Microbial gelled material and method for forming magnesite by using same to glue sand grains

A technology of microbial glue and magnesite, which is applied in the field of magnesite cemented sand particles, can solve the problems of polluting the environment and destroying the ecology, and achieves the effect of clean environment and low cost

Inactive Publication Date: 2012-07-04
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Except for epoxy resin, the rest of these materials have the e

Method used

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  • Microbial gelled material and method for forming magnesite by using same to glue sand grains
  • Microbial gelled material and method for forming magnesite by using same to glue sand grains
  • Microbial gelled material and method for forming magnesite by using same to glue sand grains

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Weigh 5g of peptone, 3g of beef extract and 1000ml of distilled water to prepare a medium, adjust the pH to 7.0, sterilize and dry, inoculate the prepared medium with Bacillus pasteurianus, and shake at 30°C Cultivate with an oscillation frequency of 170r / min, and cultivate for 24h;

[0030] (2) Configure the concentration of urea solution to be 2 mol L -1 , MgCl 2 ·6H 2 O solution concentration is 2mol L -1 mixed solution;

[0031] (3) Weigh 56.56g of sand with a particle size below 0.15mm and 23.44g of sand with a particle size of 0.15~0.3mm, and put them into a 60ml disposable syringe in three times, and tap the outside of the syringe during each loading process to The loaded sand is densely packed, and the tight void ratio is 43.1%;

[0032] (4) Connect the bottom of the syringe with a peristaltic pump, inject the bacterial solution at a rate of 5ml / min, let it stand for 2 hours after filling, and inject the mixed solution at a rate of 15ml / min after the ba...

Embodiment 2

[0037] (1) Weigh 4g of peptone, 4g of beef extract and 1000ml of distilled water to prepare a medium, adjust the pH to 6.0, sterilize and dry, inoculate Bacillus pasteurianus into the prepared medium, and shake at 30°C Cultivate with an oscillation frequency of 170r / min, and cultivate for 16h;

[0038] (2) Configure the concentration of urea solution to be 3 mol L -1 , MgCl 2 ·6H 2 O solution concentration is 1mol L -1 mixed solution;

[0039] (3) Weigh 63.63g of sand with a particle size below 0.15mm and 26.37g of sand with a particle size of 0.15~0.3mm, and put them into a 60ml disposable syringe in three times, and tap the outside of the syringe during each loading process to The loaded sand is densely packed, and the tight void ratio is 42.8%;

[0040] (4) Connect the bottom of the syringe with a peristaltic pump, inject the bacterial solution at a rate of 4ml / min, let it stand for 1 hour after filling, and inject the mixed solution at a rate of 10ml / min after the bac...

Embodiment 3

[0042] (1) Weigh 6g of peptone, 4g of beef extract and 1000ml of distilled water to prepare a medium, adjust the pH to 8.0, sterilize and dry, inoculate the prepared medium with Bacillus pasteurianus, and shake at 30°C Cultivate with an oscillation frequency of 170r / min, and cultivate for 24h;

[0043] (2) Configure the concentration of urea solution to be 1 mol L -1 , MgCl 2 ·6H 2 O solution concentration is 3mol L -1 mixed solution;

[0044] (3) Weigh 49.49g of sand with a particle size below 0.15mm and 20.51g of sand with a particle size of 0.15~0.3mm, and put them into a 60ml disposable syringe in three times, and tap the outside of the syringe during each loading process to The loaded sand is densely packed, and the tight void ratio is 42.6%;

[0045] (4) Connect the bottom of the syringe with a peristaltic pump, inject the bacterial solution at a rate of 6ml / min, let it stand for 3 hours after filling, and inject the mixed solution at a rate of 20ml / min after the ba...

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Abstract

The invention discloses a microbial gelled material and a method for forming magnesite by using the microbial gelled material to glue sand grains. The microbial gelled material consists of basophilic microbial broth and mixed solution, wherein the volume ratio of the basophilic microbial broth and the mixed solution is 1:1, the basophilic microbial broth is Sporosarcina pasteurii broth, and the mixed solution is obtained by mixing equal volumes of urea and aqueous solution of MgCl2.6H2O. The method for forming the magnesite comprises formulating 70-90g sand in two grading of lower than 0.15mm and 0.15-0.30mm according to a Fuhlen densest packing method, placing the sand into a test mold, and alternately injecting the basophilic microbial broth in the microbial gelled material into the test mold from top to bottom by using a peristaltic pump. After continuous injection for 10-15 days, the sand grains in the test mold are glued to form the magnesite. The compressive strength of the magnesite is up to 4.0 MPa.

Description

technical field [0001] The invention relates to a new type of cementitious material, in particular to a method for cementing sand particles using magnesite induced by microorganisms. Background technique [0002] At present, the materials used to cement loose particles are artificial chemical materials such as Portland cement, lime, gypsum, water glass and epoxy resin. These materials, except epoxy resin, all have the impact of polluting the environment and destroying the ecology. Among them, Portland cement is the most commonly used cementing material. In 2010, my country's cement production reached 1.868 billion tons, accounting for more than half of the world's cement production. The high energy consumption, high pollution and limitation of limestone resources in the cement production process have become major issues affecting sustainable development. Therefore, there is an urgent need to find new cementing materials that can partially replace traditional cement, reliev...

Claims

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Application Information

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IPC IPC(8): C04B9/00C04B28/30
Inventor 钱春香荣辉李龙治
Owner SOUTHEAST UNIV
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