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Simulation cultivation soil with target pathogens, and production method and application of simulation cultivation soil with target pathogens

A technology for cultivating soil and bacteria, applied in the field of agricultural microorganism application, can solve the problems of ineffective infection of tobacco plants, reduction in the number of bacteria, labor and time-consuming, etc., and achieve the effects of being conducive to survival, stable number of bacteria, and effective infection.

Active Publication Date: 2014-04-30
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two methods are complicated to operate, labor-intensive and time-consuming, and are generally not suitable for manual inoculation in field
The inoculation of grain cultures is widely used in the field. After inoculation, the bacteria can reproduce and survive for a period of time on the unused grain culture medium, which ensures the full contact between the bacteria and the roots of the tobacco plants, which is conducive to the infection of the bacteria, but also There is a big risk, the bacteria cultivated in grains can easily become the bait of the antagonistic bacteria, leading to the digestion of the bacteria, the reduction of the number of the bacteria, and the inability to effectively infect the tobacco plants and cause disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The simulated cultivation soil was prepared by fermenting the solid culture of black shank pathogen and fermented farmyard manure and humus soil at a ratio of 1:2:4 by volume. First, crush 1 volume of the solid culture of black shank pathogenic bacteria into a 1-3 mm coarse powder, add 2 volumes of farmyard manure fermented with human and livestock manure that has been sterilized and sterilized at 50-55 °C, fully mix and mix well; Then mix 4 volumes of humus soil sterilized at 50-55°C to obtain the simulated cultivation soil with black shank pathogen.

Embodiment 2

[0029] The solid culture of black shank pathogen, peat and rice cultivation soil were prepared in a volume ratio of 1:4:8 to prepare simulated cultivation soil. Firstly, crush 1 volume of the solid culture of black shank pathogen into 1~3mm coarse powder, add 4 volumes of steam-sterilized, sterilized peat, mix thoroughly, and mix well; After the disinfection at ℃ and without insects, the rice cultivation soil was mixed evenly to obtain the simulated cultivation soil with black shank pathogen.

Embodiment 3

[0031] The solid culture of black shank pathogen, decomposed cake fertilizer and bean cultivation soil were prepared in a volume ratio of 1:3.5:7 to prepare simulated cultivation soil. First, crush 1 volume of solid culture of black shank pathogen into 1~3mm coarse powder, add 3.5 volumes of sterilized and decomposed cake fertilizer at 60~65°C, mix thoroughly, and mix well; then mix 7 volumes After being sterilized at 50-55°C and mixed with insect-free bean cultivation soil, the simulated cultivation soil with black shank bacteria can be obtained.

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PUM

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Abstract

The invention discloses simulation cultivation soil with target pathogens, and a production method and application of the simulation cultivation soil with target pathogens. The cultivation soil produced by the method can be used for testing infection resistance of a tobacco variety to Pectobacterium atrosepticum, screening drugs or bio-control agents and natural products for controlling tobacco Pectobacterium atrosepticum. The production method includes: A, breaking pathogen culture solid; B, well mixing the broken pathogen culture solid with culture medium; and C, well mixing the mixture obtained in step B with base soil. Pathogen inoculants in the cultivation soil with target pathogens are stable in quality and controllable in amount, and the cultivation soil can be used for potting and can be subjected to pond application and scattering around tobacco plant roots to serve as artificial pathogenic soil used for field cultivation. By using the pathogen cultivation soil compared with other means, inoculation is simpler, the pathogens live longer, and infection to tobacco plants is more effective.

Description

Technical field [0001] The present invention belongs to the field of agricultural microbial application technology, and specially involves a simulated cultivation soil and their preparation methods and applications with targets. Background technique [0002] Tobacco Black Shank is the main disease in the world's tobacco production.At present, in the laboratory and greenhouses, there are tablets of tablets (Wang Ge, Zheng Xiaobo, Lu Jiayun, etc. in the laboratory and greenhouses.30-35) Methods such as tobacco and black tibial bacteria such as cobrazed solution or swimming spore fluid irrigation roots.The wire block is usually attached to the rhizomes, and the germs invade from the epidermis at the rhizome junction; the mycelium liquid or swimming spore fluid is often artificially artificial to cause some wounds so that the germs can infect from the root.When these two methods are complicated and spent, they are generally not applicable to the artificial vaccination.There are many ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00
Inventor 方敦煌卢秀萍肖炳光殷端
Owner YUNNAN ACAD OF TOBACCO AGRI SCI