Method for extracting cottonseed protein from cottonseed meal

A technology for cottonseed protein and cottonseed meal, which is applied in the field of cottonseed protein extraction, can solve the problem of high production cost, and achieve the effects of reducing production cost, increasing solubility and shortening extraction time.

Inactive Publication Date: 2012-07-11
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In order to make the production of cottonseed protein have a higher extraction rate, and the product has strong functional activity, and at the same time solve the problem that the existing biological enzymatic hydrolysis process needs to purchase protease at a high price, resulting ...

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0029] Step (1), take 10kg cottonseed meal, pulverize, particle size is 60 mesh;

[0030] Step (2), add 100kg of water, heat up to 40°C, and ultrasonically treat at 25kHZ frequency for 40min;

[0031] Step (3), take 150g of cotton bollworm, wash, mash, homogenate, dilute with water 5 times the weight of the insect pulp, adjust the pH value to 5.0, extract with 30kHZ frequency ultrasonic wave for 100s, and the supernatant after centrifugation is the active enzyme liquid;

[0032] Step (4), heat up the system after ultrasonic treatment in step (2) to 55°C, adjust the pH value to 7.5, add 500 g of active enzyme extract of cotton bollworm obtained in step (3), and perform enzymatic hydrolysis for 1 hour;

[0033] Step (5), heat up the system obtained after enzymolysis in step (4) to 85°C to inactivate the enzyme, and then centrifuge to obtain supernatant and filter residue respectively;

[0034] Step (6), adding the filter residue obtained in step (5) to 5 times the weight of co...

Embodiment 2

[0040] Step (1): Take 10kg of cottonseed meal and grind it to a particle size of 100 mesh;

[0041] Step (2): Add 100kg of water, heat up to 50°C, and ultrasonically treat at 35kHZ frequency for 60min;

[0042] Step (3): Take 200g of cotton bollworm, wash, mash, homogenate, dilute with water 5 times the weight of the pulp, adjust the pH to 6.0, extract with 40kHZ frequency ultrasonic wave for 100s, and the supernatant after centrifugation is the active enzyme liquid;

[0043] Step (4): Heat up the system after the ultrasonic treatment in step (2) to 60°C, adjust the pH value to 8.0, add 800 g of active enzyme extract of cotton bollworm obtained in step (3), and perform enzymatic hydrolysis for 2 hours;

[0044] Step (5): Heat the system obtained after enzymatic hydrolysis in step (4) to 85°C to inactivate the enzyme, and then centrifuge to obtain supernatant and filter residue respectively;

[0045]Step (6): add the filter residue obtained in step (5) to water 5 times the we...

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Abstract

The invention relates to a method for extracting cottonseed protein. The method comprises the following steps: (1), smashing cottonseeds; (2), treating the cottonseeds with ultrasonic waves; (3), extracting active enzyme in cotton bollworms; (4), performing enzymolysis to cottonseed meal; (5), increasing the temperature, inactivating the enzyme, and centrifugating to obtain supernatant liquid andfilter residue; (6), adding water to flush the filter residue, and mixing the liquid after the filter residue is flushed with the supernatant liquid obtained in the step (3); and (7), removing impurities from the mixed supernatant liquid through a micro filter membrane, condensing through a nanofiltration membrane, and drying to obtain the powdery cottonseed protein. The method provided by the invention can ensure a higher extraction rate in the production of cottonseed protein, can enable the product to have a stronger functional activity, and meanwhile solves the problem that the conventional biological enzymolysis process scheme requires to purchase costly protease, resulting in a high production cost.

Description

[0001] technical field [0002] The invention relates to a method for extracting cottonseed protein, and mainly relates to a method for extracting cottonseed protein by using cottonseed meal as raw material, using intestinal enzyme of cotton bollworm as enzyme preparation and combining ultrasonic technology and membrane separation technology. [0003] Background technique [0004] Cottonseed meal after extracting oil from cottonseed contains a large amount of protein, its content is about 50%, and it is an extremely rich vegetable protein resource. The properties of cottonseed protein are similar to those of soybean protein, and it is also a protein food or feed protein source with high nutritional value and high quality. [0005] The proteins in cottonseed meal are mainly divided into three types. The 2S protein is free outside the cell wall, accounting for about 15%, and the remaining 7S and 12S proteins exist in the cell wall about 85%. Since the cell wall of cottonseed...

Claims

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Application Information

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IPC IPC(8): A23J1/14A23K1/14A23K1/16
Inventor 万端极李冬生汤亚杰吴正奇刘玲海文英肖诗英司佳王雄
Owner HUBEI UNIV OF TECH
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