Efficient phosphorus-dissolution promotion bacteria, microbial agent prepared from same and application of microbial agent

A technology of microbial inoculants and composite microbial inoculants, which are applied in the directions of microorganisms, biocides, and microorganism-based methods, can solve the problems such as no reports of phosphorus-dissolving microbial inoculants and scarcity of phosphorus-dissolving microbial strain resources, etc. Huge agricultural application value, efficient dissolution of insoluble phosphorus, and the effect of improving the utilization rate of phosphorus fertilizer

Inactive Publication Date: 2012-07-04
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Widely adaptable (soil, crop) phosphorus-dissolving microbial strain resources are scarc

Method used

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  • Efficient phosphorus-dissolution promotion bacteria, microbial agent prepared from same and application of microbial agent
  • Efficient phosphorus-dissolution promotion bacteria, microbial agent prepared from same and application of microbial agent
  • Efficient phosphorus-dissolution promotion bacteria, microbial agent prepared from same and application of microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 Isolation and cultivation of Pantoea agglomerans P36

[0055] 1. Cultivation of Phosphorus-Solubilizing Promoting Pantoea agglomerans P36 and Preparation of Phosphorus-Solubilizing Bacteria P36 Inoculant

[0056] (1) Pantoea agglomerans P36 medium

[0057] Fermentation broth formula (kg / t fermentation broth): dipotassium hydrogen phosphate 0.1, potassium dihydrogen phosphate 0.1, sodium chloride 4.3, magnesium sulfate 0.1, starch 8.6, beef extract 4.3, peptone 4.3, yeast powder 4.3, sucrose 8.6, sulfuric acid Ammonium 2.6, calcium carbonate 10, ferrous sulfate 0.001, soybean oil 6.8, prepared with water.

[0058] (2) Cultivation process of Pantoea agglomerans P36

[0059] The P36 strain of Pantoea agglomerans was inoculated on beef juice peptone medium, cultured at 28-30°C for 48h, then inserted into a 1000mL Erlenmeyer flask, and cultured at 30°C and 220r / min for 36h. Insert it into a 50L seed tank according to 5% inoculum amount, and at 220r / min, pH7.5, v...

Embodiment 2

[0062] Embodiment 2 Preparation of efficient phosphorus-dissolving, disease-resistant, and production-increasing compound microbial bacterial agents

[0063] 1. Cultivation of Trichoderma harzianum

[0064] Trichoderma harzianum T-315, deposited in the American Collection of Type Microorganisms (ATCC), with accession number ATCC 20671.

[0065] (1) Composition of medium (kg / t fermentation liquid)

[0066] Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH 4 ) 2 SO 4 3kg, K 2 HPO 4 0.2kg, NaCl 2.5kg, MgSO 4 ·7H 2 O 0.1kg, CaCO 30.5kg, FeSO 4 0.001kg, boric acid (1%) 1L, pH 7.0, prepared with water.

[0067] (2) Fermentation and cultivation process

[0068] Trichoderma harzianum T-315 was inoculated on potato sucrose (PDA) medium and cultured at 25-28°C for 3 days. The spores of Trichoderma harziani were transferred into a 1000mL Erlenmeyer flask, and cultured for liquid fermentation at 26-28°C and 220r / ...

experiment example 1

[0073] Experimental example 1 Phosphorus dissolving effect under the condition of Pantoea agglomerans P36 culture dish

[0074] 1. Pantoea agglomerans P36 was isolated and screened from rhizosphere soil samples collected from the Jinping Phosphate Mine Area in Jiangsu, my country. At the same time, phosphorus-soluble strains P36, P31, P29-5, P40 and P40-2. Under the conditions of indoor solid culture dish and liquid culture medium, their phosphorus-dissolving ability was determined.

[0075] The ratio of the phosphorus-dissolving circle to the colony diameter of the phosphorus-dissolving strain P36 reached 2.83, and the water-soluble soluble (P 2 o 5 ) was 2.21mg; the ratio of the P31 phosphorus-dissolving circle to the colony diameter reached 2.50, and the water-soluble soluble (P 2 o 5 ) was 1.96mg; the ratio of P29-5 phosphorus-dissolving circle to colony diameter reached 2.81, and the water-soluble soluble (P 2 o 5 ) is 2.20 mg; the phosphorus-dissolving circle of bac...

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Abstract

The invention provides efficient phosphorus-dissolution promotion bacteria and a microbial agent prepared from the same. The effective components of the microbial agent include Pantoea agglomerans P36 and Trichoderma harzianum. In the invention, the widely-adaptable efficient phosphorus-dissolution yield-increasing bacteria Pantoea agglomerans P36 and the efficient anti-crop disease Trichoderma harzianum are organically combined; by applying the composite bacterial agent provided by the invention, the effect of the microorganism in preventing soil-borne diseases is sufficiently exerted while efficiently utilizing the soil phosphorus and improving the utilization rate of phosphate fertilizer; an effect of increasing yield by dissolving phosphorus and resisting diseases is realized; and theinvention has tremendous application values in agriculture.

Description

technical field [0001] The invention belongs to the field of microbial inoculum, in particular, relates to a high-efficiency phosphorus-dissolving and growth-promoting bacterium, a microbial inoculum prepared therefrom and its application. Background technique [0002] (1) Development Status of Phosphorus-Solubilizing Microbial Agents [0003] More than 90% of the cultivated land in my country is deficient in phosphorus, and the use of phosphorus fertilizer is the main measure to solve the problem of crop yield increase. Phosphorus fertilizer has increasingly become a bottleneck restricting the sustainable development of agriculture in our country. my country is the world's largest consumer of chemical fertilizers and the largest consumer of phosphate fertilizers, with an annual consumption of about 11 million tons of phosphate fertilizers. As we all know, my country is short of phosphate rock resources, with reserves of 15.198 billion tons. In another 20 years, the medium ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/04A01P3/00C05G3/00C12R1/01
Inventor 范丙全
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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