Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof

A technology of nanofiber membrane and silk fibroin, which is applied in the field of silk fibroin nanofiber membrane and its preparation, which can solve the problems of slow oxidation and achieve the effects of preventing damage, improving utilization rate and reducing burden

Inactive Publication Date: 2012-07-11
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But it slowly oxidizes in the...

Method used

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  • Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof
  • Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof
  • Silk fibroin nano-fiber film loaded with vitamins A and E and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) De-chrysalis cocoons were placed in 0.5wt% Na 2 CO 3 Boil in aqueous solution for 2 times, degumming for 30 minutes each time, and obtain silk fibroin by degumming, and use CaCl with a molar ratio of 1:2:8 2 、C 2 h 5 OH and H 2 O was dissolved at 60°C for 1.5 h, and then freeze-dried to obtain loose porous solid silk fibroin.

[0030] (2) Accurately weigh 0.16 grams of TPGS and 0.02 grams of vitamin A palmitate, heat until melted, stir evenly with a magnetic force, then heat 1.44ml of deionized water at 90°C, stir for 15 minutes, remove the heat source, and stir until it becomes transparent , light yellow liquid, add 1.0g silk fibroin and deionized water to the vitamin A-vitamin E emulsion, the final mass is 4.0g, seal and continue to stir until the silk fibroin is completely dissolved, then let it stand for a few minutes before spinning The liquid is transferred to the syringe, the voltage is 20kV, the injection pump advance speed is 0.3ml / h, aluminum foil is ...

Embodiment 2

[0032] (1) De-chrysalis cocoons were placed in 0.5wt% Na 2 CO 3 Boil in the aqueous solution for 4 times, each time for 30 minutes for degumming to obtain silk fibroin, dissolve in 0.8g / ml LiBr aqueous solution at 45°C for 2h, and freeze-dry to obtain loose porous solid silk fibroin.

[0033](2) Accurately weigh 0.32 grams of TPGS and 0.08 grams of vitamin A palmitate, heat until melted, stir evenly with a magnetic force, then heat 1.2ml of deionized water at 90°C, stir for 15 minutes, remove the heat source, and stir until it becomes transparent , light yellow liquid, add 1.0g silk fibroin and deionized water to the vitamin A-vitamin E emulsion, the final mass is 4.0g, seal and continue to stir until the silk fibroin is completely dissolved, then let it stand for a few minutes before spinning The liquid is transferred to the syringe, the voltage is 18kV, the advance speed of the syringe pump is 0.4ml / h, aluminum foil is used to receive, and the receiving distance is 18cm. Th...

Embodiment 3

[0035] (1) De-chrysalis cocoons were placed in 0.5wt% Na 2 CO 3 Boil in the aqueous solution for 5 times, each time for 30 minutes for degumming to obtain silk fibroin, dissolve in 0.8g / ml LiBr aqueous solution at 45°C for 2h, and freeze-dry to obtain loose porous solid silk fibroin.

[0036] (2) Accurately weigh 0.016 grams of TPGS and 0.04 grams of vitamin A palmitate, heat until melted, and stir evenly with a magnetic force, then heat 1.2ml of deionized water at 85°C, stir for 15 minutes, remove the heat source, and stir until it becomes transparent , yellowish liquid, add 1.5g silk fibroin and deionized water to the vitamin A-vitamin E emulsion, the final mass is 4.0g, seal and continue to stir until the silk fibroin is completely dissolved, then let it stand for a few minutes before spinning The liquid is transferred to the syringe, the voltage is 18kV, the advancing speed of the syringe pump is 0.4ml / h, aluminum foil is used to receive, and the receiving distance is 18c...

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Abstract

The invention relates to a silk fibroin nano-fiber film loaded with vitamins A and E and a preparation method thereof. The nano-fiber film comprises silk fibroin nano-fiber, as well as the fat-soluble vitamin A and the water-soluble vitamin E, which are loaded in the silk fibroin nano-fiber. The preparation method comprises the following steps of: (1) heating the vitamins A and E till melting, uniformly stirring, then adding deionized water at the temperature of 80-100 DEG C, and stirring for 10-20min till transparent liquid, namely an emulsion of the vitamins A and E, is formed; (2) adding silk fibroin and the deionized water into the emulsion of the vitamins A and E till the final concentration, and stirring till the complete solution of the silk fibroin so as to get a mixed solution of the three; and (3) transferring the solution into a syringe for performing electrostatic spinning to get the silk fibroin nano-fiber film. The silk fibroin nano-fiber film disclosed by the invention has the advantages of no pollution, low cost and simple operation, and the nano-fiber disclosed by the invention can be applied to artificial skin, medical dressings, hemostatic materials, skin care products and other various biomedical fields.

Description

technical field [0001] The invention belongs to the field of silk fibroin nanofiber membrane and its preparation, in particular to a silk fibroin nanofiber membrane loaded with vitamins A and E and a preparation method thereof. Background technique [0002] Due to the characteristics of small size, large specific surface area and excellent physical properties, nanofibers have broad application prospects in the field of biomedicine. Nanofibrous membranes with small pore size and high specific surface area can promote the hemostatic response without the use of hemostatic agents. The porous structure of the nanofiber membrane is conducive to the respiration of cells without causing dryness of the wound. At the same time, the small pore size effectively prevents the invasion of bacteria, and has good air permeability and oxygen permeability. Changing the dressing brings the chance of interfering with nerve tissue regeneration. Electrospun nanofibers can give skin cells a bette...

Claims

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Application Information

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IPC IPC(8): D04H1/42D04H1/728D01D1/02D01D5/00A61L15/32A61L15/44A61L27/60A61K8/02A61K8/67A61K8/64A61Q19/00C07K1/14
Inventor 王红声盛晓悦范林鹏何创龙莫秀梅崔呈俊韩峰
Owner DONGHUA UNIV
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