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Process for extracting and separating flavonoid glycoside from meehania urticaefolia

A technology of Chinese herb and flavonoid glycosides, applied in the field of extraction and separation technology of flavonoid glycosides, can solve the problems of lack of research data on chemical components of Chinese herbal medicine and the like

Inactive Publication Date: 2014-03-26
SHANDONG UNIV AT WEIHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are few research data about the chemical constituents of Chinese hancao

Method used

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  • Process for extracting and separating flavonoid glycoside from meehania urticaefolia
  • Process for extracting and separating flavonoid glycoside from meehania urticaefolia

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. The extraction and separation process of a kind of flavonoid glycoside in American Hancao is characterized in that the process includes the following processes:

[0015] Step (1): Weigh 2.25 kg of dry coarse powder of S. chinensis, add 22.5 kg of 50% ethanol to soak overnight, then ultrasonically extract for 30 minutes, ultrasonic temperature is 30°C, ultrasonic power is 80W, ultrasonic extraction is performed three times, the extracts are combined and Concentrate under reduced pressure, place the concentrated solution in a refrigerator at 4°C for 48 hours, and filter with suction to obtain a concentrated solution from which part of the pigmentation has been removed;

[0016] Step (2): The concentrated solution in step (1) is extracted three times with petroleum ether, ethyl acetate, water-saturated n-butanol and other solvents respectively, and each extraction part is concentrated to a thick paste-like extract, and the solvent is recovered. This obtains n-butanol ex...

Embodiment 2

[0022] 1. The extraction and separation process of a kind of flavonoid glycoside in American Hancao is characterized in that the process includes the following processes:

[0023] Step (1): Weigh 2.25 kg of dry coarse powder of Chinese herba chinensis, add 23.0 kg of 50% ethanol to soak overnight, then ultrasonically extract for 35 minutes, ultrasonic temperature is 32°C, ultrasonic power is 80W, ultrasonic extraction is performed three times, the extracts are combined and Concentrate under reduced pressure, place the concentrated solution in a refrigerator at 4°C for 60 hours, and filter with suction to obtain a concentrated solution from which part of the pigmentation has been removed;

[0024] Step (2): The concentrated solution in step (1) is extracted three times with petroleum ether, ethyl acetate, water-saturated n-butanol and other solvents respectively, and each extraction part is concentrated to a thick paste-like extract, and the solvent is recovered. This obtains n...

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Abstract

The invention belongs to the technical field of medical product research and development, and relates to a process for extracting and separating flavonoid glycoside from meehania urticaefolia. The process comprises the following steps of: weighing dried coarse powder of the meehania urticaefolia, soaking in 50 percent ethanol overnight, extracting by using ultrasonic waves, mixing extracting solutions, concentrating under reduced pressure, extracting the concentrated solution by using solvents such as petroleum ether, ethyl acetate, water-saturated butanol and the like respectively, thus obtaining a butanol extract; performing silica column chromatography, eluting the butanol extract by using different gradient chloroform-methanol, separating to obtain a white precipitate, and repeatedly crystallizing by using methanol to obtain a mono compound; and combining spectral analysis to identify that the structure of the mono compound is 5,3'-dihydroxyl-4'-methoxyl-flavone-7-O-alpha-L-pyran rhamnose-(1->6)-beta-D-glucopyranose. By the process, the demand for the flavonoid glycoside in production and scientific research is met, the yield is improved, and the cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of research and development of pharmaceutical products. The invention relates to an extraction and separation process of a flavonoid glycoside in Chinese hancao. Background technique [0002] Meehania is the dry aerial part of Meehania urticifolia (Miq.) Makino, a plant of the genus Meehania in the Labiatae family. Cimicifuga (Sichuan), a perennial herb, distributed in Tonghua, Hunjiang, Liuhe, Ji'an, Fusong, Jingyu, Jiaohe, Shulan, Antu, Dunhua, Changbai Korean Autonomous Region, Tonghua County and other places, North Korea , Japan is also distributed. American Hancao is pungent, bitter, and slightly cold in nature. The whole herb is used as medicine and can be used as folk medicine. For cold and fever, diarrhea and abdominal pain, hepatitis, cholecystitis, dysuria, snake bites and other diseases. It is used as Chinese herbal medicine in the folk, and the whole herb can be used as medicine. As a cold me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 张崇禧张成城李小叶侯欣池郑友兰
Owner SHANDONG UNIV AT WEIHAI