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Quality standard of standardized bupleurum extract

A technology of extract and bupleurum, which is applied in the field of standardization and preparation of Chinese herbal medicine extracts, can solve the problems of low extraction rate, poor therapeutic effect, and loss of medicinal components, and achieve good reproducibility, strong specificity, The effect of reliable product quality

Inactive Publication Date: 2012-07-11
吉林人参研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During conventional extraction, especially water extraction, extraction under such environmental conditions can break the epoxy ether bond of protoglycoside, and the extraction rate of the main medicinal ingredients saikosaponin (a, b, c, d) is extremely low , TLC inspection and HPLC detection, there are almost no spots and characteristic absorption peaks of saikosaponin (a, b, c, d), the main medicinal ingredients are lost, and the curative effect is poor

Method used

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  • Quality standard of standardized bupleurum extract
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  • Quality standard of standardized bupleurum extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Three batches of stability scale-up tests

[0033] Standardized extract of Bupleurum radix semi-bionic combined ultrasonic preparation method: Take 250g of Bupleurum chinensis and crush it into a coarse powder, add appropriate amount of 60% ethanol, adjust the pH to 8 with a stabilizer (1.5% KOH), infiltrate for 8 hours, and store at a temperature of 40°C , ultrasonic (power 250W, 50kHz) extraction 3 times, 40 minutes each time, add 8 times the amount of ethanol each time, adjust PH=8 with a stabilizer (1.5% KOH), filter, combine the filtrates, reclaim the ethanol until there is no alcohol smell , concentrated to a relative density of 1.10 (60 ° C) spray drying or microwave vacuum drying, that is (see Table 2).

[0034] The average powder extraction rate of three batches of scale-up experiments was 15.35%, the average extract content was 1.874%, the average yield was 0.288%, and the extraction rate was 84.706% based on the total content of saikosaponin abc...

Embodiment 2

[0047] The quality standards of Bupleurum extract include the identification of Bupleurum extract, the determination of saikosaponin content, the characteristic spectrum and the determination of characteristic peak matching spectrum and other items.

[0048] The method for identification and content determination of Bupleurum bupleuri extract, the steps are as follows:

[0049] (1) Preparation of Bupleurum extract: take 250 g of Bupleurum chinensis and crush it into a coarse powder, add an appropriate amount of 60% ethanol, adjust the pH to 8 with a stabilizer (1.5% KOH), infiltrate for 8 hours, and at a temperature of 40 ° C, ultrasonic ( Power 250W, 50kHz) extraction 3 times, 40 minutes each time, add 8 times the amount of ethanol each time, adjust the pH=8 with a stabilizer (1.5% KOH), filter, combine the filtrates, recover the ethanol until it has no alcohol smell, concentrate to Relative density 1.10 (60 ℃) spray drying or microwave vacuum drying, that is (see Table 2). ...

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Abstract

The invention relates to a method for controlling the quality of a standardized extract of a Chinese herbal medicine, i.e., the quality standard of standardized bupleurum extract. The method comprises the following steps: the bupleurum chinense is extracted in the semi-bionic environment, the total content of the main effective components (saikosaponin a, saikosaponin b, saikosaponin c and saikosaponin d) of the extract is equal to or greater than 1.874 percent, and the total content is equal to or greater than 1.3 percent according to the standard content index. The quality standard of the standardized bupleurum extract includes identification of bupleurum extract, content determination of saikosaponins, specific chromatogram determination and other detection items. The main components of the bupleurum extract can be determined qualitatively and quantitatively, the unified and controllable quality and the standardize production of the bupleurum extract can be ensured, the pharmaceutical quality and the therapeutic effect of the bupleurum extract are guaranteed, and a new quality standard is formulated for completely and accurately evaluating the quality of the standardized bupleurum extract on the basis of experimental research. The method has the characteristics of simplicity, convenience, stability and good reproducibility, and is favorable for improving the inspection efficiency.

Description

technical field [0001] The invention relates to a standard and preparation method of extracts of Chinese medicinal materials, namely the quality standard of standardized extracts of Bupleurum bupleuri. Background technique [0002] In the prior art, the dried root of Bupleurum chinense DC. is processed into an extract, which is a yellow-brown powder with slightly fragrant gas and slightly bitter taste. Bupleurum or North Bupleurum, South Bupleurum. The functions and indications of Bupleurum extract are: evacuating and reducing fever, soothing the liver and relieving stagnation, and elevating yang energy. For common cold and fever, alternating cold and heat, distending pain in the sternum and ribs, irregular menstruation, irregular menstruation, uterine prolapse, prolapse of the anus. Bupleurum bupleuri extracts are mostly water extracts in the market, and saikosaponin cannot be detected by HPLC. There is no qualitative and quantitative standard for its active ingredients, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/02
Inventor 宿武林蔡树群杨文志娄子恒杜跃中
Owner 吉林人参研究院
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