LAMP (Loop-Mediated Isothermal Amplification) detection kit for marteilia refringens of oyster

Abstract

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) detection kit for marteilia refringens of oyster. Two specific internal primers, two specific external primers and two specific annular primers are designed by using the loop-mediated isothermal amplification (LAMP) technology and according to a common gene conservative region of the marteilia refringens. By applying the LAMP detection kit, the marteilia refringens of the oyster can be detected by only performing DNA (Deoxyribonucleic Acid) extraction and LAMP on a tissue sample, so that the defects of long detection time, high workload, cross contamination, complex operation and the like existing in the prior art are overcome; and the LAMP detection kit has the advantages of high specificity, high sensitivity, quickness, low cost, simpler operation method and is particularly suitable for the requirement of basic level field rapid detection of the marteilia refringens of the oyster.

Description

technical field [0001] The invention relates to the field of oyster refraction Malteia detection, in particular to an oyster refraction Malteia LAMP detection kit. Background technique [0002] Marteilia refringens is very harmful to oyster culture. It can cause shellfish to lose weight, change color of digestive tract, stop growing and die. Its infection phenomenon is common in countries such as Oceania and Europe. [0003] Traditional detection methods for refraction Malteia include electron microscope observation, histocytological examination, in situ hybridization, etc. These methods are time-consuming, laborious, lack of specificity, and have certain limitations in practical work. At present, although the detection methods of refraction Malteia PCR and fluorescent quantitative PCR have been established, their detection sensitivity is relatively high, but conventional PCR requires the use of a PCR machine and gel electrophoresis, and fluorescent quantitative PCR requires...

AI Technical Summary

Problems solved by technology

[0003] The traditional detection methods of refraction Malteia include electron microscope observation, histocytological examination, in situ hybridization, etc. These methods are time-consuming, labor-intensive, lack of specificity, and have certain limitations in practical work

At present, although the detection methods of refraction Malteia PCR and fluorescent quantitative PCR have been established, their detection sensitivity is relatively high, but conventional PCR requires the use of a PCR machine and gel electrophoresis, and fluorescent quantitative PCR requires the use of more expensive Fluorescence quantitative PCR instrument and reagents, etc., so it is difficult to meet the needs of grassroots on-site detection

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