Method for extracting cottonseed protein from cottonseed meal

Abstract

The invention relates to a method for extracting cottonseed protein. The method comprises the following steps: (1), smashing cottonseeds; (2), treating the cottonseeds with ultrasonic waves; (3), extracting active enzyme in cotton bollworms; (4), performing enzymolysis to cottonseed meal; (5), increasing the temperature, inactivating the enzyme, and centrifugating to obtain supernatant liquid andfilter residue; (6), adding water to flush the filter residue, and mixing the liquid after the filter residue is flushed with the supernatant liquid obtained in the step (3); and (7), removing impurities from the mixed supernatant liquid through a micro filter membrane, condensing through a nanofiltration membrane, and drying to obtain the powdery cottonseed protein. The method provided by the invention can ensure a higher extraction rate in the production of cottonseed protein, can enable the product to have a stronger functional activity, and meanwhile solves the problem that the conventional biological enzymolysis process scheme requires to purchase costly protease, resulting in a high production cost.

Description

[0001] technical field [0002] The invention relates to a method for extracting cottonseed protein, and mainly relates to a method for extracting cottonseed protein by using cottonseed meal as raw material, using intestinal enzyme of cotton bollworm as enzyme preparation and combining ultrasonic technology and membrane separation technology. [0003] Background technique [0004] Cottonseed meal after extracting oil from cottonseed contains a large amount of protein, its content is about 50%, and it is an extremely rich vegetable protein resource. The properties of cottonseed protein are similar to those of soybean protein, and it is also a protein food or feed protein source with high nutritional value and high quality. [0005] The proteins in cottonseed meal are mainly divided into three types. The 2S protein is free outside the cell wall, accounting for about 15%, and the remaining 7S and 12S proteins exist in the cell wall about 85%. Since the cell wall of cottonseed...

AI Technical Summary

Problems solved by technology

[0009] In order to make the production of cottonseed protein have a higher extraction rate, and the product has strong functional activity, and at the same time solve the problem that the existing biological enzymatic hydrolysis process needs to purchase protease at a high price, resulting in high production costs, the present invention provides a method using The active enzyme secreted by the intestinal tract of cotton bollworm is used as an enzyme preparation, and the method of extracting cottonseed protein is combined with ultrasonic technology and membrane separation technology