A technology of aspartame polysaccharide and aspartame, which is applied in the field of extracting aspartame polysaccharide from asparagus, can solve the problems of many impurities in crude polysaccharide, affecting the medicinal effect of aspartame polysaccharide, etc., so as to improve the total sugar content, ensure the activity, improve the The effect of extraction rate
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Embodiment 1
[0021] Wash and dry 100kg of asparagus that has passed the inspection; put it into a pulverizer and crush it, pass through a 0.5mm sieve; then add the powder to 2100kg of distilled water, and extract it twice at 77°C with a frequency of 30kHz ultrasonic waves, each time for 30 minutes Then combine twice filtrate, concentrate under reduced pressure to 150kg on the rotary evaporator; Then adjust its pH value to 3 with 10% trichloroacetic acid, place overnight, centrifuge 5 minutes under the condition of 3000r / min, discard the precipitate, and then Add 0.1% activated carbon to the supernatant to decolorize twice; then add 4 times the amount of absolute ethanol to the decolorized solution for precipitation, leave it overnight, and then use suction filtration to separate the alcohol precipitation solution from solid to liquid, and keep the precipitate; then The precipitate was washed twice with ethanol and acetone, and finally the precipitate was placed in a drying tray and dried in...
Embodiment 2
[0024] Wash and dry 100kg of asparagus that has passed the inspection; put it into a pulverizer, crush it, and pass through a 0.5mm sieve; then add the powder to 2200kg of distilled water, and extract it twice at a temperature of 78°C with a frequency of 33kHz ultrasonic waves, each time for 30 minutes Then combine twice filtrate, concentrate under reduced pressure to 150kg on the rotary evaporator; Then adjust its pH value to 3 with 10% trichloroacetic acid, place overnight, centrifuge 5 minutes under the condition of 3000r / min, discard the precipitate, and then Add 0.1% activated carbon to the supernatant to decolorize twice; then add 4 times the amount of absolute ethanol to the decolorized solution for precipitation, leave it overnight, and then use suction filtration to separate the alcohol precipitation solution from solid to liquid, and keep the precipitate; then The precipitate was washed twice with ethanol and acetone, and finally the precipitate was placed in a drying...
Embodiment 3
[0027] Wash and dry 100kg of asparagus that has passed the inspection; put it into a pulverizer and crush it, pass through a 0.5mm sieve; then add the powder to 2300kg of distilled water, and extract it twice at a temperature of 79°C with a frequency of 35kHz ultrasonic waves, each time for 30 minutes Then combine twice filtrate, concentrate under reduced pressure to 150kg on the rotary evaporator; Then adjust its pH value to 3 with 10% trichloroacetic acid, place overnight, centrifuge 5 minutes under the condition of 3000r / min, discard the precipitate, and then Add 0.1% activated carbon to the supernatant to decolorize twice; then add 4 times the amount of absolute ethanol to the decolorized solution for precipitation, leave it overnight, and then use suction filtration to separate the alcohol precipitation solution from solid to liquid, and keep the precipitate; then separate The precipitate was washed twice with ethanol and acetone, and finally the precipitate was placed in ...
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