Method for culturing strain of Agaricus campestris and Agaricus blazei using white loam soil as filter layer

Abstract

The invention discloses a method for culturing a strain of Agaricus campestris and Agaricus blazei using white loam soil as filter layer, which can be applied to grain stock or grain cultivar culture. The invention is characterized in that the method for culturing grain stock or grain cultivar comprises the following steps of: preparing a grain culture medium and sufficient natural white loam soil; adjusting the water content of white loam soil to 24-27% and pH to 7.3-7.7; loading the grain culture medium to bottles, adding the white loam soil to the bottles such that the white loam soil is located above the grain culture medium and keeps 4-5 cm away from the bottle opening and the thickness of white loam soil is 1.5-2 cm; sterilizing and then inoculating; and putting the culture bottles in a culture room and culturing stock or cultivar by the routine method. The method can filter off foreign microbes to minimize contamination of foreign microbe during the culture process, so as to increase the yield up to 99-100%. The filter layer is easily available and has a low cost.

Description

(1) Technical field [0001] The invention relates to a technology for producing edible fungus strains, in particular to a method for preparing mushroom and Agaricus blazei strains by using white loam as a filter layer. (two), background technology [0002] As we all know, the key prerequisite for the success of artificial cultivation of edible fungi such as mushrooms and Agaricus blazei is the strain. Only excellent strains can achieve high quality and high yield. , that is to purify and rejuvenate from the fruiting body to obtain the mother species (also known as the first-class species); the second stage is to produce the original species, that is, to multiply the mother species to the original species, that is, to use the mycelium of the mother species The body is inserted into the original seed culture medium, expanded and propagated and cultivated into the original seed (secondary species); the third stage is to produce the cultivated species, and it is not enough to hav...

AI Technical Summary

Problems solved by technology

Because the fermentation material itself is sterile, after the bacteria are inserted, the mycelia will quickly cover the upper part of the entire bottle while eating the material, and it is easy to form a co-growth advantage to inhibit miscellaneous bacteria. This method can greatly reduce the pollution rate , the disadvantage is that the operation is complicated, time-consuming and labor-intensive

[0005] Although using the above-mentioned measures to reduce the contamination rate of bacterial strains has a good effect of improving the yield of bacterial strains in general, due to the traditional production of original species (secondary species) and cultivated species (third-level species) such as mushrooms and Agaricus blazei In the process of seeding), fermented rice straw or cotton husks or cow dung and other fermented materials that are easy to retain moisture are used as the inoculation block of the mother seed or the original seed to form a mycelium culture substrate after germination, and these mycelium culture substrates are used as guides. Primer (Note: These inoculation block materials that form mycelium culture substrate are often referred to as guide material), which will redirect mycelium from the original inoculation block to the new cultivation substrate to facilitate the formation of co-growth advantages (note : as the material of guide material generally all is the fermented material that some nutrition is rich and has the raw material of good moisturizing ability to make through special fermentation to form co-growth advantage favorably), as can be seen from the above, due to large-scale bacteria The production process of the species, especially in the inoculation process, is difficult to achieve a true aseptic operation state, which has a great negative impact on the common practice of using the co-growth advantage of the strain hyphae to inhibit the growth of miscellaneous bacteria, not only reducing the bacteria The pollution rate is limited and the operation is cumbersome

[0006] In addition, with the development of science, traditional dung grass strains (also known as dung grass cultivars) used for edible fungi such as mushrooms and Agaricus blazei have been on the verge of being eliminated, and the next one is to make mushrooms and Agaricus blazei. As the original species and cultivars for production, wheat grain strains emerged as the times require. Edible fungus such as mushrooms and Agaricus blazei have short production cycle, simple process, consistent bacterial age, strong stress resistance, convenient sowing, and not easy to age. It is more resistant to storage and transportation, so it is more important to use the above-mentioned measures to reduce the pollution rate when making wheat grain strains, but it is also difficult to achieve true aseptic operation in the production process, and often due to guidance The material is rich in nutrients, and the mycelium of the miscellaneous bacteria also eats the material and grows very quickly, thus greatly weakening the original co-growth advantage. The rate is low, which seriously restricts and affects the production efficiency